ID   POTA_SALTY              Reviewed;         378 AA.
AC   P40790;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   13-DEC-2001, sequence version 2.
DT   03-AUG-2022, entry version 138.
DE   RecName: Full=Spermidine/putrescine import ATP-binding protein PotA {ECO:0000255|HAMAP-Rule:MF_01726};
DE            EC=7.6.2.11 {ECO:0000255|HAMAP-Rule:MF_01726};
GN   Name=potA {ECO:0000255|HAMAP-Rule:MF_01726}; OrderedLocusNames=STM1226;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-63.
RX   PubMed=1904438; DOI=10.1128/jb.173.11.3554-3558.1991;
RA   Miller C.G., Miller J.L., Bagga D.A.;
RT   "Cloning and nucleotide sequence of the anaerobically regulated pepT gene
RT   of Salmonella typhimurium.";
RL   J. Bacteriol. 173:3554-3558(1991).
CC   -!- FUNCTION: Part of the ABC transporter complex PotABCD involved in
CC       spermidine/putrescine import. Responsible for energy coupling to the
CC       transport system. {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + polyamine-[polyamine-binding protein]Side 1 = ADP
CC         + phosphate + polyamineSide 2 + [polyamine-binding protein]Side 1.;
CC         EC=7.6.2.11; Evidence={ECO:0000255|HAMAP-Rule:MF_01726};
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotA),
CC       two transmembrane proteins (PotB and PotC) and a solute-binding protein
CC       (PotD). {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01726}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01726}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily.
CC       Spermidine/putrescine importer (TC 3.A.1.11.1) family.
CC       {ECO:0000255|HAMAP-Rule:MF_01726}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=M62725; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AE006468; AAL20155.1; -; Genomic_DNA.
DR   EMBL; M62725; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_460196.1; NC_003197.2.
DR   RefSeq; WP_000531607.1; NC_003197.2.
DR   AlphaFoldDB; P40790; -.
DR   SMR; P40790; -.
DR   STRING; 99287.STM1226; -.
DR   PaxDb; P40790; -.
DR   PRIDE; P40790; -.
DR   EnsemblBacteria; AAL20155; AAL20155; STM1226.
DR   GeneID; 1252744; -.
DR   KEGG; stm:STM1226; -.
DR   PATRIC; fig|99287.12.peg.1297; -.
DR   HOGENOM; CLU_000604_1_1_6; -.
DR   OMA; IHVMRFN; -.
DR   PhylomeDB; P40790; -.
DR   BioCyc; SENT99287:STM1226-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0015594; F:ABC-type putrescine transporter activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   CDD; cd03300; ABC_PotA_N; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR008995; Mo/tungstate-bd_C_term_dom.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR017879; PotA_ATP-bd.
DR   InterPro; IPR005893; Sp_pt_ABC_ATP-bd.
DR   InterPro; IPR013611; Transp-assoc_OB_typ2.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF08402; TOBE_2; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF50331; SSF50331; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR01187; potA; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51305; POTA; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Translocase; Transport.
FT   CHAIN           1..378
FT                   /note="Spermidine/putrescine import ATP-binding protein
FT                   PotA"
FT                   /id="PRO_0000092752"
FT   DOMAIN          18..248
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
FT   BINDING         50..57
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01726"
SQ   SEQUENCE   378 AA;  42840 MW;  470FEC6B00F17216 CRC64;
     MGQSKKLNNQ PRSLSPLVLL SGISKSFDGK EVISQLDLTI NNGEFLTLLG PSGCGKTTVL
     RLIAGLETVD AGHIMLDNQD ITHVPAENRY VNTVFQSYAL FPHMTVFENV AFGLRMQKTP
     AAEIAPRVTD ALRMVQLEEF AQRKPHQLSG GQQQRVAIAR AVVNKPRLLL LDESLSALDY
     KLRKQMQNEL KALQRKLGIT FVFVTHDQEE ALTMSDRIVV MRNGVIEQDG TPREIYEEPK
     NLFVAGFIGE INRFDATVIE RLDEQRVRAS VEGRECNIYV NFAVEPGQKL NVLLRPEDLR
     VEEINDDNHI EGLIGYVRER NYKGMTLESV VELENGKMVM VSEFFNEDDP DFDHSLDQKM
     AISWVESWEV VLADEEHK