PORC_PYRFU
ID PORC_PYRFU Reviewed; 185 AA.
AC Q51799;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Pyruvate/ketoisovalerate oxidoreductases common subunit gamma;
DE Includes:
DE RecName: Full=Pyruvate synthase subunit PorC;
DE EC=1.2.7.1;
DE AltName: Full=Pyruvate oxidoreductase gamma chain;
DE Short=POR;
DE AltName: Full=Pyruvic-ferredoxin oxidoreductase subunit gamma;
DE Includes:
DE RecName: Full=Ketoisovalerate oxidoreductase subunit VorC;
DE Short=VOR;
DE EC=1.2.7.7;
DE AltName: Full=2-oxoisovalerate ferredoxin reductase subunit gamma;
DE AltName: Full=2-oxoisovalerate oxidoreductase gamma chain;
GN Name=porG; OrderedLocusNames=PF0971;
OS Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=186497;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-28.
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=8550425; DOI=10.1128/jb.178.1.248-257.1996;
RA Kletzin A., Adams M.W.W.;
RT "Molecular and phylogenetic characterization of pyruvate and 2-
RT ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and
RT pyruvate ferredoxin oxidoreductase from Thermotoga maritima.";
RL J. Bacteriol. 178:248-257(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA DiRuggiero J., Robb F.T.;
RT "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT horikoshii inferred from complete genomic sequences.";
RL Genetics 152:1299-1305(1999).
RN [3]
RP CHARACTERIZATION.
RC STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX PubMed=8380721; DOI=10.1016/0167-4838(93)90190-3;
RA Blamey J.M., Adams M.W.W.;
RT "Purification and characterization of pyruvate ferredoxin oxidoreductase
RT from the hyperthermophilic archaeon Pyrococcus furiosus.";
RL Biochim. Biophys. Acta 1161:19-27(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CoA + 2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate = acetyl-CoA
CC + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:12765,
CC Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33737,
CC ChEBI:CHEBI:33738, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=1.2.7.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-methyl-2-oxobutanoate + CoA + 2 oxidized [2Fe-2S]-
CC [ferredoxin] = 2-methylpropanoyl-CoA + CO2 + H(+) + 2 reduced [2Fe-
CC 2S]-[ferredoxin]; Xref=Rhea:RHEA:11712, Rhea:RHEA-COMP:10000,
CC Rhea:RHEA-COMP:10001, ChEBI:CHEBI:11851, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57338; EC=1.2.7.7;
CC -!- SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma
CC chain.
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DR EMBL; X85250; CAA59500.1; -; Genomic_DNA.
DR EMBL; AE009950; AAL81095.1; -; Genomic_DNA.
DR PIR; T45083; T45083.
DR RefSeq; WP_011012108.1; NC_018092.1.
DR AlphaFoldDB; Q51799; -.
DR SMR; Q51799; -.
DR STRING; 186497.PF0971; -.
DR PRIDE; Q51799; -.
DR EnsemblBacteria; AAL81095; AAL81095; PF0971.
DR GeneID; 41712783; -.
DR KEGG; pfu:PF0971; -.
DR PATRIC; fig|186497.12.peg.1030; -.
DR eggNOG; arCOG01603; Archaea.
DR HOGENOM; CLU_087284_2_0_2; -.
DR OMA; YEIASEW; -.
DR OrthoDB; 95610at2157; -.
DR PhylomeDB; Q51799; -.
DR BRENDA; 1.2.7.1; 5243.
DR Proteomes; UP000001013; Chromosome.
DR GO; GO:0043807; F:3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity; IEA:UniProtKB-EC.
DR GO; GO:0019164; F:pyruvate synthase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.920.10; -; 1.
DR InterPro; IPR011894; PorC_KorC.
DR InterPro; IPR019752; Pyrv/ketoisovalerate_OxRed_cat.
DR InterPro; IPR002869; Pyrv_flavodox_OxRed_cen.
DR Pfam; PF01558; POR; 1.
DR SUPFAM; SSF53323; SSF53323; 1.
DR TIGRFAMs; TIGR02175; PorC_KorC; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Oxidoreductase; Reference proteome.
FT CHAIN 1..185
FT /note="Pyruvate/ketoisovalerate oxidoreductases common
FT subunit gamma"
FT /id="PRO_0000099914"
SQ SEQUENCE 185 AA; 20021 MW; E58743586CE87C13 CRC64;
MIEVRFHGRG GQGAVTAANI LAEAAFLEGK YVQAFPFFGV ERRGAPVTAF TRIDNKPIRI
KTQIYEPDVV VVLDPSLLDA VDVTAGLKDE GIVIVNTEKS KEEVLEKLKK KPKKLAIVDA
TTIALEILGL PITNTAILGA VAKATGLVKI ESIEEAIKDT FSGELGEKNA RAAREAYEKT
EVFEL
