ID   POXA_PENOX              Reviewed;         567 AA.
AC   A0A1W5T3J9;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2017, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=MFS-type tansporter poxA {ECO:0000303|PubMed:28365998};
DE   AltName: Full=Oxaleimides biosynthesis cluster protein A {ECO:0000303|PubMed:28365998};
GN   Name=poxA {ECO:0000303|PubMed:28365998};
OS   Penicillium oxalicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=69781;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RC   STRAIN=K85;
RX   PubMed=28365998; DOI=10.1021/jacs.7b02432;
RA   Sato M., Dander J.E., Sato C., Hung Y.S., Gao S.S., Tang M.C., Hang L.,
RA   Winter J.M., Garg N.K., Watanabe K., Tang Y.;
RT   "Collaborative Biosynthesis of Maleimide- and Succinimide-Containing
RT   Natural Products by Fungal Polyketide Megasynthases.";
RL   J. Am. Chem. Soc. 139:5317-5320(2017).
RN   [2]
RP   FUNCTION.
RX   PubMed=32039410; DOI=10.1039/c9cc09590j;
RA   Hantke V., Skellam E.J., Cox R.J.;
RT   "Evidence for enzyme catalysed intramolecular [4+2] Diels-Alder cyclization
RT   during the biosynthesis of pyrichalasin H.";
RL   Chem. Commun. (Camb.) 56:2925-2928(2020).
CC   -!- FUNCTION: MFS-type transporter; part of the gene cluster that mediates
CC       the biosynthesis of oxaleimides, cytotoxic compounds containing an
CC       unusual disubstituted succinimide moiety.
CC       {ECO:0000269|PubMed:28365998}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:28365998};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- INDUCTION: Expression is positively regulated by the oxaleimides
CC       biosynthesis cluster-specific transcription factor poxB.
CC       {ECO:0000269|PubMed:28365998}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; KY764290; ARF05975.1; -; Genomic_DNA.
DR   SMR; A0A1W5T3J9; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Glycoprotein; Membrane; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..567
FT                   /note="MFS-type tansporter poxA"
FT                   /id="PRO_0000453767"
FT   TRANSMEM        35..55
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        141..161
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        165..185
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        240..260
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        311..331
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        349..369
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        372..392
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        410..430
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        436..456
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        515..535
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          547..567
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        370
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   567 AA;  60410 MW;  60FA7696682AB8F1 CRC64;
     MPASDRTSET GDVEKVTAAE TPKEVPASNA AESTALTGLP LYTVLVGLGL ALFLGAMDMA
     MLGTAVPSIT STFHTTADIG WYGAAYPLTM SSIQLLAGKI YAQFPQKLVF LVFFGLFMLG
     SLLCGVAVNS PMFIVGRATA GAGAAGVLSG TLAIVSAVVP LDKQSLILGL MMSLVGTAVV
     LGPVISGLLT DHSTWRWCFY LNLPCGGVTL LALILFFRPP KRPTRTTPLS IPELIKKLDL
     AGCLGFIPAV VMLLLALQWG GDGSKEHAWN SATIIGLFCG AGVSLILFLI WEHYQGDDAM
     LPLKFFRDLT IIASCLYGFA LLGGYVVVGY FLPEWFQIIK GANPQSSAVM LLPNVITNFI
     SKAVIGVIVN KTGYFNPWLF FGAAVLAIGS GLETNFHPST PRPNWIGYQI LQGAALGIIQ
     APTLGVQVAL AKQKHLIPVA LSLVIFFQYF GSSIMLSISL TIFQNLLRPG LVSKAGMTEA
     QVQQYVAAGN SEVRELTAQI DPSRLGVVLE VYNDAIAGVM WLSTAAALFG FLVSFGFPWK
     SLKAQTEENK KEAAEEEEEV KVAAVEA