POXA_DICDI
ID POXA_DICDI Reviewed; 531 AA.
AC Q6TMK4; Q54ZU1;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Peroxinectin A;
DE EC=1.11.1.7;
DE Flags: Precursor;
GN Name=poxA; ORFNames=DDB_G0277275;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND INDUCTION BY
RP SRFA.
RX PubMed=15075287; DOI=10.1128/ec.3.2.564-566.2004;
RA Escalante R., Iranfar N., Sastre L., Loomis W.F.;
RT "Identification of genes dependent on the MADS box transcription factor
RT SrfA in Dictyostelium discoideum development.";
RL Eukaryot. Cell 3:564-566(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP DEVELOPMENTAL STAGE, AND INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=17950724; DOI=10.1016/j.ydbio.2007.08.055;
RA Prabhu Y., Mondal S., Eichinger L., Noegel A.A.;
RT "A GPCR involved in post aggregation events in Dictyostelium discoideum.";
RL Dev. Biol. 312:29-43(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 a phenolic donor + H2O2 = 2 a phenolic radical donor + 2
CC H2O; Xref=Rhea:RHEA:56136, ChEBI:CHEBI:15377, ChEBI:CHEBI:16240,
CC ChEBI:CHEBI:139520, ChEBI:CHEBI:139521; EC=1.11.1.7;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in late developmental stage, exclusively
CC during culmination. {ECO:0000269|PubMed:15075287,
CC ECO:0000269|PubMed:17950724}.
CC -!- INDUCTION: Induced by srfA, during development. Down-regulated in grlA
CC null-cells at 16 hours of starvation. {ECO:0000269|PubMed:15075287,
CC ECO:0000269|PubMed:17950724}.
CC -!- SIMILARITY: Belongs to the peroxidase family. {ECO:0000255|PROSITE-
CC ProRule:PRU00298}.
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DR EMBL; AY392429; AAQ98871.1; -; Genomic_DNA.
DR EMBL; AAFI02000019; EAL68824.1; -; Genomic_DNA.
DR RefSeq; XP_642775.1; XM_637683.1.
DR PDB; 6ERC; X-ray; 2.50 A; A/B=21-531.
DR PDBsum; 6ERC; -.
DR AlphaFoldDB; Q6TMK4; -.
DR SMR; Q6TMK4; -.
DR STRING; 44689.DDB0191269; -.
DR PeroxiBase; 4094; DdPxDo01.
DR PaxDb; Q6TMK4; -.
DR EnsemblProtists; EAL68824; EAL68824; DDB_G0277275.
DR GeneID; 8620966; -.
DR KEGG; ddi:DDB_G0277275; -.
DR dictyBase; DDB_G0277275; poxA.
DR eggNOG; KOG2408; Eukaryota.
DR HOGENOM; CLU_006087_2_2_1; -.
DR InParanoid; Q6TMK4; -.
DR OMA; FSLPYGW; -.
DR PhylomeDB; Q6TMK4; -.
DR Reactome; R-DDI-209968; Thyroxine biosynthesis.
DR Reactome; R-DDI-2243919; Crosslinking of collagen fibrils.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-8941413; Events associated with phagocytolytic activity of PMN cells.
DR PRO; PR:Q6TMK4; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase.
DR GO; GO:0005576; C:extracellular region; TAS:dictyBase.
DR GO; GO:0020037; F:heme binding; IDA:dictyBase.
DR GO; GO:0004447; F:iodide peroxidase activity; IDA:dictyBase.
DR GO; GO:0004601; F:peroxidase activity; IDA:dictyBase.
DR GO; GO:0036393; F:thiocyanate peroxidase activity; IDA:dictyBase.
DR GO; GO:0042742; P:defense response to bacterium; IMP:dictyBase.
DR GO; GO:0042744; P:hydrogen peroxide catabolic process; IEA:UniProtKB-KW.
DR GO; GO:0006955; P:immune response; ISS:dictyBase.
DR GO; GO:0006979; P:response to oxidative stress; IEA:InterPro.
DR Gene3D; 1.10.640.10; -; 1.
DR InterPro; IPR019791; Haem_peroxidase_animal.
DR InterPro; IPR010255; Haem_peroxidase_sf.
DR InterPro; IPR037120; Haem_peroxidase_sf_animal.
DR Pfam; PF03098; An_peroxidase; 1.
DR PRINTS; PR00457; ANPEROXIDASE.
DR SUPFAM; SSF48113; SSF48113; 1.
DR PROSITE; PS50292; PEROXIDASE_3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Glycoprotein; Hydrogen peroxide; Oxidoreductase; Peroxidase;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..531
FT /note="Peroxinectin A"
FT /id="PRO_0000386630"
FT ACT_SITE 101
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00298"
FT SITE 233
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00298"
FT CARBOHYD 62
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 131
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 338
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT STRAND 27..29
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 32..36
FT /evidence="ECO:0007829|PDB:6ERC"
FT TURN 37..40
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 68..75
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 90..100
FT /evidence="ECO:0007829|PDB:6ERC"
FT TURN 101..103
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 108..112
FT /evidence="ECO:0007829|PDB:6ERC"
FT TURN 122..124
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 137..139
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 141..144
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 148..150
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 159..164
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 173..176
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 180..186
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 197..199
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 202..204
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 232..235
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 238..261
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 267..288
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 290..295
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 314..318
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 320..323
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 324..327
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 330..335
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 341..346
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 347..349
FT /evidence="ECO:0007829|PDB:6ERC"
FT TURN 350..352
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 354..357
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 362..370
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 376..378
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 382..385
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 389..391
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 399..410
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 415..421
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 430..433
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 437..446
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 450..452
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 455..461
FT /evidence="ECO:0007829|PDB:6ERC"
FT STRAND 469..471
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 472..487
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 496..501
FT /evidence="ECO:0007829|PDB:6ERC"
FT TURN 502..504
FT /evidence="ECO:0007829|PDB:6ERC"
FT HELIX 510..517
FT /evidence="ECO:0007829|PDB:6ERC"
SQ SEQUENCE 531 AA; 60797 MW; E2444F8EAFADF9EF CRC64;
MRLNLISFFI IFTILVSISN SQEFRSYTGE GNNKQNPKQG SIFTPFIRLA NPIKFNKNGF
PNITNQPSRA ISNIIFDQQT HIGSKEHLTD MFNMWGQFLI HNMALSKPEP NSWPIKVPKC
DQYFDPACIG NKTMNYFRTR ATEVPCDVGK TVVDEDGKCY EQINSLGSYI DGNVLYGNSE
EICKNLRSLS GGEMKMTVTD VGDLPPKNVP GVPMDNDANL FPIDQLYSVG ERRGNENPGL
LSIHTLLLRD HNRLARKFAR LHPEWDDERV FQQSRSCIIE QIQKITYDEY LPTTLGSFPS
YTGYDANVNA QVSNEFTTTA FRFGHSEVGP FMEYYSENGT RLQPLPIKFS YFNPHALNRG
VEPLIRGLII NEEENIDIYM ISDLRNFLFG KPGQGGLDLA SRNLQRNRDH GIPPYNSLRR
QLGLRPVQTW SDITSDPQIQ NRLKNAYKSV DDIDSYVGGL AEDHMEGSCV GQTFYLIIYE
QFFRTRAGDR FWYETPEMRM VNRECETTTF AEVIKRTTSN IGYVQPNVFR K
