ID   POTA_MYCGA              Reviewed;         501 AA.
AC   Q7NB11;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 2.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Spermidine/putrescine import ATP-binding protein PotA {ECO:0000255|HAMAP-Rule:MF_01726};
DE            EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN   Name=potA {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=MYCGA4690;
GN   ORFNames=MGA_0135;
OS   Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
OS   (Mycoplasmoides gallisepticum).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=710127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=R(low / passage 15 / clone 2);
RX   PubMed=12949158; DOI=10.1099/mic.0.26427-0;
RA   Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F.,
RA   Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.;
RT   "The complete genome sequence of the avian pathogen Mycoplasma
RT   gallisepticum strain R(low).";
RL   Microbiology 149:2307-2316(2003).
CC   -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC       spermidine/putrescine import. Responsible for energy coupling to the
CC       transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC         + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC         EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-Rule:MF_01726};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC       two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC       (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01726};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC       {ECO:0000255|HAMAP-Rule:MF_01726}.
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DR   EMBL; AE015450; AAP56819.2; -; Genomic_DNA.
DR   RefSeq; WP_011113718.1; NC_004829.2.
DR   AlphaFoldDB; Q7NB11; -.
DR   KEGG; mga:MGA_0135; -.
DR   PATRIC; fig|233150.7.peg.527; -.
DR   HOGENOM; CLU_000604_1_1_14; -.
DR   OrthoDB; 1200451at2; -.
DR   Proteomes; UP000001418; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015417; F:ABC-type polyamine transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51305; POTA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Translocase; Transport.
FT   CHAIN           1..501
FT                   /note="Spermidine/putrescine import ATP-binding protein
FT                   PotA"
FT                   /id="PRO_0000286255"
FT   DOMAIN          7..385
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT   REGION          108..254
FT                   /note="Insert"
FT   BINDING         40..47
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ   SEQUENCE   501 AA;  57850 MW;  CF2398EF363E84D6 CRC64;
     MEDKVFIQLR NVNKTFDDGF VAVRNLNLDI NKSEFVTLLG PSGCGKTTTL KLLAGFEQPT
     YGQIKINGID IKDMPVHKRP FATVFQDYAL FPNMTVYNNV CYGLKIMRVP KENTDPKLAV
     EAENIKKEAE KKAAAKIKDL SRKKNDLSNK LDKIRKEYEK NDWMLENDEM RFAQYQEELI
     KLEAQLSEAY EQSEINKLNQ EIVELKTNYQ KKIPIDNRYD KVLKELENAD GWTSYWETYP
     IMKKEHFENK VLTRRLTKKE IEERANNMIK LVGLTGKEDK YPSELSGGMQ QRVALARALV
     IEPETLLLDE PLSALDAKVR KQLQNELKRI HKELNITFIL VTHDQEEALS LSDKIVVMSQ
     GDIEQVGKPN AIYDSPINEW TAKFIGAANI FDGKYLGDYK IELNSGDIID TDEEYEFKKG
     DNVRVLIRPE DFDVVEKDQG FFNVEVIKTS YKGVLWEVEC LMSDKTKIKV DNIDEVKVGA
     IVGLKFDEMD VHMMRKEEED V