PORC_THEMA
ID PORC_THEMA Reviewed; 192 AA.
AC O05650;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 4.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Pyruvate synthase subunit PorC;
DE EC=1.2.7.1;
DE AltName: Full=Pyruvate oxidoreductase gamma chain;
DE Short=POR;
DE AltName: Full=Pyruvic-ferredoxin oxidoreductase subunit gamma;
GN Name=porC; Synonyms=porG; OrderedLocusNames=TM_0015;
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 2-20.
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=8550425; DOI=10.1128/jb.178.1.248-257.1996;
RA Kletzin A., Adams M.W.W.;
RT "Molecular and phylogenetic characterization of pyruvate and 2-
RT ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and
RT pyruvate ferredoxin oxidoreductase from Thermotoga maritima.";
RL J. Bacteriol. 178:248-257(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CoA + 2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate = acetyl-CoA
CC + CO2 + H(+) + 2 reduced [2Fe-2S]-[ferredoxin]; Xref=Rhea:RHEA:12765,
CC Rhea:RHEA-COMP:10000, Rhea:RHEA-COMP:10001, ChEBI:CHEBI:15361,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:33737,
CC ChEBI:CHEBI:33738, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=1.2.7.1;
CC -!- SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma
CC chain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD35109.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA59455.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; X85171; CAA59455.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE000512; AAD35109.1; ALT_INIT; Genomic_DNA.
DR PIR; C59427; A72427.
DR RefSeq; NP_227831.1; NC_000853.1.
DR RefSeq; WP_004082456.1; NZ_CP011107.1.
DR PDB; 2RAA; X-ray; 2.12 A; A=1-192.
DR PDBsum; 2RAA; -.
DR AlphaFoldDB; O05650; -.
DR SMR; O05650; -.
DR STRING; 243274.THEMA_04725; -.
DR EnsemblBacteria; AAD35109; AAD35109; TM_0015.
DR KEGG; tma:TM0015; -.
DR eggNOG; COG1014; Bacteria.
DR InParanoid; O05650; -.
DR OMA; PDYVIVQ; -.
DR OrthoDB; 1777574at2; -.
DR BioCyc; MetaCyc:MON-425; -.
DR BRENDA; 1.2.7.1; 6331.
DR EvolutionaryTrace; O05650; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0019164; F:pyruvate synthase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.920.10; -; 1.
DR InterPro; IPR011894; PorC_KorC.
DR InterPro; IPR019752; Pyrv/ketoisovalerate_OxRed_cat.
DR InterPro; IPR002869; Pyrv_flavodox_OxRed_cen.
DR Pfam; PF01558; POR; 1.
DR SUPFAM; SSF53323; SSF53323; 1.
DR TIGRFAMs; TIGR02175; PorC_KorC; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Oxidoreductase;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:8550425"
FT CHAIN 2..192
FT /note="Pyruvate synthase subunit PorC"
FT /id="PRO_0000099917"
FT CONFLICT 23
FT /note="A -> V (in Ref. 1; CAA59455)"
FT /evidence="ECO:0000305"
FT STRAND 7..15
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 20..33
FT /evidence="ECO:0007829|PDB:2RAA"
FT STRAND 38..43
FT /evidence="ECO:0007829|PDB:2RAA"
FT STRAND 52..60
FT /evidence="ECO:0007829|PDB:2RAA"
FT STRAND 74..80
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 86..89
FT /evidence="ECO:0007829|PDB:2RAA"
FT STRAND 96..101
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 106..113
FT /evidence="ECO:0007829|PDB:2RAA"
FT STRAND 117..122
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 124..131
FT /evidence="ECO:0007829|PDB:2RAA"
FT STRAND 132..134
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 138..149
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 154..164
FT /evidence="ECO:0007829|PDB:2RAA"
FT HELIX 171..187
FT /evidence="ECO:0007829|PDB:2RAA"
SQ SEQUENCE 192 AA; 21270 MW; C58296B289254B70 CRC64;
MPVAKKYFEI RWHGRAGQGA KSASQMLAEA ALEAGKYVQA FPEYGAERTG APMRAFNRIG
DEYIRVRSAV ENPDVVVVID ETLLSPAIVE GLSEDGILLV NTVKDFEFVR KKTGFNGKIC
VVDATDIALQ EIKRGIPNTP MLGALVRVTG IVPLEAIEKR IEKMFGKKFP QEVIDANKRA
LRRGYEEVKC SE
