AT9_ACTER
ID AT9_ACTER Reviewed; 432 AA.
AC A0A068BIF1;
DT 02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2014, sequence version 1.
DT 03-AUG-2022, entry version 14.
DE RecName: Full=Alcohol acyltransferase 9 {ECO:0000303|PubMed:21450321};
DE Short=AeAT9 {ECO:0000303|PubMed:21450321};
DE EC=2.3.1.268 {ECO:0000269|PubMed:21450321};
GN Name=AT9 {ECO:0000303|PubMed:21450321};
OS Actinidia eriantha (Velvet vine) (Actinidia fulvicoma var. lanata).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Actinidiaceae; Actinidia.
OX NCBI_TaxID=165200;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION BY ETHYLENE.
RX PubMed=21450321; DOI=10.1016/j.phytochem.2011.02.026;
RA Guenther C.S., Chervin C., Marsh K.B., Newcomb R.D., Souleyre E.J.F.;
RT "Characterisation of two alcohol acyltransferases from kiwifruit (Actinidia
RT spp.) reveals distinct substrate preferences.";
RL Phytochemistry 72:700-710(2011).
CC -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC kiwifruit flavor (PubMed:21450321). Alcohol acyl transferase that can
CC use a wide range of alcohols as substrate to produce esters
CC (PubMed:21450321). Exhibits acetyl-CoA:alcohol O-acyltransferase
CC activity (PubMed:21450321). {ECO:0000269|PubMed:21450321}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(methylsulfanyl)acetyl-CoA + butan-1-ol = butyl 2-
CC (methylsulfanyl)acetate + CoA; Xref=Rhea:RHEA:64672,
CC ChEBI:CHEBI:28885, ChEBI:CHEBI:57287, ChEBI:CHEBI:156076,
CC ChEBI:CHEBI:156077; Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64673;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + ethanol = CoA + ethyl acetate;
CC Xref=Rhea:RHEA:55972, ChEBI:CHEBI:16236, ChEBI:CHEBI:27750,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.268;
CC Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:55973;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + butan-1-ol = butyl acetate + CoA;
CC Xref=Rhea:RHEA:64632, ChEBI:CHEBI:28885, ChEBI:CHEBI:31328,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288;
CC Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64633;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=butan-1-ol + propanoyl-CoA = butyl propanoate + CoA;
CC Xref=Rhea:RHEA:64644, ChEBI:CHEBI:28885, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57392, ChEBI:CHEBI:89831;
CC Evidence={ECO:0000269|PubMed:21450321};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:64645;
CC Evidence={ECO:0000269|PubMed:21450321};
CC -!- TISSUE SPECIFICITY: Expressed in fruit. {ECO:0000269|PubMed:21450321}.
CC -!- DEVELOPMENTAL STAGE: Accumulates in kiwifruit during ripening.
CC {ECO:0000269|PubMed:21450321}.
CC -!- INDUCTION: Induced by ethylene in ripe fruits.
CC {ECO:0000269|PubMed:21450321}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; KJ626344; AIC83789.1; -; mRNA.
DR AlphaFoldDB; A0A068BIF1; -.
DR SMR; A0A068BIF1; -.
DR BioCyc; MetaCyc:MON-16564; -.
DR GO; GO:0016746; F:acyltransferase activity; IDA:UniProtKB.
DR GO; GO:0006066; P:alcohol metabolic process; IDA:UniProtKB.
DR GO; GO:0009836; P:fruit ripening, climacteric; IEP:UniProtKB.
DR GO; GO:0009723; P:response to ethylene; IEP:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Transferase.
FT CHAIN 1..432
FT /note="Alcohol acyltransferase 9"
FT /id="PRO_0000451702"
FT ACT_SITE 156
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT ACT_SITE 379
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9FI78"
SQ SEQUENCE 432 AA; 47661 MW; CE616A5FDCE3BEE5 CRC64;
MASSVRLVKK PVLVAPVDPT PSTVLSLSSL DSQLFLRFPI EYLLVYASPH GVDRAVTAAR
VKAALARSLV PYYPLAGRVK TRPDSTGLDV VCQAQGAGLL EAVSDYTASD FQRAPRSVTE
WRKLLLVEVF KVVPPLVVQL TWLSDGCVAL GVGFSHCVID GIGSSEFLNL FAELATGRAR
LSEFQPKPVW DRHLLNSAGR TNLGTHPEFG RVPDLSGFVT RFTQERLSPT SITFDKTWLK
ELKNIAMSTS QPGEFPYTSF EVLSGHIWRS WARSLNLPAK QVLKLLFSIN IRNRVKPSLP
AGYYGNAFVL GCAQTSVKDL TEKGLGYCAD LVRGAKERVG DEYAREVVES VSWPRRASPD
SVGVLIISQW SRLGLDRVDF GLGRPVQVGP ICCDRYCLFL PVRESTESVK VMVAVPTSAV
DRYEYFIRSP YS
