POTE_ECOL6
ID POTE_ECOL6 Reviewed; 439 AA.
AC P0AAF2; P24170;
DT 11-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Putrescine transporter PotE {ECO:0000255|HAMAP-Rule:MF_02073};
DE AltName: Full=Putrescine-proton symporter / putrescine-ornithine antiporter {ECO:0000255|HAMAP-Rule:MF_02073};
GN Name=potE {ECO:0000255|HAMAP-Rule:MF_02073}; OrderedLocusNames=c0776;
OS Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=199310;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CFT073 / ATCC 700928 / UPEC;
RX PubMed=12471157; DOI=10.1073/pnas.252529799;
RA Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA Donnenberg M.S., Blattner F.R.;
RT "Extensive mosaic structure revealed by the complete genome sequence of
RT uropathogenic Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC -!- FUNCTION: Catalyzes both the uptake and excretion of putrescine. The
CC uptake of putrescine is dependent on the membrane potential and the
CC excretion involves putrescine-ornithine antiporter activity.
CC {ECO:0000255|HAMAP-Rule:MF_02073}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + putrescine(in) = H(+)(out) + putrescine(out);
CC Xref=Rhea:RHEA:28891, ChEBI:CHEBI:15378, ChEBI:CHEBI:326268;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_02073};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:28893;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_02073};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-ornithine(out) + putrescine(in) = L-ornithine(in) +
CC putrescine(out); Xref=Rhea:RHEA:28827, ChEBI:CHEBI:46911,
CC ChEBI:CHEBI:326268; Evidence={ECO:0000255|HAMAP-Rule:MF_02073};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28828;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_02073};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02073}; Multi-pass membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_02073}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Basic amino acid/polyamine antiporter (APA) (TC 2.A.3.2)
CC family. {ECO:0000255|HAMAP-Rule:MF_02073}.
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DR EMBL; AE014075; AAN79249.1; -; Genomic_DNA.
DR RefSeq; WP_000075845.1; NC_004431.1.
DR AlphaFoldDB; P0AAF2; -.
DR SMR; P0AAF2; -.
DR STRING; 199310.c0776; -.
DR EnsemblBacteria; AAN79249; AAN79249; c0776.
DR GeneID; 66671040; -.
DR KEGG; ecc:c0776; -.
DR eggNOG; COG0531; Bacteria.
DR HOGENOM; CLU_007946_1_0_6; -.
DR OMA; AEKVMSV; -.
DR BioCyc; ECOL199310:C0776-MON; -.
DR Proteomes; UP000001410; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0015496; F:putrescine:ornithine antiporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR HAMAP; MF_02073; Putrescine_transp; 1.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR004754; Amino_acid_antiprt.
DR InterPro; IPR027566; Symport/antiport_PotE.
DR Pfam; PF13520; AA_permease_2; 1.
DR TIGRFAMs; TIGR00905; 2A0302; 1.
DR TIGRFAMs; TIGR04299; antiport_PotE; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Antiport; Cell inner membrane; Cell membrane;
KW Membrane; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..439
FT /note="Putrescine transporter PotE"
FT /id="PRO_0000054251"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 40..60
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 321..341
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 354..374
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 387..407
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
FT TRANSMEM 410..430
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02073"
SQ SEQUENCE 439 AA; 46495 MW; C6F800284DA8C5C8 CRC64;
MSQAKSNKMG VVQLTILTMV NMMGSGIIML PTKLAEVGTI SIISWLVTAV GSMALAWAFA
KCGMFSRKSG GMGGYAEYAF GKSGNFMANY TYGVSLLIAN VAIAISAVGY GTELLGASLS
PVQIGLATIG VLWICTVANF GGARITGQIS SITVWGVIIP VVGLCIIGWF WFSPTLYVDS
WNPHHAPFFS AVGSSIAMTL WAFLGLESAC ANTDVVENPE RNVPIAVLGG TLGAAVIYIV
STNVIAGIVP NMELANSTAP FGLAFAQMFT PEVGKVIMAL MVMSCCGSLL GWQFTIAQVF
KSSSDEGYFP KIFSRVTKVD APVQGMLTIV IIQSGLALMT ISPSLNSQFN VLVNLAVVTN
IIPYILSMAA LVIIQKVANV PPSKAKVANF VAFVGAMYSF YALYSSGEEA MLYGSIVTFL
GWTLYGLVSP RFELKNKHG
