ID   PORTL_BPT5              Reviewed;         403 AA.
AC   Q6QGD5;
DT   01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Portal protein {ECO:0000303|PubMed:24198424};
DE   AltName: Full=Capsid protein pb7 {ECO:0000303|PubMed:24198424};
DE   Flags: Precursor;
GN   ORFNames=ORF141, T5.152 {ECO:0000312|EMBL:AAS77191.1},
GN   T5p148 {ECO:0000312|EMBL:AAU05287.1};
OS   Escherichia phage T5 (Enterobacteria phage T5).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Demerecviridae; Markadamsvirinae; Tequintavirus.
OX   NCBI_TaxID=2695836;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Ksenzenko V.N., Kaliman A.V., Krutilina A.I., Shlyapnikov M.G.;
RT   "Bacteriophage T5 complete genome.";
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 11303-B5 {ECO:0000312|EMBL:AAX12078.1};
RX   PubMed=15661140; DOI=10.1016/j.virol.2004.10.049;
RA   Wang J., Jiang Y., Vincent M., Sun Y., Yu H., Wang J., Bao Q., Kong H.,
RA   Hu S.;
RT   "Complete genome sequence of bacteriophage T5.";
RL   Virology 332:45-65(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], PARTIAL PROTEIN SEQUENCE,
RP   PROTEOLYTIC CLEAVAGE, AND SUBCELLULAR LOCATION.
RC   STRAIN=St0 deletion mutant {ECO:0000312|EMBL:AAU05287.1};
RX   PubMed=24198424; DOI=10.1128/jvi.02262-13;
RA   Zivanovic Y., Confalonieri F., Ponchon L., Lurz R., Chami M., Flayhan A.,
RA   Renouard M., Huet A., Decottignies P., Davidson A.R., Breyton C.,
RA   Boulanger P.;
RT   "Insights into bacteriophage T5 structure from analysis of its
RT   morphogenesis genes and protein components.";
RL   J. Virol. 88:1162-1174(2014).
RN   [4]
RP   STRUCTURE BY ELECTRON MICROSCOPY (30.0 ANGSTROMS) OF THE CAPSID, FUNCTION,
RP   AND INTERACTION WITH THE MAJOR CAPSID PROTEIN.
RX   PubMed=26616586; DOI=10.1016/j.jmb.2015.11.019;
RA   Huet A., Duda R.L., Hendrix R.W., Boulanger P., Conway J.F.;
RT   "Correct assembly of the bacteriophage T5 procapsid requires both the
RT   maturation protease and the portal complex.";
RL   J. Mol. Biol. 428:165-181(2016).
CC   -!- FUNCTION: Forms the portal vertex of the capsid and plays a role in
CC       governing correct capsid geometry (PubMed:26616586). This portal plays
CC       critical roles in capsid assembly, genome packaging, head completion
CC       protein attachment, and genome ejection (By similarity). The portal
CC       protein multimerizes as a single ring-shaped homododecamer arranged
CC       around a central channel (By similarity). Binds to the terminase
CC       subunits to form the packaging machine (By similarity).
CC       {ECO:0000250|UniProtKB:P25480, ECO:0000269|PubMed:26616586}.
CC   -!- SUBUNIT: Homododecamer (By similarity). Interacts with the major capsid
CC       protein (Probable). {ECO:0000250|UniProtKB:P25480,
CC       ECO:0000269|PubMed:26616586}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:24198424}. Note=Forms
CC       the portal complex located at one vertex of the capsid.
CC       {ECO:0000269|PubMed:24198424}.
CC   -!- SIMILARITY: Belongs to the phage portal family. {ECO:0000305}.
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DR   EMBL; AY543070; AAS77191.1; -; Genomic_DNA.
DR   EMBL; AY692264; AAU05287.1; -; Genomic_DNA.
DR   EMBL; AY587007; AAX12078.1; -; Genomic_DNA.
DR   RefSeq; YP_006980.1; NC_005859.1.
DR   TCDB; 1.W.2.1.1; the phage portal protein 2 (ppp2) family.
DR   GeneID; 2777676; -.
DR   KEGG; vg:2777676; -.
DR   Proteomes; UP000002107; Genome.
DR   Proteomes; UP000002141; Genome.
DR   Proteomes; UP000002503; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:GOC.
DR   GO; GO:0019028; C:viral capsid; IEA:UniProtKB-KW.
DR   GO; GO:0039710; P:cytoplasmic icosahedral capsid assembly; IDA:CACAO.
DR   GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR   InterPro; IPR006944; Phage/GTA_portal.
DR   Pfam; PF04860; Phage_portal; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Direct protein sequencing; Late protein;
KW   Reference proteome; Viral capsid assembly;
KW   Viral genome ejection through host cell envelope; Viral genome packaging;
KW   Viral long flexible tail ejection system;
KW   Viral penetration into host cytoplasm; Viral release from host cell;
KW   Virion; Virus entry into host cell.
FT   PROPEP          1..10
FT                   /evidence="ECO:0000269|PubMed:24198424"
FT                   /id="PRO_0000435560"
FT   CHAIN           11..403
FT                   /note="Portal protein"
FT                   /id="PRO_0000432540"
FT   REGION          379..403
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            10..11
FT                   /note="Cleavage; by the prohead protease"
FT                   /evidence="ECO:0000269|PubMed:24198424"
SQ   SEQUENCE   403 AA;  45087 MW;  D1F254D0AF0EB9DB CRC64;
     MGFKSWITEK LNPGQRIIRD MEPVSHRTNR KPFTTGQAYS KIEILNRTAN MVIDSAAECS
     YTVGDKYNIV TYANGVKTKT LDTLLNVRPN PFMDISTFRR LVVTDLLFEG CAYIYWDGTS
     LYHVPAALMQ VEADANKFIK KFIFNNQINY RVDEIIFIKD NSYVCGTNSQ ISGQSRVATV
     IDSLEKRSKM LNFKEKFLDN GTVIGLILET DEILNKKLRE RKQEELQLDY NPSTGQSSVL
     ILDGGMKAKP YSQISSFKDL DFKEDIEGFN KSICLAFGVP QVLLDGGNNA NIRPNIELFY
     YMTIIPMLNK LTSSLTFFFG YKITPNTKEV AALTPDKEAE AKHLTSLVNN GIITGNEARS
     ELNLEPLDDE QMNKIRIPAN VAGSATGVSG QEGGRPKGST EGD