PORB_HORVU
ID PORB_HORVU Reviewed; 395 AA.
AC Q42850;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Protochlorophyllide reductase B, chloroplastic;
DE Short=PCR B;
DE EC=1.3.1.33;
DE AltName: Full=NADPH-protochlorophyllide oxidoreductase B;
DE Short=POR B;
DE Flags: Precursor;
GN Name=PORB;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7724548; DOI=10.1073/pnas.92.8.3254;
RA Holtorf H., Reinbothe S., Reinbothe C., Bereza B., Apel K.;
RT "Two routes of chlorophyllide synthesis that are differentially regulated
RT by light in barley (Hordeum vulgare L.).";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3254-3258(1995).
CC -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC chlorophyllide (Chlide).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chlorophyllide a + NADP(+) = H(+) + NADPH +
CC protochlorophyllide a; Xref=Rhea:RHEA:11132, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83348,
CC ChEBI:CHEBI:83350; EC=1.3.1.33;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- INTERACTION:
CC Q42850; P13653: PORA; NbExp=2; IntAct=EBI-15724755, EBI-15724741;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. POR subfamily. {ECO:0000305}.
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DR EMBL; X84738; CAA59228.1; -; mRNA.
DR PIR; S52285; S52285.
DR AlphaFoldDB; Q42850; -.
DR SMR; Q42850; -.
DR DIP; DIP-46279N; -.
DR IntAct; Q42850; 1.
DR PRIDE; Q42850; -.
DR EnsemblPlants; HORVU.MOREX.r2.1HG0036700.1; HORVU.MOREX.r2.1HG0036700.1; HORVU.MOREX.r2.1HG0036700.
DR EnsemblPlants; HORVU.MOREX.r2.1HG0036700.1.mrna1; HORVU.MOREX.r2.1HG0036700.1.mrna1; HORVU.MOREX.r2.1HG0036700.1.
DR Gramene; HORVU.MOREX.r2.1HG0036700.1; HORVU.MOREX.r2.1HG0036700.1; HORVU.MOREX.r2.1HG0036700.
DR Gramene; HORVU.MOREX.r2.1HG0036700.1.mrna1; HORVU.MOREX.r2.1HG0036700.1.mrna1; HORVU.MOREX.r2.1HG0036700.1.
DR BRENDA; 1.3.1.33; 2687.
DR UniPathway; UPA00668; -.
DR ExpressionAtlas; Q42850; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR005979; Prochl_reduct.
DR InterPro; IPR002347; SDR_fam.
DR PANTHER; PTHR44419; PTHR44419; 1.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01289; LPOR; 1.
PE 1: Evidence at protein level;
KW Chlorophyll biosynthesis; Chloroplast; NADP; Oxidoreductase;
KW Photosynthesis; Plastid; Transit peptide.
FT TRANSIT 1..59
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 60..395
FT /note="Protochlorophyllide reductase B, chloroplastic"
FT /id="PRO_0000023294"
SQ SEQUENCE 395 AA; 42148 MW; BFCB90122D6F64B9 CRC64;
MALQAATSFL PSALSARKEG AAKDSAFFGV RLADGLKLDA TSLGLRTKRV NTSSVAIRAQ
AAAVSAPTAT PASPAGKKTV RTGNAIITGA SSGLGLATAK ALAESGKWHV IMACRDYLKT
ARAARAAGMP KGSYTIVHLD LASLDSVRQF VKNVRQLDMP IDVVVCNAAV YQPTAKEPSF
TADGFEMSVG VNHLGHFLLA RELLEDLKAS DYPSKRLIIV GSITGNTNTL AGNVPPKANL
GDLRGLAAGL NGVGSAAMID GAEFDGAKAY KDSKVCNMLT MQEFHRRYHE ETGVTFASLY
PGCIATTGLF REHIPLFRLL FPPFQKYITK GYVSEEEAGK RLAQVVSEPS LTKSGVYWSW
NKNSASFENQ LSEEASDTEK ARKVWELSEK LVGLA
