POTA2_PSEAE
ID POTA2_PSEAE Reviewed; 363 AA.
AC Q9HY19;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Spermidine/putrescine import ATP-binding protein PotA 2 {ECO:0000255|HAMAP-Rule:MF_01726};
DE EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN Name=potA2 {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=PA3607;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC spermidine/putrescine import. Responsible for energy coupling to the
CC transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-Rule:MF_01726};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01726}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01726}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC {ECO:0000255|HAMAP-Rule:MF_01726}.
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DR EMBL; AE004091; AAG06995.1; -; Genomic_DNA.
DR PIR; D83194; D83194.
DR RefSeq; NP_252297.1; NC_002516.2.
DR RefSeq; WP_003119359.1; NZ_QZGE01000001.1.
DR AlphaFoldDB; Q9HY19; -.
DR SMR; Q9HY19; -.
DR STRING; 287.DR97_4332; -.
DR PaxDb; Q9HY19; -.
DR PRIDE; Q9HY19; -.
DR DNASU; 880429; -.
DR EnsemblBacteria; AAG06995; AAG06995; PA3607.
DR GeneID; 880429; -.
DR KEGG; pae:PA3607; -.
DR PATRIC; fig|208964.12.peg.3774; -.
DR PseudoCAP; PA3607; -.
DR HOGENOM; CLU_000604_1_1_6; -.
DR InParanoid; Q9HY19; -.
DR OMA; PFSIRPE; -.
DR PhylomeDB; Q9HY19; -.
DR BioCyc; PAER208964:G1FZ6-3676-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0015417; F:ABC-type polyamine transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005893; Sp_pt_ABC_ATP-bd.
DR InterPro; IPR013611; Transp-assoc_OB_typ2.
DR Pfam; PF00005; ABC_tran; 1.
DR Pfam; PF08402; TOBE_2; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF50331; SSF50331; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01187; potA; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51305; POTA; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Reference proteome; Translocase; Transport.
FT CHAIN 1..363
FT /note="Spermidine/putrescine import ATP-binding protein
FT PotA 2"
FT /id="PRO_0000286273"
FT DOMAIN 6..236
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT BINDING 38..45
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ SEQUENCE 363 AA; 40045 MW; E22D673E91E6B9BB CRC64;
MTPPLLEIRN VTRRFGDFTA VDNVSLTINT GEFFTLLGPS GCGKTTLLRM LAGFDQPDSG
EIRLNGQDLA GVEPEKRPVH TVFQSYALFP HMSVAQNIAF PLKMAGVAKS EIDARVEQAL
KDVRLADKGG RMPTQLSGGQ RQRVAIARAL VNRPRLLLLD EPLSALDAKL REEMQIELIN
LQKDVGITFV YVTHDQGEAL ALSHRIAVMN QGRVEQLDAP ETIYSFPRSR FVADFIGQCN
LLDATVEAVD GERVRIDLRG LGEVQALKSF DAQPGEACVL TLRPEKIRLA QSVTADSDEV
HFRGRVAELL YLGDVTLYIV ELENGERLET LLPNATPGRA KFFEVGDAVE AAWRFDAGHL
VRA
