ID   POTA_MALP2              Reviewed;         594 AA.
AC   Q8EUR3;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 127.
DE   RecName: Full=Spermidine/putrescine import ATP-binding protein PotA {ECO:0000255|HAMAP-Rule:MF_01726};
DE            EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN   Name=potA {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=MYPE8570;
OS   Malacoplasma penetrans (strain HF-2) (Mycoplasma penetrans).
OC   Bacteria; Tenericutes; Mycoplasmoidales; Mycoplasmoidaceae; Malacoplasma.
OX   NCBI_TaxID=272633;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HF-2;
RX   PubMed=12466555; DOI=10.1093/nar/gkf667;
RA   Sasaki Y., Ishikawa J., Yamashita A., Oshima K., Kenri T., Furuya K.,
RA   Yoshino C., Horino A., Shiba T., Sasaki T., Hattori M.;
RT   "The complete genomic sequence of Mycoplasma penetrans, an intracellular
RT   bacterial pathogen in humans.";
RL   Nucleic Acids Res. 30:5293-5300(2002).
CC   -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC       spermidine/putrescine import. Responsible for energy coupling to the
CC       transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC         + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC         EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-Rule:MF_01726};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC       two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC       (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01726};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC       {ECO:0000255|HAMAP-Rule:MF_01726}.
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DR   EMBL; BA000026; BAC44649.1; -; Genomic_DNA.
DR   RefSeq; WP_011077678.1; NC_004432.1.
DR   AlphaFoldDB; Q8EUR3; -.
DR   STRING; 272633.26454320; -.
DR   EnsemblBacteria; BAC44649; BAC44649; BAC44649.
DR   KEGG; mpe:MYPE8570; -.
DR   eggNOG; COG3842; Bacteria.
DR   HOGENOM; CLU_000604_1_1_14; -.
DR   OMA; IRQKMQV; -.
DR   OrthoDB; 1200451at2; -.
DR   Proteomes; UP000002522; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015417; F:ABC-type polyamine transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51305; POTA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Translocase; Transport.
FT   CHAIN           1..594
FT                   /note="Spermidine/putrescine import ATP-binding protein
FT                   PotA"
FT                   /id="PRO_0000286261"
FT   DOMAIN          24..435
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT   REGION          125..304
FT                   /note="Insert"
FT   BINDING         57..64
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ   SEQUENCE   594 AA;  69136 MW;  374824F3501AF6F4 CRC64;
     MKTKDNEVIN SESEFSEKDV PNFIEIKKIN KTYPDGYVAV KNINFEIKKG EFVTILGPSG
     CGKTTILKMI GGFELPTSGK ILVNKIDIKD LPIQRRPTAT VFQDYALFPN MNVEKNIAYG
     LTEIRKPIEN VSADYQKESE KYFNDCLKKS KSKIKDIERK RDGFLKDIQK LENKINNSKI
     LSEVNTMTEE EYEEKIETLE KEYFEKNKKE LHKSIPVKVK FIEFINNTLS FFRINKNIDF
     KANETDELVQ TYLKYEKAYR VNLITKQEID YLNHKAADLD YWVSYWQNYP YQEKEWFDKK
     KLTRKLTKQE IKEEVQQIIK IIGLEGKEKK WPSDLSGGMQ QRVALARALV IKPETLLLDE
     PLSALDAKVR AQMQQELKNL HKKFGITFIL VTHDQEEALT LSDKIIVMSQ GKIQQIGTPN
     EIYDLPANNW VANFIGKANI LNATYLKGNK IKLFDNVLNA DSRYKDKFKE NEEVNVMIRP
     EDFDVVGKDK GKIKVTVLET TYKGLMWELI CEFEGVLLTL EAVNKVNLEQ EIYLTWDDED
     MHIMKKDDEN DTYTDESSEF LALTKNAFKK KIKEIKSKKN KNKVNGKKGD KNDN