POT1_ARATH
ID POT1_ARATH Reviewed; 712 AA.
AC O22397; O50043;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Potassium transporter 1;
DE Short=AtKT1;
DE Short=AtKUP1;
DE Short=AtPOT1;
GN Name=POT1; Synonyms=KT1, KUP1; OrderedLocusNames=At2g30070;
GN ORFNames=F23F1.18, T27E13.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), AND CHARACTERIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=9350997; DOI=10.1016/s0014-5793(97)01125-3;
RA Quintero F.J., Blatt M.R.;
RT "A new family of K+ transporters from Arabidopsis that are conserved across
RT phyla.";
RL FEBS Lett. 415:206-211(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=9477571; DOI=10.2307/3870628;
RA Kim E.J., Kwak J.M., Uozumi N., Schroeder J.I.;
RT "AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport
RT activity.";
RL Plant Cell 10:51-62(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND CHARACTERIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=9477572; DOI=10.2307/3870629;
RA Fu H.-H., Luan S.;
RT "AtKUP1: a dual-affinity K+ transporter from Arabidopsis.";
RL Plant Cell 10:63-73(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: High-affinity potassium transporter that could play a major
CC role in the uptake of potassium from the rhizosphere. May act as a low-
CC affinity potassium transporter under high potassium concentrations.
CC Could also transport rubidium. {ECO:0000269|PubMed:9477571}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O22397-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O22397-2; Sequence=VSP_008984;
CC -!- TISSUE SPECIFICITY: Detected in the whole mature plant but
CC preferentially expressed in roots and stems, and in potassium-starved
CC plants. {ECO:0000269|PubMed:9477571}.
CC -!- MISCELLANEOUS: Low-affinity activity is strongly inhibited by barium or
CC cesium.
CC -!- SIMILARITY: Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.
CC {ECO:0000305}.
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DR EMBL; AF012656; AAC49844.1; -; Genomic_DNA.
DR EMBL; AF029876; AAB88901.1; -; mRNA.
DR EMBL; AF033118; AAB87687.1; -; mRNA.
DR EMBL; AC004165; AAC16965.1; -; Genomic_DNA.
DR EMBL; AC004680; AAM14984.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08341.1; -; Genomic_DNA.
DR PIR; T00591; T02479.
DR RefSeq; NP_180568.1; NM_128562.4. [O22397-1]
DR AlphaFoldDB; O22397; -.
DR BioGRID; 2907; 1.
DR IntAct; O22397; 1.
DR STRING; 3702.AT2G30070.1; -.
DR TCDB; 2.A.72.3.1; the k(+) uptake permease (kup) family.
DR iPTMnet; O22397; -.
DR PaxDb; O22397; -.
DR PRIDE; O22397; -.
DR ProteomicsDB; 249398; -. [O22397-1]
DR EnsemblPlants; AT2G30070.1; AT2G30070.1; AT2G30070. [O22397-1]
DR GeneID; 817558; -.
DR Gramene; AT2G30070.1; AT2G30070.1; AT2G30070. [O22397-1]
DR KEGG; ath:AT2G30070; -.
DR Araport; AT2G30070; -.
DR TAIR; locus:2045639; AT2G30070.
DR eggNOG; ENOG502QPSA; Eukaryota.
DR HOGENOM; CLU_008142_2_0_1; -.
DR InParanoid; O22397; -.
DR OMA; GHETIIP; -.
DR PhylomeDB; O22397; -.
DR BioCyc; ARA:AT2G30070-MON; -.
DR BioCyc; MetaCyc:MON-14583; -.
DR PRO; PR:O22397; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22397; baseline and differential.
DR Genevisible; O22397; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0006813; P:potassium ion transport; IBA:GO_Central.
DR InterPro; IPR003855; K+_transporter.
DR PANTHER; PTHR30540; PTHR30540; 1.
DR Pfam; PF02705; K_trans; 1.
DR TIGRFAMs; TIGR00794; kup; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..712
FT /note="Potassium transporter 1"
FT /id="PRO_0000209077"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 41..67
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..267
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 289..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..340
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..392
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..424
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..449
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..470
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 471..474
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 496..712
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 16..31
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_008984"
FT CONFLICT 100
FT /note="F -> L (in Ref. 1; AAC49844)"
FT /evidence="ECO:0000305"
FT CONFLICT 180
FT /note="L -> P (in Ref. 1; AAC49844)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="A -> V (in Ref. 1; AAC49844)"
FT /evidence="ECO:0000305"
FT CONFLICT 624
FT /note="E -> G (in Ref. 1; AAC49844)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 712 AA; 79127 MW; 0FE32EE88807536D CRC64;
MNQSPSLIEQ GISQQHLKTL SCANVLTLAY QSLGVIYGDL STSPLYVYKT TFSGKLSLHE
DDEEIFGVFS FIFWTFTLIA LFKYVFIVLS ADDNGEGGTF ALYSLLCRYA KLSILPNHQE
MDEKLSTYAT GSPGETRQSA AVKSFFEKHP KSQKCLLLFV LLGTCMAIGD SVLTPTISVL
SAVSGVKLKI PNLHENYVVI IACIILVAIF SVQRYGTHRV AFIFAPISTA WLLSISSIGV
YNTIKWNPRI VSALSPVYMY KFLRSTGVEG WVSLGGVVLS ITGVETMFAD LGHFSSLSIK
VAFSFFVYPC LILAYMGEAA FLSKHHEDIQ QSFYKAIPEP VFWPVFIVAT FAAVVGSQAV
ISATFSIISQ CCALDCFPRV KIIHTSSKIH GQIYIPEVNW MLMCLCLAVT IGLRDTNMMG
HAYGLAVTSV MLVTTCLMTL VMTIVWKQRI ITVLAFVVFF GSIELLYFSS CVYKVPEGGW
IPILLSLTFM AVMYIWNYGT TKKHEFDVEN KVSMDRIVSL GPSIGMVRVP GIGLVYSNLV
TGVPAVFGHF VTNLPAFHKI LVFVCVKSVQ VPYVGEEERF VISRVGPKEY GMFRSVVRYG
YRDVPREMYD FESRLVSAIV EFVETEPGLE EEEMSSVRRK KEECMEIMEA KEAGVAYILG
HSYAKAKQSS SVLKKLAVNV VFAFMSTNCR GTDVVLNVPH TSLLEVGMVY YV
