ID   POTI_ECOL6              Reviewed;         281 AA.
AC   P0AFL2; P31136;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Putrescine transport system permease protein PotI {ECO:0000250|UniProtKB:P0AFL1};
GN   Name=potI {ECO:0000250|UniProtKB:P0AFL1}; OrderedLocusNames=c0990;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Part of the ABC transporter complex PotFGHI involved in
CC       putrescine uptake. Responsible for the translocation of the substrate
CC       across the membrane. {ECO:0000250|UniProtKB:P0AFL1}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PotG),
CC       two transmembrane proteins (PotH and PotI) and a solute-binding protein
CC       (PotF). {ECO:0000250|UniProtKB:P0AFL1}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000250|UniProtKB:P0AFL1}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN79463.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE014075; AAN79463.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_001061658.1; NC_004431.1.
DR   AlphaFoldDB; P0AFL2; -.
DR   SMR; P0AFL2; -.
DR   STRING; 199310.c0990; -.
DR   EnsemblBacteria; AAN79463; AAN79463; c0990.
DR   GeneID; 67413899; -.
DR   KEGG; ecc:c0990; -.
DR   eggNOG; COG1177; Bacteria.
DR   HOGENOM; CLU_016047_3_0_6; -.
DR   OMA; FPMFVWG; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..281
FT                   /note="Putrescine transport system permease protein PotI"
FT                   /id="PRO_0000060190"
FT   TOPO_DOM        1..13
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        14..33
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        34..68
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        89..115
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        116..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        136..140
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        161..186
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        187..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        207..243
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        244..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TOPO_DOM        264..281
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          65..260
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   281 AA;  30541 MW;  A1B557CE2FC7C9EB CRC64;
     MNNLPVVRSP WRIVILLLGF TFLYAPMLML VIYSFNSSKL VTVWAGWSTR WYGELLRDDA
     MMSAVGLSLT IAACAATAAA ILGTIAAVVL VRFGRFRGSN GFAFMITAPL VMPDVITGLS
     LLLLFVALAH AIGWPADRGM LTIWLAHVTF CTAYVAVVIS SRLRELDRSI EEAAMDLGAT
     PLKVFFVITL PMIMPAIISG WLLAFTLSLD DLVIASFVSG PGATTLPMLV FSSVRMGVNP
     EINALATLIL GAVGIVGFIA WYLMARAEKQ RIRDIQRARR G