PORTL_BPPZA
ID PORTL_BPPZA Reviewed; 309 AA.
AC P07535;
DT 01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 23-FEB-2022, entry version 77.
DE RecName: Full=Portal protein {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Connector protein {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Gene product 10 {ECO:0000250|UniProtKB:P04332};
DE Short=gp10 {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Gene product 19 {ECO:0000250|UniProtKB:P04332};
DE Short=gp19 {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Head-to-tail connector {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Probable portal protein {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Protein p10 {ECO:0000250|UniProtKB:P04332};
DE AltName: Full=Upper collar protein {ECO:0000250|UniProtKB:P04332};
GN Name=10;
OS Bacillus phage PZA (Bacteriophage PZA).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Salasmaviridae; Picovirinae; Salasvirus; Bacillus virus PZA.
OX NCBI_TaxID=10757;
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3095188; DOI=10.1016/0378-1119(86)90048-x;
RA Paces V., Vlcek C., Urbanek P.;
RT "Nucleotide sequence of the late region of Bacillus subtilis phage PZA, a
RT close relative of phi 29.";
RL Gene 44:107-114(1986).
CC -!- FUNCTION: Forms the portal vertex of the capsid. This portal plays
CC critical roles in head assembly, genome packaging, neck/tail
CC attachment, and genome ejection. The portal protein multimerizes as a
CC single ring-shaped homododecamer arranged around a central channel.
CC Binds to the 6 packaging RNA molecules (pRNA) forming a double-ring
CC structure which in turn binds to the ATPase gp16 hexamer, forming the
CC active DNA-translocating motor. This complex is essential for the
CC specificity of packaging from the left DNA end.
CC {ECO:0000250|UniProtKB:P04332, ECO:0000250|UniProtKB:P13334}.
CC -!- SUBUNIT: Homododecamer. Interacts (via N-terminus) with the pRNA.
CC {ECO:0000250|UniProtKB:P04332}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P04332}.
CC Note=Present in 12 copies in the virion.
CC {ECO:0000250|UniProtKB:P04332}.
CC -!- SIMILARITY: Belongs to the phi29likevirus portal protein family.
CC {ECO:0000305}.
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DR EMBL; M11813; AAA88487.1; -; Genomic_DNA.
DR PIR; E24831; WMBP10.
DR SMR; P07535; -.
DR Proteomes; UP000000855; Genome.
DR GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IEA:UniProtKB-KW.
DR InterPro; IPR008016; Gp10.
DR InterPro; IPR036199; Gp10_sf.
DR Pfam; PF05352; Phage_connector; 1.
DR SUPFAM; SSF56826; SSF56826; 1.
PE 3: Inferred from homology;
KW Capsid protein; Late protein; RNA-binding; Viral capsid assembly;
KW Viral genome ejection through host cell envelope; Viral genome packaging;
KW Viral penetration into host cytoplasm; Viral release from host cell;
KW Viral short tail ejection system; Virion; Virus entry into host cell.
FT CHAIN 1..309
FT /note="Portal protein"
FT /id="PRO_0000106587"
FT REGION 1..14
FT /note="Binding to the pRNA"
FT /evidence="ECO:0000250|UniProtKB:P04332"
SQ SEQUENCE 309 AA; 35865 MW; 0F5E52DC617A9DF7 CRC64;
MGRKRSNSYR SINEIQRQKR NRWFIHYLNY LQSLAYQLFE WENLPPTINP SFLEKSIHQF
GYVGFYKDPV ISYIACNGAL SGQRDVYNQA TVFRASSPVY QKEFKLYNYR DMKEDDMGVV
IYNNDMSFPT TPTLELFAAE LAELKEIISV NQNAQKTPVL IRANDNNQLS LKQVYNQYEG
NAPVIFAHEA LDSDSIEVFK TDAPYVVDKL NAQKNAVWNE MMTFLGIKNA NLEKKERMVT
DEVSSNDEQI ESSGTVFLKS REEACEKINE LYGLDVKVRF RYDIVEQMRR ELQQIENVSR
GTLGGETNE
