A9_VACCW
ID A9_VACCW Reviewed; 108 AA.
AC Q85320;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 23-FEB-2022, entry version 59.
DE RecName: Full=Virion membrane protein A9;
DE Flags: Precursor;
GN OrderedLocusNames=VACWR128; ORFNames=A9L;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3196167; DOI=10.1007/bf01315559;
RA van Meir E., Wittek R.;
RT "Fine structure of the vaccinia virus gene encoding the precursor of the
RT major core protein 4 a.";
RL Arch. Virol. 102:19-27(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION, INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=11000242; DOI=10.1128/jvi.74.20.9701-9711.2000;
RA Yeh W.W., Moss B., Wolffe E.J.;
RT "The vaccinia virus A9L gene encodes a membrane protein required for an
RT early step in virion morphogenesis.";
RL J. Virol. 74:9701-9711(2000).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=17192302; DOI=10.1128/jvi.02631-06;
RA Husain M., Weisberg A.S., Moss B.;
RT "Sequence-independent targeting of transmembrane proteins synthesized
RT within vaccinia virus factories to nascent viral membranes.";
RL J. Virol. 81:2646-2655(2007).
CC -!- FUNCTION: Envelope protein. Required for an early step in virion
CC morphogenesis. {ECO:0000269|PubMed:11000242}.
CC -!- SUBCELLULAR LOCATION: Virion membrane; Single-pass membrane protein.
CC Host cytoplasm. Note=Component of the mature virion (MV) membrane. The
CC mature virion is located in the cytoplasm of infected cells and is
CC probably released by cell lysis. Also found in cytoplasmic virus
CC factories.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:11000242}.
CC -!- SIMILARITY: Belongs to the chordopoxvirinae A9 family. {ECO:0000305}.
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DR EMBL; M27634; AAA69586.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89407.1; -; Genomic_DNA.
DR PIR; B43497; B43497.
DR RefSeq; YP_233010.1; NC_006998.1.
DR SMR; Q85320; -.
DR DNASU; 3707526; -.
DR GeneID; 3707526; -.
DR KEGG; vg:3707526; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR006920; Poxvirus_A9.
DR Pfam; PF04835; Pox_A9; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Host cytoplasm; Late protein; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..108
FT /note="Virion membrane protein A9"
FT /id="PRO_0000099227"
FT TOPO_DOM 23..45
FT /note="Intravirion"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..83
FT /note="Virion surface"
FT /evidence="ECO:0000255"
FT REGION 76..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 84
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine; by host"
FT /evidence="ECO:0000255"
SQ SEQUENCE 108 AA; 12110 MW; 1A09253ECDEA768A CRC64;
MSCYTAILKS VGGLALFQVA NGAIDLCRHF FMYFCEQKLR PNSFWFVVVR AIASMIMYLV
LGIALLYISE QDDKKNTNNA NTNNDSNSNN SNNDKRNESS INSNSSPK