PORO_PSEAE
ID PORO_PSEAE Reviewed; 438 AA.
AC P32977;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Porin O;
DE Flags: Precursor;
GN Name=oprO; OrderedLocusNames=PA3280;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / PAO1 / H103;
RX PubMed=1406271; DOI=10.1111/j.1365-2958.1992.tb01407.x;
RA Siehnel R.J., Egli C., Hancock R.E.W.;
RT "Polyphosphate-selective porin OprO of Pseudomonas aeruginosa: expression,
RT purification and sequence.";
RL Mol. Microbiol. 6:2319-2326(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Anion specific, the binding site has higher affinity for
CC phosphate than chloride ions. Porin O has a higher affinity for
CC polyphosphates (tripolyphosphate and pyrophosphate) while porin P has a
CC higher affinity for orthophosphate.
CC -!- SUBCELLULAR LOCATION: Cell outer membrane; Multi-pass membrane protein.
CC -!- DEVELOPMENTAL STAGE: Expressed only during the stationary growth phase.
CC -!- INDUCTION: By phosphate starvation.
CC -!- SIMILARITY: Belongs to the OprO/OprP family. {ECO:0000305}.
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DR EMBL; M86648; AAA25912.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG06668.1; -; Genomic_DNA.
DR PIR; S25260; S25260.
DR RefSeq; NP_251970.1; NC_002516.2.
DR RefSeq; WP_003104423.1; NZ_QZGE01000019.1.
DR PDB; 4RJW; X-ray; 1.52 A; A=25-438.
DR PDB; 4RJX; X-ray; 1.54 A; A=25-438.
DR PDBsum; 4RJW; -.
DR PDBsum; 4RJX; -.
DR AlphaFoldDB; P32977; -.
DR SMR; P32977; -.
DR STRING; 287.DR97_4650; -.
DR TCDB; 1.B.5.1.2; the pseudomonas oprp porin (pop) family.
DR PaxDb; P32977; -.
DR EnsemblBacteria; AAG06668; AAG06668; PA3280.
DR GeneID; 882443; -.
DR KEGG; pae:PA3280; -.
DR PATRIC; fig|208964.12.peg.3430; -.
DR PseudoCAP; PA3280; -.
DR HOGENOM; CLU_031025_4_1_6; -.
DR InParanoid; P32977; -.
DR OMA; NKYMIWR; -.
DR PhylomeDB; P32977; -.
DR BioCyc; PAER208964:G1FZ6-3341-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 2.40.160.10; -; 1.
DR InterPro; IPR023614; Porin_dom_sf.
DR InterPro; IPR010870; Porin_O/P.
DR Pfam; PF07396; Porin_O_P; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell outer membrane; Chloride; Ion transport; Membrane;
KW Porin; Reference proteome; Signal; Stress response; Transmembrane;
KW Transmembrane beta strand; Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..438
FT /note="Porin O"
FT /id="PRO_0000025211"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 34..37
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 39..46
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 52..66
FT /evidence="ECO:0007829|PDB:4RJW"
FT HELIX 68..70
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 71..75
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 77..93
FT /evidence="ECO:0007829|PDB:4RJW"
FT TURN 94..96
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 97..104
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 109..111
FT /evidence="ECO:0007829|PDB:4RJW"
FT TURN 112..114
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 117..124
FT /evidence="ECO:0007829|PDB:4RJW"
FT TURN 126..129
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 130..136
FT /evidence="ECO:0007829|PDB:4RJW"
FT HELIX 143..146
FT /evidence="ECO:0007829|PDB:4RJW"
FT TURN 149..151
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 153..156
FT /evidence="ECO:0007829|PDB:4RJW"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:4RJW"
FT HELIX 165..168
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 174..182
FT /evidence="ECO:0007829|PDB:4RJW"
FT TURN 183..185
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 186..199
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 204..219
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 222..234
FT /evidence="ECO:0007829|PDB:4RJW"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 256..259
FT /evidence="ECO:0007829|PDB:4RJW"
FT HELIX 262..264
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 280..295
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 298..311
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 319..331
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 337..340
FT /evidence="ECO:0007829|PDB:4RJX"
FT TURN 341..344
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 345..349
FT /evidence="ECO:0007829|PDB:4RJX"
FT TURN 354..356
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 358..372
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 380..382
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 387..399
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 401..418
FT /evidence="ECO:0007829|PDB:4RJW"
FT STRAND 425..438
FT /evidence="ECO:0007829|PDB:4RJW"
SQ SEQUENCE 438 AA; 47788 MW; 0997AE6A308AE051 CRC64;
MIRKHSLGFV ASALALAVSA QAFAGTVTTD GADIVIKTKG GLEVATTDKE FSFKLGGRLQ
ADYSRFDGFY TKNGNTADAA YFRRAFIELG GTAYKDWKYQ INFDLSHNTG SSDNGYFDEA
SVTYTGFNPV NLKFGRFDPD FGLEKATSSK WVTAPERNAA YELADWINTH QDGMGAQVNS
TLADMAYLSA GVSAKDADDS DGDSVKQFNF RGVFAPMHEA GNVLHVGVNY AYRDLDDTAF
DSRIRPRLGM RGIATSGGND AGDNGNRATF GGVSNSPAGS YKDDSVWGLE GAWAMGPFSA
QAEYLARKLK ADDNAYKDIK AKGYYAQLAY TLTGESRQYK LEGAKFDSVK PENKEIGAWE
VFYRYDNIKV EDDNVVADTA TREVGDTKAK AHNLGVNWYV NDAVKISAAY VKAKTDKITN
NNGDDDGDGF VTRLQYVF
