ID   POPD2_DANRE             Reviewed;         370 AA.
AC   Q6JWV8;
DT   06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Popeye domain-containing 2 {ECO:0000312|ZFIN:ZDB-GENE-060518-2};
GN   Name=popdc2 {ECO:0000312|ZFIN:ZDB-GENE-060518-2};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1] {ECO:0000312|EMBL:AAQ57588.1}
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Brand T., Meyer D., Yelon D.;
RT   "Characterization of the popeye gene family in zebrafish.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3] {ECO:0000312|EMBL:AAI29265.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB {ECO:0000312|EMBL:AAI29265.1};
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=22290329; DOI=10.1016/j.ydbio.2012.01.015;
RA   Kirchmaier B.C., Poon K.L., Schwerte T., Huisken J., Winkler C.,
RA   Jungblut B., Stainier D.Y., Brand T.;
RT   "The Popeye domain containing 2 (popdc2) gene in zebrafish is required for
RT   heart and skeletal muscle development.";
RL   Dev. Biol. 363:438-450(2012).
CC   -!- FUNCTION: Important for striated muscle differentiation and cardiac
CC       morphogenesis. Is also required for cardiac conduction system
CC       development, plays a regulatory function in heart rate dynamics
CC       mediated, at least in part, through cAMP-binding.
CC       {ECO:0000269|PubMed:22290329}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}. Cell membrane, sarcolemma
CC       {ECO:0000250|UniProtKB:Q9HBU9}.
CC   -!- TISSUE SPECIFICITY: Expressed in the heart and, slightly, in skeletal
CC       muscle. {ECO:0000269|PubMed:22290329}.
CC   -!- DEVELOPMENTAL STAGE: Detectable at 4 hours post-fertilization (hpf),
CC       expression levels peaked at 24 hpf and gradually decreased afterwards.
CC       Expression become detectable when skeletal muscle differentiation
CC       commences at 14-16 somites with an anteropsterior gradient. Myocardial
CC       expression starts at 22 hpf in the forming heart tube and, at 24 hpf,
CC       is confined to differentiated cardiac muscles cells. At 24 hpf, is also
CC       expressed in the entire myotome. At 48 and 72 hpf, expression in the
CC       trunk muscle cells disappeares, but is present in muscles cells of the
CC       pectoral fin bud and facial muscles. {ECO:0000269|PubMed:22290329}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein affects
CC       skeletal muscle development, resulting in abnormal facial and trunk
CC       muscle formation with defective aligment of muscle fibers and
CC       establishment of myotendinous junctions. A severe defect is observed in
CC       craniofacial muscle development, where many muscles are malformed or
CC       reduced in size. In the heart, pericardial edema is prevalent in the
CC       morphants with defective looping and misshaped chambers.
CC       {ECO:0000269|PubMed:22290329}.
CC   -!- SIMILARITY: Belongs to the popeye family. {ECO:0000305}.
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DR   EMBL; BX511129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AY293119; AAQ57588.1; -; mRNA.
DR   EMBL; BC129264; AAI29265.1; -; mRNA.
DR   EMBL; BC164200; AAI64200.1; -; mRNA.
DR   RefSeq; NP_001018636.1; NM_001020800.1.
DR   AlphaFoldDB; Q6JWV8; -.
DR   SMR; Q6JWV8; -.
DR   STRING; 7955.ENSDARP00000092923; -.
DR   PaxDb; Q6JWV8; -.
DR   Ensembl; ENSDART00000102147; ENSDARP00000092923; ENSDARG00000069922.
DR   Ensembl; ENSDART00000162578; ENSDARP00000136769; ENSDARG00000069922.
DR   GeneID; 793666; -.
DR   KEGG; dre:793666; -.
DR   CTD; 64091; -.
DR   ZFIN; ZDB-GENE-060518-2; popdc2.
DR   eggNOG; ENOG502R0XG; Eukaryota.
DR   GeneTree; ENSGT00390000002563; -.
DR   HOGENOM; CLU_048494_2_0_1; -.
DR   InParanoid; Q6JWV8; -.
DR   OMA; FLCLTLW; -.
DR   OrthoDB; 1369469at2759; -.
DR   PhylomeDB; Q6JWV8; -.
DR   TreeFam; TF326644; -.
DR   PRO; PR:Q6JWV8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 9.
DR   Bgee; ENSDARG00000069922; Expressed in heart and 10 other tissues.
DR   ExpressionAtlas; Q6JWV8; baseline.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042383; C:sarcolemma; IBA:GO_Central.
DR   GO; GO:0030552; F:cAMP binding; IBA:GO_Central.
DR   GO; GO:0061337; P:cardiac conduction; IMP:ZFIN.
DR   GO; GO:0007507; P:heart development; IBA:GO_Central.
DR   GO; GO:0001947; P:heart looping; IMP:ZFIN.
DR   GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR   GO; GO:0007519; P:skeletal muscle tissue development; IBA:GO_Central.
DR   GO; GO:0051146; P:striated muscle cell differentiation; IMP:ZFIN.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR006916; Popeye_prot.
DR   PANTHER; PTHR12101; PTHR12101; 1.
DR   Pfam; PF04831; Popeye; 1.
DR   SUPFAM; SSF51206; SSF51206; 1.
PE   2: Evidence at transcript level;
KW   cAMP; cAMP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..370
FT                   /note="Popeye domain-containing 2"
FT                   /id="PRO_0000436602"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          275..349
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        326..343
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   370 AA;  41033 MW;  00EE75432C9C0013 CRC64;
     MNSGDSSLID AVIYNHTLCD GWTNSTEGAI YHLGNTILFL GYMGGSGAYG ALYIFSFLAP
     AFLCLALWGW LTMCGLDVFI WNLLLMLQCL AQVCHLIFRL MRDGLANEEF SALYTAVYLP
     LDVPVQVFKE ITGAGENKVL SLAAEETYAV EGKTPIDQLS FLLSGRIRVS LEGQFLHYIF
     PHQFLDSPEW ESLRPAEEGN FQVTLTAETD CRYISWRRRR LYLLLSKDRY IARLFSVMLG
     SDIADKLYSL NDKLFAKSGV RLDIRLPSLY HVLAPSPPGS EGGSASSPPR GSLGDPTVIK
     VDPAPSNPGS GTRNPQPDQG KNPVPKNPHQ HWSSDTEMPS GEDSTSLILE DFADMTGSLM
     DYGHEREYLK