POPA_RALSO
ID POPA_RALSO Reviewed; 344 AA.
AC Q9RBS0; Q52544; Q9RBY0; Q9WXK0;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=Protein PopA1 {ECO:0000303|PubMed:10430949};
DE Contains:
DE RecName: Full=Protein PopA2 {ECO:0000303|PubMed:10430949};
DE Contains:
DE RecName: Full=Protein PopA3 {ECO:0000303|PubMed:10430949};
GN Name=popA; OrderedLocusNames=RSp0877; ORFNames=RS01648;
OS Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum).
OG Plasmid megaplasmid Rsp.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=267608;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-21 AND 96-144,
RP SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND DISRUPTION PHENOTYPE.
RC STRAIN=GMI1000;
RX PubMed=8313899; DOI=10.1002/j.1460-2075.1994.tb06292.x;
RA Arlat M., Van Gijsegem F., Huet J.-C., Pernollet J.-C., Boucher C.A.;
RT "PopA1, a protein which induces a hypersensitivity-like response on
RT specific Petunia genotypes, is secreted via the Hrp pathway of Pseudomonas
RT solanacearum.";
RL EMBO J. 13:543-553(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GMI1000;
RX PubMed=11823852; DOI=10.1038/415497a;
RA Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL Nature 415:497-502(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=8107;
RA Hikichi Y., Ikegami M., Okuno T.;
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=OE1-1;
RA Hikich Y., Kanda A., Hasegawa H., Okuno T.;
RT "popA in Ralstonia solanacearum OE1-1 pathogenic to tobacco.";
RL Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=10430949; DOI=10.1073/pnas.96.16.9368;
RA Rossier O., Wengelnik K., Hahn K., Bonas U.;
RT "The Xanthomonas Hrp type III system secretes proteins from plant and
RT mammalian bacterial pathogens.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:9368-9373(1999).
CC -!- FUNCTION: Acts as a specific hypersensitive response (HR) elicitor. Has
CC activity on tobacco (non-host plant) and petunia but is without
CC activity on tomato (host plant); PopA3 seems to be more active than a
CC PopA1-PopA2 mixture. {ECO:0000269|PubMed:8313899}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10430949,
CC ECO:0000269|PubMed:8313899}. Note=Probably secreted via a type III
CC secretion system (TTSS). {ECO:0000269|PubMed:10430949,
CC ECO:0000269|PubMed:8313899}.
CC -!- PTM: PopA2 and PopA3 are produced from PopA1.
CC {ECO:0000269|PubMed:8313899}.
CC -!- MASS SPECTROMETRY: [Protein PopA1]: Mass=33033.2; Mass_error=5.2;
CC Method=Electrospray; Note=Mass of PopA1.;
CC Evidence={ECO:0000269|PubMed:8313899};
CC -!- MASS SPECTROMETRY: [Protein PopA3]: Mass=23540.2; Mass_error=7.9;
CC Method=Electrospray; Note=Mass of PopA3.;
CC Evidence={ECO:0000269|PubMed:8313899};
CC -!- DISRUPTION PHENOTYPE: Remains fully pathogenic on sensitive plants,
CC suggesting the bacteria produces other pathogenicity factors.
CC {ECO:0000269|PubMed:8313899}.
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DR EMBL; AJ245811; CAB58262.1; -; Genomic_DNA.
DR EMBL; AL646053; CAD18028.1; -; Genomic_DNA.
DR EMBL; AB026629; BAA77270.1; -; Genomic_DNA.
DR EMBL; AB032747; BAA84679.1; -; Genomic_DNA.
DR RefSeq; WP_011004174.1; NC_003296.1.
DR AlphaFoldDB; Q9RBS0; -.
DR STRING; 267608.RSp0877; -.
DR EnsemblBacteria; CAD18028; CAD18028; RSp0877.
DR GeneID; 60503785; -.
DR KEGG; rso:RSp0877; -.
DR PATRIC; fig|267608.8.peg.4347; -.
DR HOGENOM; CLU_806250_0_0_4; -.
DR OMA; QGPQSAN; -.
DR PHI-base; PHI:5145; -.
DR Proteomes; UP000001436; Plasmid megaplasmid Rsp.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IDA:UniProtKB.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Hypersensitive response elicitation; Plasmid;
KW Reference proteome; Secreted.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8313899"
FT CHAIN 2..344
FT /note="Protein PopA1"
FT /evidence="ECO:0000269|PubMed:8313899"
FT /id="PRO_0000022085"
FT CHAIN 10..344
FT /note="Protein PopA2"
FT /evidence="ECO:0000303|PubMed:8313899"
FT /id="PRO_0000022086"
FT CHAIN 96..344
FT /note="Protein PopA3"
FT /evidence="ECO:0000269|PubMed:8313899"
FT /id="PRO_0000022087"
FT REGION 1..28
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 134..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 211..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..72
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 79..108
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 211..227
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 24..25
FT /note="Missing (in strain: OE1-1)"
FT VARIANT 80..85
FT /note="ANDPSK -> GA (in strain: 8107)"
FT VARIANT 173
FT /note="L -> P (in strain: OE1-1)"
FT VARIANT 177
FT /note="G -> A (in strain: 8107 and OE1-1)"
FT VARIANT 187..196
FT /note="Missing (in strain: 8107)"
FT VARIANT 193..195
FT /note="Missing (in strain: OE1-1)"
FT VARIANT 229
FT /note="N -> I (in strain: OE1-1)"
FT VARIANT 269
FT /note="G -> A (in strain: 8107)"
FT VARIANT 305
FT /note="G -> D (in strain: OE1-1)"
FT VARIANT 329
FT /note="A -> E (in strain: OE1-1)"
SQ SEQUENCE 344 AA; 33152 MW; 42A7C5A0C32B0907 CRC64;
MSVGNIQSPS NLPGLQNLNL NTNTNSQQSG QSVQDLIKQV EKDILNIIAA LVQKAAQSAG
GNTGNTGNAP AKDGNANAGA NDPSKNDPSK SQGPQSANKT GNVDDANNQD PMQALMQLLE
DLVKLLKAAL HMQQPGGNDK GNGVGGANGA KGAGGQGGLA EALQEIEQIL AQLGGGGAGA
GGAGGGVGGA GGADGGSGAG GAGGANGADG GNGVNGNQAN GPQNAGDVNG ANGADDGSED
QGGLTGVLQK LMKILNALVQ MMQQGGLGGG NQAQGGSKGA GNASPASGAN PGANQPGSAD
DQSSGQNNLQ SQIMDVVKEV VQILQQMLAA QNGGSQQSTS TQPM
