POTH_ECOLI
ID POTH_ECOLI Reviewed; 317 AA.
AC P31135;
DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1993, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Putrescine transport system permease protein PotH {ECO:0000305};
GN Name=potH {ECO:0000303|PubMed:8416922}; OrderedLocusNames=b0856, JW0840;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBUNIT.
RX PubMed=8416922; DOI=10.1016/s0021-9258(18)54126-0;
RA Pistocchi R., Kashiwagi K., Miyamoto S., Nukui E., Sadakata Y.,
RA Kobayashi H., Igarashi K.;
RT "Characteristics of the operon for a putrescine transport system that maps
RT at 19 minutes on the Escherichia coli chromosome.";
RL J. Biol. Chem. 268:146-152(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP FUNCTION, ACTIVITY REGULATION, AND SUBUNIT.
RX PubMed=23719730; DOI=10.1007/s00726-013-1517-x;
RA Terui Y., Saroj S.D., Sakamoto A., Yoshida T., Higashi K., Kurihara S.,
RA Suzuki H., Toida T., Kashiwagi K., Igarashi K.;
RT "Properties of putrescine uptake by PotFGHI and PuuP and their
RT physiological significance in Escherichia coli.";
RL Amino Acids 46:661-670(2014).
CC -!- FUNCTION: Part of the ABC transporter complex PotFGHI involved in
CC putrescine uptake (PubMed:8416922, PubMed:23719730). Responsible for
CC the translocation of the substrate across the membrane (Probable).
CC Imports putrescine for maintenance of the optimal concentration of
CC polyamines necessary for cell growth in the presence of glucose
CC (PubMed:23719730). {ECO:0000269|PubMed:23719730,
CC ECO:0000269|PubMed:8416922, ECO:0000305}.
CC -!- ACTIVITY REGULATION: Transport is feedback inhibited by intracellular
CC polyamines. {ECO:0000269|PubMed:23719730}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotG),
CC two transmembrane proteins (PotH and PotI) and a solute-binding protein
CC (PotF). {ECO:0000269|PubMed:23719730, ECO:0000269|PubMed:8416922}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; M93239; AAA24411.1; -; Genomic_DNA.
DR EMBL; U00096; AAC73943.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35567.1; -; Genomic_DNA.
DR PIR; C45313; C45313.
DR RefSeq; NP_415377.1; NC_000913.3.
DR RefSeq; WP_000105430.1; NZ_SSZK01000002.1.
DR AlphaFoldDB; P31135; -.
DR SMR; P31135; -.
DR BioGRID; 4259995; 14.
DR BioGRID; 849849; 1.
DR ComplexPortal; CPX-4384; Putrescine ABC transporter complex.
DR IntAct; P31135; 1.
DR STRING; 511145.b0856; -.
DR TCDB; 3.A.1.11.2; the atp-binding cassette (abc) superfamily.
DR PaxDb; P31135; -.
DR PRIDE; P31135; -.
DR EnsemblBacteria; AAC73943; AAC73943; b0856.
DR EnsemblBacteria; BAA35567; BAA35567; BAA35567.
DR GeneID; 66670870; -.
DR GeneID; 945475; -.
DR KEGG; ecj:JW0840; -.
DR KEGG; eco:b0856; -.
DR PATRIC; fig|1411691.4.peg.1421; -.
DR EchoBASE; EB1588; -.
DR eggNOG; COG1176; Bacteria.
DR HOGENOM; CLU_016047_18_3_6; -.
DR InParanoid; P31135; -.
DR OMA; YPIAYGM; -.
DR PhylomeDB; P31135; -.
DR BioCyc; EcoCyc:POTH-MON; -.
DR BioCyc; MetaCyc:POTH-MON; -.
DR PRO; PR:P31135; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; TAS:EcoCyc.
DR GO; GO:0055052; C:ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing; IC:ComplexPortal.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015847; P:putrescine transport; IDA:EcoCyc.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..317
FT /note="Putrescine transport system permease protein PotH"
FT /id="PRO_0000060188"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:15919996"
FT TRANSMEM 32..51
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 52..104
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:15919996"
FT TRANSMEM 105..124
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 125..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:15919996"
FT TRANSMEM 134..153
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 154..184
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:15919996"
FT TRANSMEM 185..204
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 205..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:15919996"
FT TRANSMEM 240..259
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 260..284
FT /note="Periplasmic"
FT /evidence="ECO:0000305|PubMed:15919996"
FT TRANSMEM 285..304
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TOPO_DOM 305..317
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
FT DOMAIN 99..305
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ SEQUENCE 317 AA; 35490 MW; 5085C43C311F490A CRC64;
MSTLEPAAQS KPPGGFKLWL SQLQMKHGRK LVIALPYIWL ILLFLLPFLI VFKISLAEMA
RAIPPYTELM EWADGQLSIT LNLGNFLQLT DDPLYFDAYL QSLQVAAIST FCCLLIGYPL
AWAVAHSKPS TRNILLLLVI LPSWTSFLIR VYAWMGILKN NGVLNNFLLW LGVIDQPLTI
LHTNLAVYIG IVYAYVPFMV LPIYTALIRI DYSLVEAALD LGARPLKTFF TVIVPLTKGG
IIAGSMLVFI PAVGEFVIPE LLGGPDSIMI GRVLWQEFFN NRDWPVASAV AIIMLLLLIV
PIMWFHKHQQ KSVGEHG
