ID   POXO_PENO1              Reviewed;         421 AA.
AC   S8ASK9;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2013, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Hydrolyase poxO {ECO:0000303|PubMed:28365998};
DE            EC=3.7.1.- {ECO:0000269|PubMed:28365998};
DE   AltName: Full=Oxaleimides biosynthesis cluster protein O {ECO:0000303|PubMed:28365998};
GN   Name=poxO {ECO:0000303|PubMed:28365998}; ORFNames=PDE_04027;
OS   Penicillium oxalicum (strain 114-2 / CGMCC 5302) (Penicillium decumbens).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=933388;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=114-2 / CGMCC 5302;
RX   PubMed=23383313; DOI=10.1371/journal.pone.0055185;
RA   Liu G., Zhang L., Wei X., Zou G., Qin Y., Ma L., Li J., Zheng H., Wang S.,
RA   Wang C., Xun L., Zhao G.-P., Zhou Z., Qu Y.;
RT   "Genomic and secretomic analyses reveal unique features of the
RT   lignocellulolytic enzyme system of Penicillium decumbens.";
RL   PLoS ONE 8:E55185-E55185(2013).
RN   [2]
RP   FUNCTION, INDUCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=28365998; DOI=10.1021/jacs.7b02432;
RA   Sato M., Dander J.E., Sato C., Hung Y.S., Gao S.S., Tang M.C., Hang L.,
RA   Winter J.M., Garg N.K., Watanabe K., Tang Y.;
RT   "Collaborative Biosynthesis of Maleimide- and Succinimide-Containing
RT   Natural Products by Fungal Polyketide Megasynthases.";
RL   J. Am. Chem. Soc. 139:5317-5320(2017).
RN   [3]
RP   FUNCTION.
RX   PubMed=32039410; DOI=10.1039/c9cc09590j;
RA   Hantke V., Skellam E.J., Cox R.J.;
RT   "Evidence for enzyme catalysed intramolecular [4+2] Diels-Alder cyclization
RT   during the biosynthesis of pyrichalasin H.";
RL   Chem. Commun. (Camb.) 56:2925-2928(2020).
CC   -!- FUNCTION: Hydrolyase; part of the gene cluster that mediates the
CC       biosynthesis of oxaleimides, cytotoxic compounds containing an unusual
CC       disubstituted succinimide moiety (PubMed:28365998). The first step of
CC       the pathway is provided by the HR-PKS poxF that serves in a new mode of
CC       collaborative biosynthesis with the PKS-NRPS poxE, by providing the
CC       olefin containing amino acid substrate via the synthesis of an ACP-
CC       bound dec-4-enoate (PubMed:28365998). The cytochrome P450 monooxygenase
CC       poxM-catalyzed oxidation at the alpha-position creates the enzyme-bound
CC       2-hydroxydec-4-enoyl-ACP thioester, which may be prone to spontaneous
CC       hydrolysis to yield 2-hydroxydec-4-enoic acid due to increased
CC       electrophilicity of the carbonyl (PubMed:28365998). 2-hydroxydec-4-
CC       enoic acid can then be further oxidized by poxM to yield the alpha-
CC       ketoacid 2-oxodec-4-enoicacid, which is reductively aminated by the
CC       aminotransferase poxL to yield (S,E)-2-aminodec-4-enoic acid
CC       (PubMed:28365998). The Hybrid PKS-NRPS synthetase poxE then performs
CC       condensation between the octaketide product of its PKS modules and the
CC       amino group of (S,E)-2-aminodec-4-enoic acid which is activated and
CC       incorporated by the adenylation domain (PubMed:28365998). The resulting
CC       aminoacyl product can be cyclized by the Diels-Alderase PoxQ and
CC       reductively released by the reductive (R) domain of poxE to yield an
CC       aldehyde intermediate (PubMed:28365998) (Probable). The released
CC       aldehyde is then substrate for a Knoevenagel condensation by the
CC       hydrolyase poxO followed by an oxidation at the 5-position of the
CC       pyrrolidone ring (PubMed:28365998). The presence of the olefin from the
CC       amino acid building block allows for migration of the substituted allyl
CC       group to occur (PubMed:28365998). This allylic transposition reaction
CC       takes place in a conjugate addition, semipinacol-like fashion to yield
CC       a succinimide intermediate (PubMed:28365998). Iterative two-electron
CC       oxidations of the C7 methyl of the succinimide intermediate to the
CC       carboxylic acid can be catalyzed by one of two remaining cytochrome
CC       P450 monooxygenasess poxC or poxD to yield oxaleimide A
CC       (PubMed:28365998). Subsequent oxidation yields the maleimide scaffold
CC       oxaleimide I (PubMed:28365998). Both oxaleimide A and oxaleimide I can
CC       undergo oxidative modifications in the decalin ring to yield the series
CC       of products oxaleimides B to H (PubMed:28365998).
CC       {ECO:0000269|PubMed:28365998, ECO:0000305|PubMed:32039410}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:28365998}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q93NG6}.
CC   -!- INDUCTION: Expression is positively regulated by the oxaleimides
CC       biosynthesis cluster-specific transcription factor poxB.
CC       {ECO:0000269|PubMed:28365998}.
CC   -!- DISRUPTION PHENOTYPE: Impairs the productin of oxaleimides and leads to
CC       the accumulation of a trans-decalin containing alcohol intermediate.
CC       {ECO:0000269|PubMed:28365998}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. FUS2 hydrolase
CC       family. {ECO:0000305}.
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DR   EMBL; KB644411; EPS29078.1; -; Genomic_DNA.
DR   SMR; S8ASK9; -.
DR   EnsemblFungi; EPS29078; EPS29078; PDE_04027.
DR   eggNOG; ENOG502QPTG; Eukaryota.
DR   HOGENOM; CLU_034451_0_0_1; -.
DR   OrthoDB; 1133794at2759; -.
DR   PhylomeDB; S8ASK9; -.
DR   Proteomes; UP000019376; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR010520; FrsA-like.
DR   Pfam; PF06500; FrsA-like; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..421
FT                   /note="Hydrolyase poxO"
FT                   /id="PRO_0000453778"
FT   ACT_SITE        239
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q4WZB3"
SQ   SEQUENCE   421 AA;  46925 MW;  D44F915CD68974D6 CRC64;
     MHRFFKSEFF NFEFIRILSA APYGGAEIAE CLVAAGQITN DDPESWHRAW IIQADKAKAL
     GDEALHSGDT VSARRAYLRA SNYYRASGYM FHDRPGAPDA RVLPLAQQVL DTYALTLPLL
     DTGEASQLKI PFENYQLAAY LYLPRDRTKP VPVLLSLGGA DSIQEELYYV YAASGPQLGY
     AVLTFEGPGQ GITLRRDKMH MRPDWEVVVG RVLDFLTAYM QQNPSVQLDL SRVAVVGASM
     GGYYALRAAV DPRIGACVSI DPFYDMWDFV RNHVSPALLN AWNAGWVPSR VVNGIMSMAM
     AASFQAKWEV GLAMWFFGVD SPTQTLRHMM KYTLARADGT SRLDQVKCPV LVSGATQSLY
     LEPESDVLRV FDALAHLGDE RREIWIARSP EEGGLQAKIG AIGLVVQRTF RFLDQHLNVT
     R