POT1_CAEEL
ID POT1_CAEEL Reviewed; 400 AA.
AC P42001; A0A2K5ATS5; A0A2K5ATS9; A0A2K5ATU9;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2019, sequence version 3.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Protection of telomeres homolog 1;
GN Name=pot-1 {ECO:0000312|WormBase:B0280.10a};
GN Synonyms=CeOB2 {ECO:0000303|PubMed:18329362};
GN ORFNames=B0280.10 {ECO:0000312|WormBase:B0280.10a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18329362; DOI=10.1016/j.cell.2007.12.039;
RA Raices M., Verdun R.E., Compton S.A., Haggblom C.I., Griffith J.D.,
RA Dillin A., Karlseder J.;
RT "C. elegans telomeres contain G-strand and C-strand overhangs that are
RT bound by distinct proteins.";
RL Cell 132:745-757(2008).
RN [3]
RP FUNCTION.
RX PubMed=22547822; DOI=10.1073/pnas.1119191109;
RA Cheng C., Shtessel L., Brady M.M., Ahmed S.;
RT "Caenorhabditis elegans POT-2 telomere protein represses a mode of
RT alternative lengthening of telomeres with normal telomere lengths.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:7805-7810(2012).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=23390606; DOI=10.1534/g3.112.004440;
RA Shtessel L., Lowden M.R., Cheng C., Simon M., Wang K., Ahmed S.;
RT "Caenorhabditis elegans POT-1 and POT-2 repress telomere maintenance
RT pathways.";
RL G3 (Bethesda) 3:305-313(2013).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=24297748; DOI=10.1083/jcb.201307181;
RA Ferreira H.C., Towbin B.D., Jegou T., Gasser S.M.;
RT "The shelterin protein POT-1 anchors Caenorhabditis elegans telomeres
RT through SUN-1 at the nuclear periphery.";
RL J. Cell Biol. 203:727-735(2013).
CC -!- FUNCTION: Telomeric DNA-binding protein, which binds to single-stranded
CC C-rich repeat sequences, with high specificity to the 5'-GCCTAA-3'
CC sequence (PubMed:18329362, PubMed:23390606). Repeat sequence binding
CC can be at the 5' or 3' telomeric end (PubMed:18329362). May have a role
CC in protecting the 5' end of the C-rich strand of the telomere
CC (PubMed:23390606). Acts redundantly with pot-2 to negatively regulate
CC telomerase-mediated telomere extension (PubMed:18329362,
CC PubMed:23390606, PubMed:24297748). Also regulates telomere length by
CC the telomerase-independent telomere maintenance pathway called ALT
CC (alternative lengthening of telomeres) (PubMed:23390606,
CC PubMed:22547822, PubMed:24297748). Through sun-1, anchors telomeres to
CC the nuclear envelope in embryos (PubMed:24297748).
CC {ECO:0000269|PubMed:18329362, ECO:0000269|PubMed:22547822,
CC ECO:0000269|PubMed:23390606, ECO:0000269|PubMed:24297748}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:23390606,
CC ECO:0000269|PubMed:24297748, ECO:0000305|PubMed:18329362}. Nucleus
CC envelope {ECO:0000269|PubMed:24297748}. Chromosome, telomere
CC {ECO:0000269|PubMed:23390606, ECO:0000305|PubMed:18329362}. Note=More
CC highly expressed in meiotic pachytene nuclei than in diakinesis-stage
CC oocyte nuclei (PubMed:23390606). Also expressed in mitotic nuclei
CC (PubMed:23390606). {ECO:0000269|PubMed:23390606}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=a {ECO:0000312|WormBase:B0280.10a};
CC IsoId=P42001-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:B0280.10b};
CC IsoId=P42001-2; Sequence=VSP_060412;
CC Name=c {ECO:0000312|WormBase:B0280.10c};
CC IsoId=P42001-3; Sequence=VSP_060413;
CC -!- TISSUE SPECIFICITY: Expressed in sperm and oocytes.
CC {ECO:0000269|PubMed:23390606}.
CC -!- DEVELOPMENTAL STAGE: Expressed in embryos.
CC {ECO:0000269|PubMed:24297748}.
CC -!- SIMILARITY: Belongs to the telombin family. {ECO:0000305}.
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DR EMBL; BX284603; SPC47519.1; -; Genomic_DNA.
DR EMBL; BX284603; SPC47520.1; -; Genomic_DNA.
DR EMBL; BX284603; SPC47521.1; -; Genomic_DNA.
DR PIR; T15304; T15304.
DR AlphaFoldDB; P42001; -.
DR SMR; P42001; -.
DR BioGRID; 46760; 1.
DR STRING; 6239.B0280.10; -.
DR EPD; P42001; -.
DR PaxDb; P42001; -.
DR PeptideAtlas; P42001; -.
DR EnsemblMetazoa; B0280.10a.1; B0280.10a.1; WBGene00015105. [P42001-1]
DR EnsemblMetazoa; B0280.10b.1; B0280.10b.1; WBGene00015105. [P42001-2]
DR EnsemblMetazoa; B0280.10b.2; B0280.10b.2; WBGene00015105. [P42001-2]
DR EnsemblMetazoa; B0280.10b.3; B0280.10b.3; WBGene00015105. [P42001-2]
DR EnsemblMetazoa; B0280.10c.1; B0280.10c.1; WBGene00015105. [P42001-3]
DR UCSC; B0280.10; c. elegans. [P42001-1]
DR WormBase; B0280.10a; CE52581; WBGene00015105; pot-1. [P42001-1]
DR WormBase; B0280.10b; CE52643; WBGene00015105; pot-1. [P42001-2]
DR WormBase; B0280.10c; CE52474; WBGene00015105; pot-1. [P42001-3]
DR eggNOG; ENOG502TINA; Eukaryota.
DR HOGENOM; CLU_804703_0_0_1; -.
DR InParanoid; P42001; -.
DR OrthoDB; 1612114at2759; -.
DR PRO; PR:P42001; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00015105; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000781; C:chromosome, telomeric region; IDA:WormBase.
DR GO; GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell.
DR GO; GO:0043047; F:single-stranded telomeric DNA binding; ISS:WormBase.
DR GO; GO:1904357; P:negative regulation of telomere maintenance via telomere lengthening; IMP:WormBase.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chromosome; DNA-binding; Nucleus; Reference proteome;
KW Telomere.
FT CHAIN 1..400
FT /note="Protection of telomeres homolog 1"
FT /id="PRO_0000448582"
FT VAR_SEQ 1..247
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060412"
FT VAR_SEQ 40..60
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060413"
SQ SEQUENCE 400 AA; 46492 MW; E07898D61D90262E CRC64;
MQYTYQHIQD LVPGPTPQNF YGKIIFIKKK INQIVVLIKD ETQSIYLRVI PKEDQELEFQ
LRQVVRVHRC KIQSILNSKE GIAQIGLFGC HLIAWSQSGK VDNPVIISSR SWTKSDEDSE
RLQTLRKLGK SRRKSGRKTS VDTMANKLIE RREAMFADTF IKSLFNKIAL SRKEHLSRNA
RELFYHRPGD IVETQNLLEI DDSWFNDENS EQFVQYVLNC TTCHVEYNHV EYAQNNIPTN
CRFCQEAMES FHAAFRIRIS IETYGVFLTI PLELIKTELD ICEDWDSESN IVEEEEKVTR
FKKNIQEKVR DASIVHIKGI SSLLLIIMLN INSISLVNNI TENKRILLQK SNVPSSLQLK
ILITPFVIVV VRFFGITWIY SGSSCIEEVL NLIDNCSRRR
