POT2_ARATH
ID POT2_ARATH Reviewed; 794 AA.
AC O22881; O22398;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Potassium transporter 2;
DE Short=AtKT2;
DE Short=AtKUP2;
DE Short=AtPOT2;
GN Name=POT2; Synonyms=KT2, KUP2, SHY3; OrderedLocusNames=At2g40540;
GN ORFNames=T2P4.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=9350997; DOI=10.1016/s0014-5793(97)01125-3;
RA Quintero F.J., Blatt M.R.;
RT "A new family of K+ transporters from Arabidopsis that are conserved across
RT phyla.";
RL FEBS Lett. 415:206-211(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=9477571; DOI=10.2307/3870628;
RA Kim E.J., Kwak J.M., Uozumi N., Schroeder J.I.;
RT "AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport
RT activity.";
RL Plant Cell 10:51-62(1998).
RN [6]
RP FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF ASP-167; PRO-395; GLY-419 AND
RP LEU-560, AND DISRUPTION PHENOTYPE.
RX PubMed=11826303; DOI=10.1105/tpc.010322;
RA Elumalai R.P., Nagpal P., Reed J.W.;
RT "A mutation in the Arabidopsis KT2/KUP2 potassium transporter gene affects
RT shoot cell expansion.";
RL Plant Cell 14:119-131(2002).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
CC -!- FUNCTION: Low-affinity potassium transporter. Could mediate the
CC potassium-dependent cell expansion in growing tissues.
CC {ECO:0000269|PubMed:11826303}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Slightly detected in roots, stems, leaves and
CC flowers of mature plants and in potassium-starved plants.
CC {ECO:0000269|PubMed:9477571}.
CC -!- DEVELOPMENTAL STAGE: Localized to cotyledons and hypocotyl in the early
CC stages of development, and later appears in young leaves.
CC {ECO:0000269|PubMed:11826303}.
CC -!- DISRUPTION PHENOTYPE: Plants display a short hypocotyl phenotype. Kup2-
CC 2, kup2-3 and kup2-7 are considered as null mutants.
CC {ECO:0000269|PubMed:11826303}.
CC -!- SIMILARITY: Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.
CC {ECO:0000305}.
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DR EMBL; AF012657; AAC49845.1; -; mRNA.
DR EMBL; AC002336; AAB87583.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC09845.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09846.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61891.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61892.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61893.1; -; Genomic_DNA.
DR EMBL; BT004049; AAO42081.1; -; mRNA.
DR EMBL; BT005048; AAO50581.1; -; mRNA.
DR PIR; G84830; G84830.
DR RefSeq; NP_001078032.1; NM_001084563.2.
DR RefSeq; NP_001318392.1; NM_001336844.1.
DR RefSeq; NP_001324081.1; NM_001336845.1.
DR RefSeq; NP_001324082.1; NM_001336846.1.
DR RefSeq; NP_565936.1; NM_129616.4.
DR AlphaFoldDB; O22881; -.
DR BioGRID; 3987; 10.
DR IntAct; O22881; 10.
DR STRING; 3702.AT2G40540.1; -.
DR TCDB; 2.A.72.3.8; the k(+) uptake permease (kup) family.
DR iPTMnet; O22881; -.
DR SwissPalm; O22881; -.
DR PaxDb; O22881; -.
DR PRIDE; O22881; -.
DR ProteomicsDB; 249053; -.
DR EnsemblPlants; AT2G40540.1; AT2G40540.1; AT2G40540.
DR EnsemblPlants; AT2G40540.2; AT2G40540.2; AT2G40540.
DR EnsemblPlants; AT2G40540.3; AT2G40540.3; AT2G40540.
DR EnsemblPlants; AT2G40540.4; AT2G40540.4; AT2G40540.
DR EnsemblPlants; AT2G40540.5; AT2G40540.5; AT2G40540.
DR GeneID; 818649; -.
DR Gramene; AT2G40540.1; AT2G40540.1; AT2G40540.
DR Gramene; AT2G40540.2; AT2G40540.2; AT2G40540.
DR Gramene; AT2G40540.3; AT2G40540.3; AT2G40540.
DR Gramene; AT2G40540.4; AT2G40540.4; AT2G40540.
DR Gramene; AT2G40540.5; AT2G40540.5; AT2G40540.
DR KEGG; ath:AT2G40540; -.
DR Araport; AT2G40540; -.
DR TAIR; locus:2061838; AT2G40540.
DR eggNOG; ENOG502QPSA; Eukaryota.
DR HOGENOM; CLU_008142_2_0_1; -.
DR InParanoid; O22881; -.
DR OMA; SKKESWR; -.
DR OrthoDB; 332035at2759; -.
DR PhylomeDB; O22881; -.
DR PRO; PR:O22881; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22881; baseline and differential.
DR Genevisible; O22881; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IBA:GO_Central.
DR InterPro; IPR003855; K+_transporter.
DR PANTHER; PTHR30540; PTHR30540; 1.
DR Pfam; PF02705; K_trans; 1.
DR TIGRFAMs; TIGR00794; kup; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Potassium; Potassium transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..794
FT /note="Potassium transporter 2"
FT /id="PRO_0000209078"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..64
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..193
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..217
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 218..238
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 239..265
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..294
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 316..339
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..391
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 392..412
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 413..417
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 460..476
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 477..497
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 498..794
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MUTAGEN 167
FT /note="D->N: In kup2-5."
FT /evidence="ECO:0000269|PubMed:11826303"
FT MUTAGEN 395
FT /note="P->L: In kup2-4."
FT /evidence="ECO:0000269|PubMed:11826303"
FT MUTAGEN 419
FT /note="G->R: In kup2-1; also named shy3-1 induces a short
FT hypocotyl phenotype and decrease in cell expansion in shoot
FT tissues."
FT /evidence="ECO:0000269|PubMed:11826303"
FT MUTAGEN 560
FT /note="L->F: In kup2-6."
FT /evidence="ECO:0000269|PubMed:11826303"
SQ SEQUENCE 794 AA; 88630 MW; 4350293C21EFFAE2 CRC64;
MDLNLGKCCG SRSSKKESWR SVLLLAYQSL GVVYGDLSIS PLYVFKSTFA EDIQHSETNE
EIYGVMSFVF WTLTLVPLLK YVFIVLRADD NGEGGTFALY SLICRHVKVS LLPNRQVSDE
ALSTYKLEHP PEKNHDSCVK RYLEKHKWLH TALLLLVLLG TCMVIGDGLL TPAISVFSAV
SGLELNMSKE HHQYAVIPIT CFILVCLFSL QHFGTHRVGF VFAPIVLTWL LCISGIGLYN
IIQWNPHIYK ALSPTYMFMF LRKTRVSGWM SLGGILLCIT GAEAMFADLG HFNYAAIQIA
FTFLVYPALI LAYMGQAAYL SRHHHSAHAI GFYVSVPKCL HWPVLAVAIL ASVVGSQAII
SGTFSIINQS QSLGCFPRVK VIHTSDKMHG QIYIPEINWM LMILCIAVTI GFRDVKHLGN
ASGLAVMAVM LVTTCLTSLV IVLCWHKPPI LALAFLLFFG SIELLYFSAS LTKFREGAWL
PILLSLIFMI IMFVWHYTTI KKYEFDLQNK VSLEWLLALG PSLGISRVPG IGLVFTDLTS
GIPANFSRFV TNLPAFHRVL VFVCVKSVPV PFVPPAERYL VGRVGPVDHR SYRCIVRYGY
RDVHQDVDSF ETELVSKLAD FIRYDWHKRT QQEDDNARSV QSNESSSESR LAVIGTVAYE
IEDNLQPESV SIGFSTVESM EDVIQMAEPA PTATIRRVRF AVEENSYEDE GSTSSAEADA
ELRSELRDLL AAQEAGTAFI LGHSHVKAKQ GSSVMKRLAV NFGYNFLRRN CRGPDVALKV
PPVSLLEVGM VYVV
