PORTL_LAMBD
ID PORTL_LAMBD Reviewed; 533 AA.
AC P03710;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 21-JUL-1986, sequence version 1.
DT 02-DEC-2020, entry version 92.
DE RecName: Full=Portal protein B {ECO:0000255|HAMAP-Rule:MF_04135};
DE AltName: Full=GpB {ECO:0000255|HAMAP-Rule:MF_04135};
DE AltName: Full=Minor capsid protein B {ECO:0000255|HAMAP-Rule:MF_04135};
DE Contains:
DE RecName: Full=Protein B* {ECO:0000255|HAMAP-Rule:MF_04135};
GN Name=B {ECO:0000255|HAMAP-Rule:MF_04135}; OrderedLocusNames=lambdap04;
OS Escherichia phage lambda (Bacteriophage lambda).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Lambdavirus.
OX NCBI_TaxID=10710;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=6221115; DOI=10.1016/0022-2836(82)90546-0;
RA Sanger F., Coulson A.R., Hong G.F., Hill D.F., Petersen G.B.;
RT "Nucleotide sequence of bacteriophage lambda DNA.";
RL J. Mol. Biol. 162:729-773(1982).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=6228056; DOI=10.1016/0042-6822(83)90537-8;
RA Kochan J., Murialdo H.;
RT "Early intermediates in bacteriophage lambda prohead assembly. II.
RT Identification of biologically active intermediates.";
RL Virology 131:100-115(1983).
RN [3]
RP FUNCTION, AND SUBUNIT.
RX PubMed=6232391; DOI=10.1016/0022-2836(84)90330-9;
RA Kochan J., Carrascosa J.L., Murialdo H.;
RT "Bacteriophage lambda preconnectors. Purification and structure.";
RL J. Mol. Biol. 174:433-447(1984).
RN [4]
RP INTERACTION WITH TERMINASE COMPLEX.
RX PubMed=7799432; DOI=10.1006/jmbi.1994.0013;
RA Yeo A., Feiss M.;
RT "Specific interaction of terminase, the DNA packaging enzyme of
RT bacteriophage lambda, with the portal protein of the prohead.";
RL J. Mol. Biol. 245:141-150(1995).
RN [5]
RP PROTEOLYTIC CLEAVAGE.
RX PubMed=20620152; DOI=10.1016/j.jmb.2010.06.060;
RA Medina E., Wieczorek D., Medina E.M., Yang Q., Feiss M., Catalano C.E.;
RT "Assembly and maturation of the bacteriophage lambda procapsid: gpC is the
RT viral protease.";
RL J. Mol. Biol. 401:813-830(2010).
CC -!- FUNCTION: Forms the portal vertex of the capsid (PubMed:6228056,
CC PubMed:6232391). This portal plays critical roles in head assembly,
CC genome packaging, neck/tail attachment, and genome ejection (Probable).
CC The portal protein multimerizes as a single ring-shaped homododecamer
CC arranged around a central channel (PubMed:6232391). Binds to the
CC terminase subunits to form the packaging machine (PubMed:7799432).
CC {ECO:0000255|HAMAP-Rule:MF_04135, ECO:0000269|PubMed:6228056,
CC ECO:0000269|PubMed:6232391, ECO:0000269|PubMed:7799432, ECO:0000305}.
CC -!- SUBUNIT: Homododecamer (PubMed:6232391). Interacts with the terminase
CC complex composed of two small and one large terminase subunits
CC (PubMed:7799432). {ECO:0000269|PubMed:6232391,
CC ECO:0000269|PubMed:7799432}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04135,
CC ECO:0000269|PubMed:6228056}.
CC -!- PTM: Proteolytically cleaved by the viral protease during capsid
CC maturation. {ECO:0000255|HAMAP-Rule:MF_04135,
CC ECO:0000269|PubMed:20620152}.
CC -!- SIMILARITY: Belongs to the siphoviridae portal protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04135}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-15 is the initiator.
CC {ECO:0000305}.
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DR EMBL; J02459; AAA96536.1; -; Genomic_DNA.
DR PIR; F04333; VHBPBL.
DR RefSeq; NP_040583.1; NC_001416.1.
DR TCDB; 1.W.5.1.1; the (lambda) phage portal protein 5 (ppp5) family.
DR GeneID; 2703526; -.
DR KEGG; vg:2703526; -.
DR Proteomes; UP000001711; Genome.
DR GO; GO:0046798; C:viral portal complex; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; IEA:UniProtKB-UniRule.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-UniRule.
DR GO; GO:0019068; P:virion assembly; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04135; PORTAL_LAMBDA; 1.
DR InterPro; IPR006429; Phage_lambda_portal.
DR Pfam; PF05136; Phage_portal_2; 1.
DR TIGRFAMs; TIGR01539; portal_lambda; 1.
PE 1: Evidence at protein level;
KW Capsid protein; DNA-binding; Reference proteome; Viral capsid assembly;
KW Viral genome ejection through host cell envelope; Viral genome packaging;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral release from host cell;
KW Virion; Virus entry into host cell.
FT CHAIN 1..533
FT /note="Portal protein B"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04135"
FT /id="PRO_0000003372"
FT CHAIN 23..533
FT /note="Protein B*"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04135"
FT /id="PRO_0000003373"
FT SITE 22..23
FT /note="Cleavage; by viral protease"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04135,
FT ECO:0000269|PubMed:20620152"
SQ SEQUENCE 533 AA; 59466 MW; 4E8E25B30A9144A0 CRC64;
MKTPTIPTLL GPDGMTSLRE YAGYHGGGSG FGGQLRSWNP PSESVDAALL PNFTRGNARA
DDLVRNNGYA ANAIQLHQDH IVGSFFRLSH RPSWRYLGIG EEEARAFSRE VEAAWKEFAE
DDCCCIDVER KRTFTMMIRE GVAMHAFNGE LFVQATWDTS SSRLFRTQFR MVSPKRISNP
NNTGDSRNCR AGVQINDSGA ALGYYVSEDG YPGWMPQKWT WIPRELPGGR ASFIHVFEPV
EDGQTRGANV FYSVMEQMKM LDTLQNTQLQ SAIVKAMYAA TIESELDTQS AMDFILGANS
QEQRERLTGW IGEIAAYYAA APVRLGGAKV PHLMPGDSLN LQTAQDTDNG YSVFEQSLLR
YIAAGLGVSY EQLSRNYAQM SYSTARASAN ESWAYFMGRR KFVASRQASQ MFLCWLEEAI
VRRVVTLPSK ARFSFQEARS AWGNCDWIGS GRMAIDGLKE VQEAVMLIEA GLSTYEKECA
KRGDDYQEIF AQQVRETMER RAAGLKPPAW AAAAFESGLR QSTEEEKSDS RAA
