ID   POTM1_SOLTU             Reviewed;          47 AA.
AC   P84813;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   17-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Potamin-1;
DE            Short=PT-1;
DE   Flags: Fragment;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   STRAIN=cv. Gogu {ECO:0000269|PubMed:15809084};
RC   TISSUE=Tuber {ECO:0000269|PubMed:15809084};
RX   PubMed=15809084; DOI=10.1016/j.bbrc.2005.03.057;
RA   Kim J.-Y., Park S.-C., Kim M.-H., Lim H.-T., Park Y., Hahm K.-S.;
RT   "Antimicrobial activity studies on a trypsin-chymotrypsin protease
RT   inhibitor obtained from potato.";
RL   Biochem. Biophys. Res. Commun. 330:921-927(2005).
CC   -!- FUNCTION: Inhibitor of serine proteases chymotrypsin, papain and
CC       trypsin. Has strong antifungal activity against C.albicans and
CC       R.solani. Has antibacterial activity against the Gram-positive
CC       bacterium C.michiganense, but lacks antibacterial activity against the
CC       Gram-positive bacterium S.aureus. Lacks hemolytic activity against
CC       human erythrocytes. {ECO:0000269|PubMed:15809084}.
CC   -!- MASS SPECTROMETRY: Mass=5600; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:15809084};
CC   -!- SIMILARITY: Belongs to the protease inhibitor I20 (potato type II
CC       proteinase inhibitor) family. {ECO:0000255}.
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DR   AlphaFoldDB; P84813; -.
DR   SMR; P84813; -.
DR   PRIDE; P84813; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P84813; baseline and differential.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   InterPro; IPR003465; Prot_inh_I20.
DR   Pfam; PF02428; Prot_inhib_II; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Disulfide bond;
KW   Fungicide; Protease inhibitor; Reference proteome; Repeat;
KW   Serine protease inhibitor.
FT   CHAIN           1..>47
FT                   /note="Potamin-1"
FT                   /id="PRO_0000253944"
FT   SITE            38..39
FT                   /note="Reactive bond for chymotrypsin"
FT                   /evidence="ECO:0000250|UniProtKB:P05119"
FT   DISULFID        3..40
FT                   /evidence="ECO:0000250|UniProtKB:P05119"
FT   DISULFID        6..24
FT                   /evidence="ECO:0000250|UniProtKB:P05119"
FT   DISULFID        7..36
FT                   /evidence="ECO:0000250|UniProtKB:P05119"
FT   DISULFID        13..?
FT                   /evidence="ECO:0000250|UniProtKB:P05119"
FT   NON_TER         47
FT                   /evidence="ECO:0000303|PubMed:15809084"
SQ   SEQUENCE   47 AA;  4833 MW;  DF50D305C7285E1F CRC64;
     DICTNCCAGT KGCNTTSANG AFICEGQSDP KKPKACPLNC DPHIAYA