POTC_ECOLI
ID POTC_ECOLI Reviewed; 264 AA.
AC P0AFK6; P23859;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Spermidine/putrescine transport system permease protein PotC;
GN Name=potC; OrderedLocusNames=b1124, JW1110;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=1939142; DOI=10.1016/s0021-9258(18)54799-2;
RA Furuchi T., Kashiwagi K., Kobayashi H., Igarashi K.;
RT "Characteristics of the gene for a spermidine and putrescine transport
RT system that maps at 15 min on the Escherichia coli chromosome.";
RL J. Biol. Chem. 266:20928-20933(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Required for the activity of the bacterial periplasmic
CC transport system of putrescine and spermidine.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:15919996}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; M64519; AAC37040.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74208.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35944.1; -; Genomic_DNA.
DR PIR; C40840; C40840.
DR RefSeq; NP_415642.1; NC_000913.3.
DR RefSeq; WP_000580316.1; NZ_SSUV01000046.1.
DR AlphaFoldDB; P0AFK6; -.
DR SMR; P0AFK6; -.
DR BioGRID; 4260092; 49.
DR ComplexPortal; CPX-4383; Spermidine ABC transporter complex.
DR DIP; DIP-48152N; -.
DR IntAct; P0AFK6; 1.
DR STRING; 511145.b1124; -.
DR TCDB; 3.A.1.11.1; the atp-binding cassette (abc) superfamily.
DR jPOST; P0AFK6; -.
DR PaxDb; P0AFK6; -.
DR PRIDE; P0AFK6; -.
DR EnsemblBacteria; AAC74208; AAC74208; b1124.
DR EnsemblBacteria; BAA35944; BAA35944; BAA35944.
DR GeneID; 67414317; -.
DR GeneID; 945691; -.
DR KEGG; ecj:JW1110; -.
DR KEGG; eco:b1124; -.
DR PATRIC; fig|1411691.4.peg.1143; -.
DR EchoBASE; EB0744; -.
DR eggNOG; COG1177; Bacteria.
DR HOGENOM; CLU_016047_3_0_6; -.
DR InParanoid; P0AFK6; -.
DR OMA; NKFGMKW; -.
DR PhylomeDB; P0AFK6; -.
DR BioCyc; EcoCyc:POTC-MON; -.
DR BioCyc; MetaCyc:POTC-MON; -.
DR PRO; PR:P0AFK6; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; TAS:EcoCyc.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015417; F:ABC-type polyamine transporter activity; IDA:EcoCyc.
DR GO; GO:0015847; P:putrescine transport; IDA:EcoCyc.
DR GO; GO:1903711; P:spermidine transmembrane transport; IDA:EcoCyc.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..264
FT /note="Spermidine/putrescine transport system permease
FT protein PotC"
FT /id="PRO_0000060181"
FT TOPO_DOM 1..7
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 8..27
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 28..65
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..85
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 86..100
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..120
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 121..128
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 129..148
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 149..176
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..196
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 197..231
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..251
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 252..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 60..248
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 264 AA; 29111 MW; D21619C91184BB59 CRC64;
MIGRLLRGGF MTAIYAYLYI PIIILIVNSF NSSRFGINWQ GFTTKWYSLL MNNDSLLQAA
QHSLTMAVFS ATFATLIGSL TAVALYRYRF RGKPFVSGML FVVMMSPDIV MAISLLVLFM
LLGIQLGFWS LLFSHITFCL PFVVVTVYSR LKGFDVRMLE AAKDLGASEF TILRKIILPL
AMPAVAAGWV LSFTLSMDDV VVSSFVTGPS YEILPLKIYS MVKVGVSPEV NALATILLVL
SLVMVIASQL IARDKTKGNT GDVK
