POTA1_PSEAE
ID POTA1_PSEAE Reviewed; 370 AA.
AC Q9I6T2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Spermidine/putrescine import ATP-binding protein PotA 1 {ECO:0000255|HAMAP-Rule:MF_01726};
DE EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN Name=potA1 {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=PA0206;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC spermidine/putrescine import. Responsible for energy coupling to the
CC transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-Rule:MF_01726};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01726}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01726}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC {ECO:0000255|HAMAP-Rule:MF_01726}.
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DR EMBL; AE004091; AAG03595.1; -; Genomic_DNA.
DR PIR; A83621; A83621.
DR RefSeq; NP_248897.1; NC_002516.2.
DR RefSeq; WP_003106181.1; NZ_QZGE01000024.1.
DR AlphaFoldDB; Q9I6T2; -.
DR SMR; Q9I6T2; -.
DR STRING; 287.DR97_3163; -.
DR PaxDb; Q9I6T2; -.
DR PRIDE; Q9I6T2; -.
DR DNASU; 879760; -.
DR EnsemblBacteria; AAG03595; AAG03595; PA0206.
DR GeneID; 879760; -.
DR KEGG; pae:PA0206; -.
DR PATRIC; fig|208964.12.peg.214; -.
DR PseudoCAP; PA0206; -.
DR HOGENOM; CLU_000604_1_1_6; -.
DR InParanoid; Q9I6T2; -.
DR OMA; FRSESGW; -.
DR PhylomeDB; Q9I6T2; -.
DR BioCyc; PAER208964:G1FZ6-208-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0015417; F:ABC-type polyamine transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005893; Sp_pt_ABC_ATP-bd.
DR InterPro; IPR013611; Transp-assoc_OB_typ2.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF08402; TOBE_2; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF50331; SSF50331; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01187; potA; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51305; POTA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Translocase; Transport.
FT CHAIN 1..370
FT /note="Spermidine/putrescine import ATP-binding protein
FT PotA 1"
FT /id="PRO_0000286272"
FT DOMAIN 12..250
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT BINDING 48..55
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ SEQUENCE 370 AA; 40362 MW; B496D7970E64B9E7 CRC64;
MSALHSLRTH AVSIRAVRKV YGDPESGPVA LKRVDLDIRD NEFFTLLGPS GCGKTTLLRM
IAGFEFPTEG EILLYGENIA ARPPFERPVN TVFQHYALFP HMSIAENLAF GLESRPLGRS
LGKAEIAERV REMLALVQME RFADRRPGQL SGGQQQRVAL ARALAPQPRV LLLDEPLSAL
DLKLRQAMRE ELKAIQAKTG ITFIFVTHDQ EEALTMSDRI AVLSEGQVQQ VGPPEEIYER
PGNRFVADFI GETNFIEAEL LQRDGPLALY RGPGGEAFEA AARDGLAAGA RVCLSIRPER
LDLLAANTGG GLPCTVESQV YLGTDLQYRV RLADGSLLTV RTPNGTARRF ATGEAACLAV
AKDSASVLLD
