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AT8B2_MOUSE
ID   AT8B2_MOUSE             Reviewed;        1209 AA.
AC   P98199;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 156.
DE   RecName: Full=Phospholipid-transporting ATPase ID;
DE            EC=7.6.2.1 {ECO:0000250|UniProtKB:P98198};
DE   AltName: Full=ATPase class I type 8B member 2;
DE   AltName: Full=P4-ATPase flippase complex alpha subunit ATP8B2;
GN   Name=Atp8b2 {ECO:0000303|PubMed:30018401, ECO:0000312|MGI:MGI:1859660};
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 863-1209.
RC   STRAIN=C57BL/6J; TISSUE=Embryo;
RX   PubMed=11015572; DOI=10.1152/physiolgenomics.1999.1.3.139;
RA   Halleck M.S., Lawler J.F. Jr., Blackshaw S., Gao L., Nagarajan P.,
RA   Hacker C., Pyle S., Newman J.T., Nakanishi Y., Ando H., Weinstock D.,
RA   Williamson P.L., Schlegel R.A.;
RT   "Differential expression of putative transbilayer amphipath transporters.";
RL   Physiol. Genomics 1:139-150(1999).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH TMEM30A, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=30018401; DOI=10.1038/s41598-018-29108-z;
RA   Wang J., Molday L.L., Hii T., Coleman J.A., Wen T., Andersen J.P.,
RA   Molday R.S.;
RT   "Proteomic Analysis and Functional Characterization of P4-ATPase
RT   Phospholipid Flippases from Murine Tissues.";
RL   Sci. Rep. 8:10795-10795(2018).
CC   -!- FUNCTION: Catalytic component of P4-ATPase flippase complex, which
CC       catalyzes the hydrolysis of ATP coupled to the transport of
CC       phosphatidylcholine (PC) from the outer to the inner leaflet of the
CC       plasma membrane. May contribute to the maintenance of membrane lipid
CC       asymmetry. {ECO:0000250|UniProtKB:P98198}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + phospholipidSide 1 = ADP + phosphate +
CC         phospholipidSide 2.; EC=7.6.2.1;
CC         Evidence={ECO:0000250|UniProtKB:P98198};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine(out) + ATP + H2O = a
CC         1,2-diacyl-sn-glycero-3-phosphocholine(in) + ADP + H(+) + phosphate;
CC         Xref=Rhea:RHEA:38583, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:57643,
CC         ChEBI:CHEBI:456216; Evidence={ECO:0000250|UniProtKB:P98198};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38584;
CC         Evidence={ECO:0000250|UniProtKB:P98198};
CC   -!- SUBUNIT: Component of a P4-ATPase flippase complex which consists of a
CC       catalytic alpha subunit ATP8B2 and an accessory beta subunit TMEM30A or
CC       TMEM30B. {ECO:0000250|UniProtKB:P98198, ECO:0000269|PubMed:30018401}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:P98198};
CC       Multi-pass membrane protein {ECO:0000255}. Endoplasmic reticulum
CC       membrane {ECO:0000250|UniProtKB:P98198}; Multi-pass membrane protein
CC       {ECO:0000255}. Note=Efficient exit from the endoplasmic reticulum
CC       requires the presence of TMEM30A or TMEM30B.
CC       {ECO:0000250|UniProtKB:P98198}.
CC   -!- TISSUE SPECIFICITY: Expressed in brain and testes (at protein level).
CC       {ECO:0000269|PubMed:30018401}.
CC   -!- SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3)
CC       family. Type IV subfamily. {ECO:0000305}.
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DR   EMBL; AC163616; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AF156546; AAF09444.1; -; mRNA.
DR   CCDS; CCDS89658.1; -.
DR   RefSeq; XP_006501777.1; XM_006501714.3.
DR   AlphaFoldDB; P98199; -.
DR   SMR; P98199; -.
DR   BioGRID; 207713; 1.
DR   STRING; 10090.ENSMUSP00000103019; -.
DR   iPTMnet; P98199; -.
DR   PhosphoSitePlus; P98199; -.
DR   EPD; P98199; -.
DR   jPOST; P98199; -.
DR   MaxQB; P98199; -.
DR   PaxDb; P98199; -.
DR   PRIDE; P98199; -.
DR   ProteomicsDB; 265135; -.
DR   Antibodypedia; 34152; 92 antibodies from 16 providers.
DR   Ensembl; ENSMUST00000069805; ENSMUSP00000063384; ENSMUSG00000060671.
DR   UCSC; uc008qah.1; mouse.
DR   MGI; MGI:1859660; Atp8b2.
DR   VEuPathDB; HostDB:ENSMUSG00000060671; -.
DR   eggNOG; KOG0206; Eukaryota.
DR   GeneTree; ENSGT00940000160804; -.
DR   HOGENOM; CLU_000846_5_2_1; -.
DR   InParanoid; P98199; -.
DR   OrthoDB; 587717at2759; -.
DR   BRENDA; 7.6.2.1; 3474.
DR   Reactome; R-MMU-936837; Ion transport by P-type ATPases.
DR   BioGRID-ORCS; 54667; 2 hits in 71 CRISPR screens.
DR   PRO; PR:P98199; -.
DR   Proteomes; UP000000589; Chromosome 3.
DR   RNAct; P98199; protein.
DR   Bgee; ENSMUSG00000060671; Expressed in humerus cartilage element and 239 other tissues.
DR   ExpressionAtlas; P98199; baseline and differential.
DR   Genevisible; P98199; MM.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR   GO; GO:0005887; C:integral component of plasma membrane; ISO:MGI.
DR   GO; GO:0005730; C:nucleolus; ISO:MGI.
DR   GO; GO:1990531; C:phospholipid-translocating ATPase complex; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR   GO; GO:0005802; C:trans-Golgi network; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0140326; F:ATPase-coupled intramembrane lipid transporter activity; IBA:GO_Central.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0140345; F:phosphatidylcholine flippase activity; ISO:MGI.
DR   GO; GO:0090554; F:phosphatidylcholine floppase activity; IEA:RHEA.
DR   GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR   GO; GO:0045332; P:phospholipid translocation; IBA:GO_Central.
DR   Gene3D; 3.40.1110.10; -; 1.
DR   Gene3D; 3.40.50.1000; -; 1.
DR   InterPro; IPR030347; ATP8B2.
DR   InterPro; IPR023299; ATPase_P-typ_cyto_dom_N.
DR   InterPro; IPR018303; ATPase_P-typ_P_site.
DR   InterPro; IPR023298; ATPase_P-typ_TM_dom_sf.
DR   InterPro; IPR008250; ATPase_P-typ_transduc_dom_A_sf.
DR   InterPro; IPR036412; HAD-like_sf.
DR   InterPro; IPR023214; HAD_sf.
DR   InterPro; IPR006539; P-type_ATPase_IV.
DR   InterPro; IPR032631; P-type_ATPase_N.
DR   InterPro; IPR001757; P_typ_ATPase.
DR   InterPro; IPR032630; P_typ_ATPase_c.
DR   InterPro; IPR044492; P_typ_ATPase_HD_dom.
DR   PANTHER; PTHR24092:SF46; PTHR24092:SF46; 1.
DR   Pfam; PF16212; PhoLip_ATPase_C; 1.
DR   Pfam; PF16209; PhoLip_ATPase_N; 1.
DR   SFLD; SFLDF00027; p-type_atpase; 1.
DR   SUPFAM; SSF56784; SSF56784; 1.
DR   SUPFAM; SSF81653; SSF81653; 1.
DR   SUPFAM; SSF81660; SSF81660; 1.
DR   SUPFAM; SSF81665; SSF81665; 1.
DR   TIGRFAMs; TIGR01652; ATPase-Plipid; 1.
DR   TIGRFAMs; TIGR01494; ATPase_P-type; 1.
DR   PROSITE; PS00154; ATPASE_E1_E2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cell membrane; Endoplasmic reticulum; Lipid transport;
KW   Magnesium; Membrane; Metal-binding; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Translocase; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..1209
FT                   /note="Phospholipid-transporting ATPase ID"
FT                   /id="PRO_0000046366"
FT   TOPO_DOM        1..68
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        90..91
FT                   /note="Exoplasmic loop"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        92..112
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        113..295
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        296..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        317..338
FT                   /note="Exoplasmic loop"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        360..898
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        899..919
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        920..922
FT                   /note="Exoplasmic loop"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        923..943
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        944..972
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        973..993
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        994..1011
FT                   /note="Exoplasmic loop"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1012..1032
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1033..1036
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1037..1057
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1058..1082
FT                   /note="Exoplasmic loop"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1083..1103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1104..1209
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          12..36
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1179..1209
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        411
FT                   /note="4-aspartylphosphate intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9HD20"
FT   BINDING         833
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NB49"
FT   BINDING         837
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250|UniProtKB:Q8NB49"
FT   MOD_RES         1175
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P98198"
FT   CONFLICT        989
FT                   /note="F -> L (in Ref. 2; AAF09444)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1209 AA;  136968 MW;  4722C6D25F280273 CRC64;
     MTVPKEIPEK WARAGAPPSW SQKKPSWGTE EERRARANDR EYNEKFQYAS NCIKTSKYNI
     VTFLPVNLFE QFQEVANTYF LFLLILQLIP QISSLSWFTT IVPLVLVLTI TAVKDATDDY
     FRHKSDNQVN NRHSQVLING VLQQEQWMNV CVGDIIKLEN NQFVAADLLL LSSSEPHGLC
     YIETAELDGE TNMKVRQAIP VTSELGDVSQ LARFDGEVIC EPPNNKLDKF SGTLYWKENK
     FPLSNQNMLL RGCVLRNTEW CFGLVIFAGP DTKLMQNSGR TKFKRTSIDR LMNTLVLWIF
     GFLVCMGVIL AIGNAIWEHE VGTRFQVYLP WDEAVDSAFF SGFLSFWSYI IILNTVVPIS
     LYVSVEVIRL GHSYFINWDK KMFCMKKRTP AEARTTTLNE ELGQVEYIFS DKTGTLTQNI
     MVFNKCSING HSYGDVFDVL GHKAELGERP EPVDFSFNPL ADKKFLFWDS SLLEAVKMGD
     PHTHEFFRLL SLCHTVMSEE KNEGELYYKA QSPDEGALVT AARNFGFVFR SRTPKTITVH
     ELGTAITYQL LAILDFNNIR KRMSVIVRNP EGKIRLYCKG ADTILLDRLH PPTQELLSST
     TDHLNEYAGD GLRTLVLAYK DLDEEYYEEW ARRRLQASLA QDSREDRLAS IYEEVESDMM
     LLGATAIEDK LQQGVPETIA LLTLANIKIW VLTGDKQETA VNIGYSCKML TDDMTEVFVV
     TGHTVLEVRE ELRKARKKMV DSSHAVGNGF TYQGNLSSSK LTSVLEAVAG EYALVINGHS
     LAHALEADME LEFLETACAC KAVICCRVTP LQKAQVVELV KKYKKAVTLA IGDGANDVSM
     IKTAHIGVGI SGQEGIQAVL ASDYSFSQFK FLQRLLLVHG RWSYLRMCKF LCYFFYKNFA
     FTMVHFWFGF FCGFSAQTVY DQYFITLYNI VYTSLPVLAM GVFDQDVPEQ RSMEYPKLYE
     PGQLNLLFNK REFFICIAQG IYTSVLMFFI PYGVFAEATR DDGTQLADYQ SFAVTVATSL
     VIVVSVQIGL DTGYWTAINH FFIWGSLAVY FAILFAMHSN GLFDMFPNQF RFVGNAQNTL
     AQPTVWLTIA LTTAVCIMPV VAFRFLRLSL KPDLSDTVRY TQLVRKKQKA QHRCMRRVGR
     TGSRRSGYAF SHQEGFGELI MSGKNMRLSS LALSSFSTRS SSSWIESLRR KKSDSANSPS
     GGAEKPLKG
 
 
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