AT9_ACTCC
ID AT9_ACTCC Reviewed; 432 AA.
AC A0A2R6PLK2;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 1.
DT 03-AUG-2022, entry version 14.
DE RecName: Full=Alcohol acyltransferase 9 {ECO:0000305};
DE Short=AcAT9 {ECO:0000305};
DE EC=2.3.1.268 {ECO:0000250|UniProtKB:A0A068BIF1};
GN Name=AT9 {ECO:0000305};
GN ORFNames=CEY00_Acc27825 {ECO:0000312|EMBL:PSR93215.1};
OS Actinidia chinensis var. chinensis (Chinese soft-hair kiwi).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=1590841;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Red5; TISSUE=Leaf;
RX PubMed=29661190; DOI=10.1186/s12864-018-4656-3;
RA Pilkington S.M., Crowhurst R., Hilario E., Nardozza S., Fraser L., Peng Y.,
RA Gunaseelan K., Simpson R., Tahir J., Deroles S.C., Templeton K., Luo Z.,
RA Davy M., Cheng C., McNeilage M., Scaglione D., Liu Y., Zhang Q., Datson P.,
RA De Silva N., Gardiner S.E., Bassett H., Chagne D., McCallum J.,
RA Dzierzon H., Deng C., Wang Y.Y., Barron L., Manako K., Bowen J.,
RA Foster T.M., Erridge Z.A., Tiffin H., Waite C.N., Davies K.M.,
RA Grierson E.P., Laing W.A., Kirk R., Chen X., Wood M., Montefiori M.,
RA Brummell D.A., Schwinn K.E., Catanach A., Fullerton C., Li D.,
RA Meiyalaghan S., Nieuwenhuizen N., Read N., Prakash R., Hunter D., Zhang H.,
RA McKenzie M., Knabel M., Harris A., Allan A.C., Gleave A., Chen A.,
RA Janssen B.J., Plunkett B., Ampomah-Dwamena C., Voogd C., Leif D.,
RA Lafferty D., Souleyre E.J.F., Varkonyi-Gasic E., Gambi F., Hanley J.,
RA Yao J.L., Cheung J., David K.M., Warren B., Marsh K., Snowden K.C.,
RA Lin-Wang K., Brian L., Martinez-Sanchez M., Wang M., Ileperuma N.,
RA Macnee N., Campin R., McAtee P., Drummond R.S.M., Espley R.V.,
RA Ireland H.S., Wu R., Atkinson R.G., Karunairetnam S., Bulley S.,
RA Chunkath S., Hanley Z., Storey R., Thrimawithana A.H., Thomson S.,
RA David C., Testolin R., Huang H., Hellens R.P., Schaffer R.J.;
RT "A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome
RT highlights the challenges associated with draft genomes and gene prediction
RT in plants.";
RL BMC Genomics 19:257-257(2018).
CC -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC kiwifruit flavor (By similarity). Alcohol acyl transferase that can use
CC a wide range of alcohols as substrate to produce esters (By
CC similarity). Exhibits acetyl-CoA:alcohol O-acyltransferase activity (By
CC similarity). {ECO:0000250|UniProtKB:A0A068BIF1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(methylsulfanyl)acetyl-CoA + butan-1-ol = butyl 2-
CC (methylsulfanyl)acetate + CoA; Xref=Rhea:RHEA:64672,
CC ChEBI:CHEBI:28885, ChEBI:CHEBI:57287, ChEBI:CHEBI:156076,
CC ChEBI:CHEBI:156077; Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64673;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate;
CC Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55973;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + butan-1-ol = butyl acetate + CoA;
CC Xref=Rhea:RHEA:64632, ChEBI:CHEBI:28885, ChEBI:CHEBI:31328,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64633;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=butan-1-ol + propanoyl-CoA = butyl propanoate + CoA;
CC Xref=Rhea:RHEA:64644, ChEBI:CHEBI:28885, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57392, ChEBI:CHEBI:89831;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64645;
CC Evidence={ECO:0000250|UniProtKB:A0A068BIF1};
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; NKQK01000024; PSR93215.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2R6PLK2; -.
DR SMR; A0A2R6PLK2; -.
DR EnsemblPlants; PSR93215; PSR93215; CEY00_Acc27825.
DR Gramene; PSR93215; PSR93215; CEY00_Acc27825.
DR OMA; DFGMGRP; -.
DR Proteomes; UP000241394; Chromosome lg24.
DR GO; GO:0016746; F:acyltransferase activity; ISS:UniProtKB.
DR GO; GO:0006066; P:alcohol metabolic process; ISS:UniProtKB.
DR GO; GO:0009836; P:fruit ripening, climacteric; ISS:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; ISS:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 3: Inferred from homology;
KW Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..432
FT /note="Alcohol acyltransferase 9"
FT /id="PRO_0000451704"
FT ACT_SITE 156
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT ACT_SITE 379
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
SQ SEQUENCE 432 AA; 47639 MW; F4AB028DF0595194 CRC64;
MASSVRLVKK PVLVAPVDPT PSTVLSLSSL DSQLFLRFPI EYLLVYASPH GVDRDVTAAR
VKAALARSLV PYYPLAGRVK TRPDSTGLDV VCQAQGAGLL EAVSDYTASD FQRAPRSVAE
WRKLLLVEVF KVVPPLVVQL TWLSDGCVAL GIGFSHCVID GIGSSEFLNL FAELATSRAK
LSEFQPKPVW DRQLLNSAGR TNLGTHPEFG RVPDLSGFVT RFAQERLSPT SITFDKTWLK
ELKNIAMSTS QAGEFPYTSF EVLSGHIWRS WARSLNLPAK QVLKLLFSIN IRNRVKPSLP
AGYYGNAFVL GCAQTSVKDL TEKGLGYCAD LVRGAKERVG DEYAREVVES VSWPRRASPD
SVGVLIISQW SRLGLDRVDF GLGRPVHVGP ICCDRYCLFL PVRDRMEAVK VMVAVPTSAV
DRYENLMRSP YS
