PP124_ARATH
ID PP124_ARATH Reviewed; 862 AA.
AC Q9S7Q2;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Pentatricopeptide repeat-containing protein At1g74850, chloroplastic;
DE AltName: Full=Protein PLASTID TRANSCRIPTIONALLY ACTIVE 2;
DE Flags: Precursor;
GN Name=PTAC2; OrderedLocusNames=At1g74850; ORFNames=F25A4.18, F9E10.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=16326926; DOI=10.1105/tpc.105.036392;
RA Pfalz J., Liere K., Kandlbinder A., Dietz K.-J., Oelmueller R.;
RT "pTAC2, -6, and -12 are components of the transcriptionally active plastid
RT chromosome that are required for plastid gene expression.";
RL Plant Cell 18:176-197(2006).
CC -!- FUNCTION: Involved in plastid gene expression.
CC {ECO:0000269|PubMed:16326926}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:16326926}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in leaves, stems and flowers, but
CC barely in roots. {ECO:0000269|PubMed:16326926}.
CC -!- DISRUPTION PHENOTYPE: Lethal without exogenous carbon sources. Decrease
CC of total chlorophyll content as well as a decrease in the chlorophyll
CC a:b ratio. Unequally expanded grana thylakoids. Altered expression
CC profiles of plastid genes. {ECO:0000269|PubMed:16326926}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC008263; AAD55291.1; -; Genomic_DNA.
DR EMBL; AC013258; AAG51934.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35639.1; -; Genomic_DNA.
DR PIR; A96778; A96778.
DR RefSeq; NP_177623.1; NM_106143.3.
DR AlphaFoldDB; Q9S7Q2; -.
DR SMR; Q9S7Q2; -.
DR BioGRID; 29043; 10.
DR IntAct; Q9S7Q2; 1.
DR MINT; Q9S7Q2; -.
DR STRING; 3702.AT1G74850.1; -.
DR PaxDb; Q9S7Q2; -.
DR PRIDE; Q9S7Q2; -.
DR ProteomicsDB; 250580; -.
DR EnsemblPlants; AT1G74850.1; AT1G74850.1; AT1G74850.
DR GeneID; 843824; -.
DR Gramene; AT1G74850.1; AT1G74850.1; AT1G74850.
DR KEGG; ath:AT1G74850; -.
DR Araport; AT1G74850; -.
DR TAIR; locus:2027166; AT1G74850.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_49_5_1; -.
DR InParanoid; Q9S7Q2; -.
DR OMA; DVHRMWE; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9S7Q2; -.
DR PRO; PR:Q9S7Q2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S7Q2; baseline and differential.
DR Genevisible; Q9S7Q2; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0042644; C:chloroplast nucleoid; HDA:TAIR.
DR GO; GO:0042793; P:plastid transcription; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 5.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR002625; Smr_dom.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF01535; PPR; 2.
DR Pfam; PF13041; PPR_2; 2.
DR Pfam; PF13812; PPR_3; 3.
DR SMART; SM00463; SMR; 1.
DR TIGRFAMs; TIGR00756; PPR; 11.
DR PROSITE; PS51375; PPR; 16.
DR PROSITE; PS50828; SMR; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Plastid; Reference proteome; Repeat; Transcription;
KW Transcription regulation; Transit peptide.
FT TRANSIT 1..66
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 67..862
FT /note="Pentatricopeptide repeat-containing protein
FT At1g74850, chloroplastic"
FT /id="PRO_0000342865"
FT REPEAT 104..139
FT /note="PPR 1"
FT REPEAT 140..174
FT /note="PPR 2"
FT REPEAT 175..209
FT /note="PPR 3"
FT REPEAT 210..245
FT /note="PPR 4"
FT REPEAT 246..280
FT /note="PPR 5"
FT REPEAT 281..315
FT /note="PPR 6"
FT REPEAT 316..350
FT /note="PPR 7"
FT REPEAT 351..385
FT /note="PPR 8"
FT REPEAT 386..420
FT /note="PPR 9"
FT REPEAT 421..455
FT /note="PPR 10"
FT REPEAT 456..490
FT /note="PPR 11"
FT REPEAT 491..525
FT /note="PPR 12"
FT REPEAT 526..560
FT /note="PPR 13"
FT REPEAT 561..595
FT /note="PPR 14"
FT REPEAT 596..630
FT /note="PPR 15"
FT DOMAIN 713..801
FT /note="Smr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00321"
FT REGION 831..862
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 831..847
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 862 AA; 96326 MW; 674C8853A78751D3 CRC64;
MNLAIPNPNS HHLSFLIQNS SFIGNRRFAD GNRLRFLSGG NRKPCSFSGK IKAKTKDLVL
GNPSVSVEKG KYSYDVESLI NKLSSLPPRG SIARCLDIFK NKLSLNDFAL VFKEFAGRGD
WQRSLRLFKY MQRQIWCKPN EHIYTIMISL LGREGLLDKC LEVFDEMPSQ GVSRSVFSYT
ALINAYGRNG RYETSLELLD RMKNEKISPS ILTYNTVINA CARGGLDWEG LLGLFAEMRH
EGIQPDIVTY NTLLSACAIR GLGDEAEMVF RTMNDGGIVP DLTTYSHLVE TFGKLRRLEK
VCDLLGEMAS GGSLPDITSY NVLLEAYAKS GSIKEAMGVF HQMQAAGCTP NANTYSVLLN
LFGQSGRYDD VRQLFLEMKS SNTDPDAATY NILIEVFGEG GYFKEVVTLF HDMVEENIEP
DMETYEGIIF ACGKGGLHED ARKILQYMTA NDIVPSSKAY TGVIEAFGQA ALYEEALVAF
NTMHEVGSNP SIETFHSLLY SFARGGLVKE SEAILSRLVD SGIPRNRDTF NAQIEAYKQG
GKFEEAVKTY VDMEKSRCDP DERTLEAVLS VYSFARLVDE CREQFEEMKA SDILPSIMCY
CMMLAVYGKT ERWDDVNELL EEMLSNRVSN IHQVIGQMIK GDYDDDSNWQ IVEYVLDKLN
SEGCGLGIRF YNALLDALWW LGQKERAARV LNEATKRGLF PELFRKNKLV WSVDVHRMSE
GGMYTALSVW LNDINDMLLK GDLPQLAVVV SVRGQLEKSS AARESPIAKA AFSFLQDHVS
SSFSFTGWNG GRIMCQRSQL KQLLSTKEPT SEESENKNLV ALANSPIFAA GTRASTSSDT
NHSGNPTQRR TRTKKELAGS TA
