ID   POTA_MYCPU              Reviewed;         458 AA.
AC   Q98QE1;
DT   01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   03-AUG-2022, entry version 131.
DE   RecName: Full=Spermidine/putrescine import ATP-binding protein PotA {ECO:0000255|HAMAP-Rule:MF_01726};
DE            EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN   Name=potA {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=MYPU_4250;
OS   Mycoplasmopsis pulmonis (strain UAB CTIP) (Mycoplasma pulmonis).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasmopsis.
OX   NCBI_TaxID=272635;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UAB CTIP;
RX   PubMed=11353084; DOI=10.1093/nar/29.10.2145;
RA   Chambaud I., Heilig R., Ferris S., Barbe V., Samson D., Galisson F.,
RA   Moszer I., Dybvig K., Wroblewski H., Viari A., Rocha E.P.C., Blanchard A.;
RT   "The complete genome sequence of the murine respiratory pathogen Mycoplasma
RT   pulmonis.";
RL   Nucleic Acids Res. 29:2145-2153(2001).
CC   -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC       spermidine/putrescine import. Responsible for energy coupling to the
CC       transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC         + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC         EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-Rule:MF_01726};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC       two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC       (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01726};
CC       Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC       {ECO:0000255|HAMAP-Rule:MF_01726}.
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DR   EMBL; AL445564; CAC13598.1; -; Genomic_DNA.
DR   PIR; A90565; A90565.
DR   RefSeq; WP_010925226.1; NC_002771.1.
DR   AlphaFoldDB; Q98QE1; -.
DR   SMR; Q98QE1; -.
DR   STRING; 272635.MYPU_4250; -.
DR   EnsemblBacteria; CAC13598; CAC13598; CAC13598.
DR   KEGG; mpu:MYPU_4250; -.
DR   eggNOG; COG3839; Bacteria.
DR   HOGENOM; CLU_000604_1_1_14; -.
DR   OMA; IRQKMQV; -.
DR   OrthoDB; 1200451at2; -.
DR   BioCyc; MPUL272635:G1GT6-430-MON; -.
DR   Proteomes; UP000000528; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015417; F:ABC-type polyamine transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR013611; Transp-assoc_OB_typ2.
DR   Pfam; PF00005; ABC_tran; 2.
DR   Pfam; PF08402; TOBE_2; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51305; POTA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Reference proteome; Translocase; Transport.
FT   CHAIN           1..458
FT                   /note="Spermidine/putrescine import ATP-binding protein
FT                   PotA"
FT                   /id="PRO_0000286262"
FT   DOMAIN          20..341
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT   REGION          120..210
FT                   /note="Insert"
FT   BINDING         52..59
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ   SEQUENCE   458 AA;  53108 MW;  A307637903860F12 CRC64;
     MNNINTTLIE GRKSHSKFHI SLKNVDKNYG DKKILDDVNL SIKKGEFVTL LGPSGSGKTT
     ILRLIGGFEW TTRGEILFDG LDIKDLSPHK RETSTIFQDY ALFQHLSVTG NIKYGLKLKR
     YPKDPSSIDP GIYKNLEKKK KEWEKFAKSK MKQLDKTQEE YEKILKSKNL SRGKRQKYQS
     WLDDSDFKYS YWETYVVNKV EQFEKHHLTR KITKDEINEE ITKMIKLVGL EGSENKKISE
     LSGGMKQRVA LARSLVIEPE ILLLDEPLSA LDAKIRTKMQ RLLIELQKKI GITFIFVTHD
     QDEALELSDR IAVIRDGKIE QFDTPKQIYD YPINKWVANF IGEANFFDVR FVEKNKALLL
     GKHIPTIHTE FEEGQKLDGL IRPEDIDISL EKGHFEGVVE KIIYKGSYYF ISVNVEGKII
     EVETNDYFEV GKKVWITWDL DALHLMAKDH KGFMANDF