ID   A9_VAR67                Reviewed;          95 AA.
AC   P33835;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=Virion membrane protein A9;
DE   Flags: Precursor;
GN   ORFNames=A9L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1666548;
RA   Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA   Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA   Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT   "Creation of a clone library of fragments from the natural variola virus
RT   and study of the structural and functional organization of viral genes from
RT   a circle of hosts.";
RL   Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Envelope protein. Required for an early step in virion
CC       morphogenesis (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250}; Single-pass
CC       membrane protein {ECO:0000250}. Host cytoplasm {ECO:0000250}.
CC       Note=Component of the mature virion (MV) membrane. The mature virion is
CC       located in the cytoplasm of infected cells and is probably released by
CC       cell lysis. Also found in cytoplasmic virus factories (By similarity).
CC       {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae A9 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; X69198; CAA49054.1; -; Genomic_DNA.
DR   EMBL; X67116; CAA47516.1; -; Genomic_DNA.
DR   PIR; A36849; A36849.
DR   RefSeq; NP_042157.1; NC_001611.1.
DR   SMR; P33835; -.
DR   GeneID; 1486539; -.
DR   KEGG; vg:1486539; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0019031; C:viral envelope; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR006920; Poxvirus_A9.
DR   Pfam; PF04835; Pox_A9; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Host cytoplasm; Late protein; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane helix; Viral envelope protein; Virion.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..95
FT                   /note="Virion membrane protein A9"
FT                   /id="PRO_0000099228"
FT   TOPO_DOM        23..45
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        46..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        67..83
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255"
FT   REGION          76..95
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        79
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        87
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   95 AA;  10667 MW;  37E6E6F7A712FC65 CRC64;
     MSCYTAILKS VGGLALFQDA NGAIDLCRHF FMYFCEQKLR PNSFWFVVVR AIASMIMYLV
     LGIALLYISE QDDKKNTNNA SNSNKLNESS INSNS