POR_SYNY3
ID POR_SYNY3 Reviewed; 322 AA.
AC Q59987; Q55825;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 25-MAY-2022, entry version 138.
DE RecName: Full=Light-dependent protochlorophyllide reductase;
DE Short=PCR;
DE EC=1.3.1.33;
DE AltName: Full=NADPH-protochlorophyllide oxidoreductase;
DE Short=LPOR;
DE Short=POR;
GN Name=por; Synonyms=pcr; OrderedLocusNames=slr0506;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7731978; DOI=10.1073/pnas.92.9.3749;
RA Suzuki J.Y., Bauer C.E.;
RT "A prokaryotic origin for light-dependent chlorophyll biosynthesis of
RT plants.";
RL Proc. Natl. Acad. Sci. U.S.A. 92:3749-3753(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27184 / PCC 6803 / N-1;
RX PubMed=8590279; DOI=10.1093/dnares/2.4.153;
RA Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N.,
RA Sugiura M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region
RT from map positions 64% to 92% of the genome.";
RL DNA Res. 2:153-166(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
CC -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC chlorophyllide (Chlide).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=chlorophyllide a + NADP(+) = H(+) + NADPH +
CC protochlorophyllide a; Xref=Rhea:RHEA:11132, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83348,
CC ChEBI:CHEBI:83350; EC=1.3.1.33;
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. POR subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA68281.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; L37783; AAA68281.1; ALT_INIT; Genomic_DNA.
DR EMBL; BA000022; BAA10580.1; -; Genomic_DNA.
DR PIR; S76636; S76636.
DR PDB; 6L1G; X-ray; 2.20 A; A/B=1-322.
DR PDB; 6R48; X-ray; 1.87 A; A/B=5-322.
DR PDBsum; 6L1G; -.
DR PDBsum; 6R48; -.
DR AlphaFoldDB; Q59987; -.
DR SMR; Q59987; -.
DR IntAct; Q59987; 4.
DR STRING; 1148.1001743; -.
DR PaxDb; Q59987; -.
DR PRIDE; Q59987; -.
DR EnsemblBacteria; BAA10580; BAA10580; BAA10580.
DR KEGG; syn:slr0506; -.
DR eggNOG; COG1028; Bacteria.
DR InParanoid; Q59987; -.
DR OMA; WSWGNRQ; -.
DR PhylomeDB; Q59987; -.
DR BRENDA; 1.3.1.33; 382.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR005979; Prochl_reduct.
DR InterPro; IPR002347; SDR_fam.
DR PANTHER; PTHR44419; PTHR44419; 1.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR SUPFAM; SSF51735; SSF51735; 1.
DR TIGRFAMs; TIGR01289; LPOR; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chlorophyll biosynthesis; NADP; Oxidoreductase;
KW Photosynthesis; Reference proteome.
FT CHAIN 1..322
FT /note="Light-dependent protochlorophyllide reductase"
FT /id="PRO_0000219917"
FT CONFLICT 23
FT /note="A -> R (in Ref. 1; AAA68281)"
FT /evidence="ECO:0000305"
FT STRAND 8..12
FT /evidence="ECO:0007829|PDB:6R48"
FT TURN 13..15
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 17..28
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 32..38
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 40..49
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 54..56
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 67..79
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 84..89
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 107..109
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 110..115
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 117..132
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 150..153
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 169..172
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 189..215
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 218..223
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 231..243
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 249..251
FT /evidence="ECO:0007829|PDB:6L1G"
FT HELIX 258..270
FT /evidence="ECO:0007829|PDB:6R48"
FT HELIX 272..274
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 277..282
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 290..293
FT /evidence="ECO:0007829|PDB:6R48"
FT STRAND 295..298
FT /evidence="ECO:0007829|PDB:6L1G"
FT HELIX 304..318
FT /evidence="ECO:0007829|PDB:6R48"
SQ SEQUENCE 322 AA; 36062 MW; B805C95E139EA213 CRC64;
MEQPMKPTVI ITGASSGVGL YGAKALIDKG WHVIMACRNL DKTQKVADEL GFPKDSYTII
KLDLGYLDSV RRFVAQFREL GRPLKALVCN AAVYFPLLDE PLWSADDYEL SVATNHLGHF
LLCNLLLEDL KACPDADKRL IILGTVTANS KELGGKIPIP APPDLGNFEG FEAGFKKPIA
MINNKKFKSG KAYKDSKLCN MLTTRELHRR FHQETGIVFN SLYPGCVADT PLFRNHYSLF
RTIFPWFQKN VTKGYVSQEL AGERVAMVVA DDKFKDSGVH WSWGNRQQAG REAFVQELSE
QGSDAQKAQR MWDLSEKLVG LV
