POXI_PENO1
ID POXI_PENO1 Reviewed; 314 AA.
AC S8B3I4;
DT 29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT 16-OCT-2013, sequence version 1.
DT 03-AUG-2022, entry version 22.
DE RecName: Full=Oxidoreductase poxI {ECO:0000303|PubMed:28365998};
DE EC=1.3.1.- {ECO:0000305|PubMed:28365998};
DE AltName: Full=Oxaleimides biosynthesis cluster protein I {ECO:0000303|PubMed:28365998};
GN Name=poxI {ECO:0000303|PubMed:28365998}; ORFNames=PDE_04021;
OS Penicillium oxalicum (strain 114-2 / CGMCC 5302) (Penicillium decumbens).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX NCBI_TaxID=933388;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=114-2 / CGMCC 5302;
RX PubMed=23383313; DOI=10.1371/journal.pone.0055185;
RA Liu G., Zhang L., Wei X., Zou G., Qin Y., Ma L., Li J., Zheng H., Wang S.,
RA Wang C., Xun L., Zhao G.-P., Zhou Z., Qu Y.;
RT "Genomic and secretomic analyses reveal unique features of the
RT lignocellulolytic enzyme system of Penicillium decumbens.";
RL PLoS ONE 8:E55185-E55185(2013).
RN [2]
RP FUNCTION, INDUCTION, AND PATHWAY.
RX PubMed=28365998; DOI=10.1021/jacs.7b02432;
RA Sato M., Dander J.E., Sato C., Hung Y.S., Gao S.S., Tang M.C., Hang L.,
RA Winter J.M., Garg N.K., Watanabe K., Tang Y.;
RT "Collaborative Biosynthesis of Maleimide- and Succinimide-Containing
RT Natural Products by Fungal Polyketide Megasynthases.";
RL J. Am. Chem. Soc. 139:5317-5320(2017).
RN [3]
RP FUNCTION.
RX PubMed=32039410; DOI=10.1039/c9cc09590j;
RA Hantke V., Skellam E.J., Cox R.J.;
RT "Evidence for enzyme catalysed intramolecular [4+2] Diels-Alder cyclization
RT during the biosynthesis of pyrichalasin H.";
RL Chem. Commun. (Camb.) 56:2925-2928(2020).
CC -!- FUNCTION: Oxidoreductase; part of the gene cluster that mediates the
CC biosynthesis of oxaleimides, cytotoxic compounds containing an unusual
CC disubstituted succinimide moiety (PubMed:28365998). The first step of
CC the pathway is provided by the HR-PKS poxF that serves in a new mode of
CC collaborative biosynthesis with the PKS-NRPS poxE, by providing the
CC olefin containing amino acid substrate via the synthesis of an ACP-
CC bound dec-4-enoate (PubMed:28365998). The cytochrome P450 monooxygenase
CC poxM-catalyzed oxidation at the alpha-position creates the enzyme-bound
CC 2-hydroxydec-4-enoyl-ACP thioester, which may be prone to spontaneous
CC hydrolysis to yield 2-hydroxydec-4-enoic acid due to increased
CC electrophilicity of the carbonyl (PubMed:28365998). 2-hydroxydec-4-
CC enoic acid can then be further oxidized by poxM to yield the alpha-
CC ketoacid 2-oxodec-4-enoicacid, which is reductively aminated by the
CC aminotransferase poxL to yield (S,E)-2-aminodec-4-enoic acid
CC (PubMed:28365998). The Hybrid PKS-NRPS synthetase poxE then performs
CC condensation between the octaketide product of its PKS modules and the
CC amino group of (S,E)-2-aminodec-4-enoic acid which is activated and
CC incorporated by the adenylation domain (PubMed:28365998). The resulting
CC aminoacyl product can be cyclized by the Diels-Alderase PoxQ and
CC reductively released by the reductive (R) domain of poxE to yield an
CC aldehyde intermediate (PubMed:28365998) (Probable). The released
CC aldehyde is then substrate for a Knoevenagel condensation by the
CC hydrolyase poxO followed by an oxidation at the 5-position of the
CC pyrrolidone ring (PubMed:28365998). The presence of the olefin from the
CC amino acid building block allows for migration of the substituted allyl
CC group to occur (PubMed:28365998). This allylic transposition reaction
CC takes place in a conjugate addition, semipinacol-like fashion to yield
CC a succinimide intermediate (PubMed:28365998). Iterative two-electron
CC oxidations of the C7 methyl of the succinimide intermediate to the
CC carboxylic acid can be catalyzed by one of two remaining cytochrome
CC P450 monooxygenasess poxC or poxD to yield oxaleimide A
CC (PubMed:28365998). Subsequent oxidation yields the maleimide scaffold
CC oxaleimide I (PubMed:28365998). Both oxaleimide A and oxaleimide I can
CC undergo oxidative modifications in the decalin ring to yield the series
CC of products oxaleimides B to H (PubMed:28365998).
CC {ECO:0000269|PubMed:28365998, ECO:0000305|PubMed:32039410}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000305|PubMed:28365998}.
CC -!- INDUCTION: Expression is positively regulated by the oxaleimides
CC biosynthesis cluster-specific transcription factor poxB.
CC {ECO:0000269|PubMed:28365998}.
CC -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family. Isoflavone
CC reductase subfamily. {ECO:0000305}.
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DR EMBL; KB644411; EPS29072.1; -; Genomic_DNA.
DR SMR; S8B3I4; -.
DR EnsemblFungi; EPS29072; EPS29072; PDE_04021.
DR eggNOG; ENOG502SM3M; Eukaryota.
DR HOGENOM; CLU_044876_3_3_1; -.
DR OrthoDB; 1054772at2759; -.
DR PhylomeDB; S8B3I4; -.
DR Proteomes; UP000019376; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd05259; PCBER_SDR_a; 1.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR045312; PCBER-like.
DR Pfam; PF13460; NAD_binding_10; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW NADP; Oxidoreductase; Reference proteome.
FT CHAIN 1..314
FT /note="Oxidoreductase poxI"
FT /id="PRO_0000453772"
SQ SEQUENCE 314 AA; 33846 MW; AEB6D652E7952626 CRC64;
MSTVYYPSVI LKKSPLPLKL TPARASGNFG TPITAALQRA SFNITIITRT ESSSTFPAGL
PIIRTSYTLE NLTTALAGQD AAICVVGPGG IGAQVLMIEA AEAAGVKRFI VDDFGWGPGF
RNLPEFRAIH AHRRAGWELA KAKAQANPNF TFTGITSGNP IDWAMKRFPL MGFDIARCSA
IIYDSGTEKF TATTLAGIGQ SVVGVLQHPD ETANRFVKVL SIITNQNELL EAFQRVTGRQ
WPVQRASAQT LIESGQQKFQ AGMGGWVLEL VVAQMYDEGE ARCVMAPSWE ASDSPLLGVK
KESADEVVAK VLQL
