POT4_ARATH
ID POT4_ARATH Reviewed; 789 AA.
AC Q9LD18; O22400; Q9SGA3;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 21-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Potassium transporter 4;
DE Short=AtKT4;
DE Short=AtKUP3;
DE Short=AtPOT4;
GN Name=POT4; Synonyms=KT4, KUP3; OrderedLocusNames=At3g02050;
GN ORFNames=F1C9.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=9477571; DOI=10.2307/3870628;
RA Kim E.J., Kwak J.M., Uozumi N., Schroeder J.I.;
RT "AtKUP1: an Arabidopsis gene encoding high-affinity potassium transport
RT activity.";
RL Plant Cell 10:51-62(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-273.
RC STRAIN=cv. Columbia;
RX PubMed=9350997; DOI=10.1016/s0014-5793(97)01125-3;
RA Quintero F.J., Blatt M.R.;
RT "A new family of K+ transporters from Arabidopsis that are conserved across
RT phyla.";
RL FEBS Lett. 415:206-211(1997).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11500563; DOI=10.1104/pp.126.4.1646;
RA Maeser P., Thomine S., Schroeder J.I., Ward J.M., Hirschi K., Sze H.,
RA Talke I.N., Amtmann A., Maathuis F.J.M., Sanders D., Harper J.F.,
RA Tchieu J., Gribskov M., Persans M.W., Salt D.E., Kim S.A., Guerinot M.L.;
RT "Phylogenetic relationships within cation transporter families of
RT Arabidopsis.";
RL Plant Physiol. 126:1646-1667(2001).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: High-affinity potassium transporter.
CC {ECO:0000269|PubMed:9477571}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Detected at very low levels in roots, stems, leaves
CC and flowers of mature plants and strongly expressed in the roots of
CC potassium-starved plants. {ECO:0000269|PubMed:9477571}.
CC -!- INDUCTION: By potassium starvation. {ECO:0000269|PubMed:9477571}.
CC -!- SIMILARITY: Belongs to the HAK/KUP transporter (TC 2.A.72.3) family.
CC {ECO:0000305}.
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DR EMBL; AF207621; AAF19432.2; -; mRNA.
DR EMBL; AC011664; AAF14830.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE73753.1; -; Genomic_DNA.
DR EMBL; AY062747; AAL32825.1; -; mRNA.
DR EMBL; BT003375; AAO30038.1; -; mRNA.
DR EMBL; AF012659; AAC49847.1; -; mRNA.
DR RefSeq; NP_186854.1; NM_111071.3.
DR AlphaFoldDB; Q9LD18; -.
DR STRING; 3702.AT3G02050.1; -.
DR iPTMnet; Q9LD18; -.
DR PaxDb; Q9LD18; -.
DR PRIDE; Q9LD18; -.
DR ProteomicsDB; 250606; -.
DR EnsemblPlants; AT3G02050.1; AT3G02050.1; AT3G02050.
DR GeneID; 821063; -.
DR Gramene; AT3G02050.1; AT3G02050.1; AT3G02050.
DR KEGG; ath:AT3G02050; -.
DR Araport; AT3G02050; -.
DR TAIR; locus:2078688; AT3G02050.
DR eggNOG; ENOG502QPSA; Eukaryota.
DR HOGENOM; CLU_008142_2_0_1; -.
DR InParanoid; Q9LD18; -.
DR OMA; AIRVSWL; -.
DR OrthoDB; 332035at2759; -.
DR PhylomeDB; Q9LD18; -.
DR PRO; PR:Q9LD18; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LD18; baseline and differential.
DR Genevisible; Q9LD18; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015079; F:potassium ion transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009932; P:cell tip growth; IBA:GO_Central.
DR GO; GO:0006813; P:potassium ion transport; IBA:GO_Central.
DR InterPro; IPR003855; K+_transporter.
DR PANTHER; PTHR30540; PTHR30540; 1.
DR Pfam; PF02705; K_trans; 1.
DR TIGRFAMs; TIGR00794; kup; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion transport; Membrane; Phosphoprotein; Potassium;
KW Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..789
FT /note="Potassium transporter 4"
FT /id="PRO_0000209080"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..68
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..195
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..270
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 271..291
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..300
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 301..321
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 322..340
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 341..361
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..392
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..424
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 425..445
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 446..450
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..478
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 500..789
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT CONFLICT 573..574
FT /note="HV -> SLF (in Ref. 1; AAF19432)"
FT /evidence="ECO:0000305"
FT CONFLICT 723..726
FT /note="DLIR -> GSDT (in Ref. 1; AAF19432)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 789 AA; 87411 MW; 07A0E89CA81B8B31 CRC64;
MAPAESGVSP RRNPSQLSWM NLSSNLILAY QSFGVVYGDL STSPLYVFPS TFIGKLHKHH
NEDAVFGAFS LIFWTLTLIP LLKYLLVLLS ADDNGEGGTF ALYSLLCRHA KLSLLPNQQA
ADEELSAYKF GPSTDTVTSS PFRTFLEKHK RLRTALLLVV LFGAAMVIGD GVLTPALSVL
SSLSGLQATE KNVTDGELLV LACVILVGLF ALQHCGTHRV AFMFAPIVII WLISIFFIGL
YNIIRWNPKI IHAVSPLYII KFFRVTGQDG WISLGGVLLS VTGTEAMFAN LGHFTSVSIR
VAFAVVVYPC LVVQYMGQAA FLSKNLGSIP NSFYDSVPDP VFWPVFVIAT LAAIVGSQAV
ITTTFSIIKQ CHALGCFPRI KVVHTSKHIY GQIYIPEINW ILMILTLAMA IGFRDTTLIG
NAYGIACMVV MFITTFFMAL VIVVVWQKSC FLAALFLGTL WIIEGVYLSA ALMKVTEGGW
VPFVLTFIFM IAMYVWHYGT RRKYSFDLHN KVSLKWLLGL GPSLGIVRVP GIGLVYSELA
TGVPAIFSHF VTNLPAFHKV VVFVCVKSVP VPHVSPEERF LIGRVCPKPY RMYRCIVRYG
YKDIQREDGD FENQLVQSIA EFIQMEASDL QSSASESQSN DGRMAVLSSQ KSLSNSILTV
SEVEEIDYAD PTIQSSKSMT LQSLRSVYED EYPQGQVRRR HVRFQLTASS GGMGSSVREE
LMDLIRAKEA GVAYIMGHSY VKSRKSSSWL KKMAIDIGYS FLRKNCRGPA VALNIPHISL
IEVGMIYYV
