ID   PORA_HORVU              Reviewed;         388 AA.
AC   P13653;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   25-MAY-2022, entry version 121.
DE   RecName: Full=Protochlorophyllide reductase A, chloroplastic;
DE            Short=PCR A;
DE            EC=1.3.1.33;
DE   AltName: Full=NADPH-protochlorophyllide oxidoreductase A;
DE            Short=POR A;
DE   Flags: Precursor;
GN   Name=PORA;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. Carina; TISSUE=Leaf;
RX   PubMed=2671659; DOI=10.1007/bf02464904;
RA   Schuelz R., Steinmueller K., Klaas M., Forreiter C., Rasmussen S.,
RA   Hiller C., Apel K.;
RT   "Nucleotide sequence of a cDNA coding for the NADPH-protochlorophyllide
RT   oxidoreductase (PCR) of barley (Hordeum vulgare L.) and its expression in
RT   Escherichia coli.";
RL   Mol. Gen. Genet. 217:355-361(1989).
CC   -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC       chlorophyllide (Chlide).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chlorophyllide a + NADP(+) = H(+) + NADPH +
CC         protochlorophyllide a; Xref=Rhea:RHEA:11132, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83348,
CC         ChEBI:CHEBI:83350; EC=1.3.1.33;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- INTERACTION:
CC       P13653; Q42850: PORB; NbExp=2; IntAct=EBI-15724741, EBI-15724755;
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DEVELOPMENTAL STAGE: Active only transiently in etiolated seedlings at
CC       the beginning of illumination.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. POR subfamily. {ECO:0000305}.
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DR   EMBL; X15869; CAA33879.1; -; mRNA.
DR   PIR; S04783; S04783.
DR   AlphaFoldDB; P13653; -.
DR   SMR; P13653; -.
DR   DIP; DIP-46278N; -.
DR   IntAct; P13653; 1.
DR   BRENDA; 1.3.1.33; 2687.
DR   UniPathway; UPA00668; -.
DR   ExpressionAtlas; P13653; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005979; Prochl_reduct.
DR   InterPro; IPR002347; SDR_fam.
DR   PANTHER; PTHR44419; PTHR44419; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   TIGRFAMs; TIGR01289; LPOR; 1.
PE   1: Evidence at protein level;
KW   Chlorophyll biosynthesis; Chloroplast; Direct protein sequencing; NADP;
KW   Oxidoreductase; Photosynthesis; Plastid; Transit peptide.
FT   TRANSIT         1..74
FT                   /note="Chloroplast"
FT   CHAIN           75..388
FT                   /note="Protochlorophyllide reductase A, chloroplastic"
FT                   /id="PRO_0000023293"
FT   REGION          48..69
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   388 AA;  41181 MW;  EBD3EF153D96C129 CRC64;
     MALQLLPSTL SVPKKGSSMG AVAVKDTAAF LGVSSKAKKA SLAVRTQVAT APSPVTTSPG
     STASSPSGKK TLRQGVVVIT GASSGLGLAA AKALAETGKW HVVMACRDFL KASKAAKAAG
     MADGSYTVMH LDLASLDSVR QFVDAFRRAE MPLDVLVCNA AIYRPTARTP TFTADGHEMS
     VGVNHLGHFL LARLLMEDLQ KSDYPSRRMV IVGSITGNSN TLAGNVPPKA SLGDLRGLAG
     GLSGASGSAM IDGDESFDGA KAYKDSKVCN MLTMQEFHRR YHEETGITFS SLYPGCIATT
     GLFREHIPLF RTLFPPFQKF VTKGFVSEAE SGKRLAQVVA EPVLTKSGVY WSWNKDSASF
     ENQLSQEASD PEKARKVWEL SEKLVGLA