PP125_ARATH
ID PP125_ARATH Reviewed; 482 AA.
AC Q9S7R4; A4FVT1;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Pentatricopeptide repeat-containing protein At1g74900, mitochondrial;
DE AltName: Full=Protein ORGANELLE TRANSCRIPT PROCESSING DEFECT 43;
DE Flags: Precursor;
GN Name=OTP43; OrderedLocusNames=At1g74900; ORFNames=F25A4.13, F9E10.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [5]
RP FUNCTION.
RX PubMed=17965268; DOI=10.1105/tpc.107.054841;
RA de Longevialle A.F., Meyer E.H., Andres C., Taylor N.L., Lurin C.,
RA Millar A.H., Small I.D.;
RT "The pentatricopeptide repeat gene OTP43 is required for trans-splicing of
RT the mitochondrial nad1 intron 1 in Arabidopsis thaliana.";
RL Plant Cell 19:3256-3265(2007).
CC -!- FUNCTION: Required for the trans-splicing of intron 1 of the
CC mitochondrial nad1 transcript encoding the ND1 subunit of the
CC mitochondrial membrane respiratory chain NADH dehydrogenase (Complex
CC I). {ECO:0000269|PubMed:17965268}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9S7R4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9S7R4-2; Sequence=VSP_034551;
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC008263; AAD55286.1; -; Genomic_DNA.
DR EMBL; AC013258; AAG51911.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35645.1; -; Genomic_DNA.
DR EMBL; BT030379; ABO38792.1; -; mRNA.
DR PIR; F96778; F96778.
DR RefSeq; NP_177628.2; NM_106148.2. [Q9S7R4-2]
DR AlphaFoldDB; Q9S7R4; -.
DR SMR; Q9S7R4; -.
DR STRING; 3702.AT1G74900.1; -.
DR PRIDE; Q9S7R4; -.
DR EnsemblPlants; AT1G74900.1; AT1G74900.1; AT1G74900. [Q9S7R4-2]
DR GeneID; 843829; -.
DR Gramene; AT1G74900.1; AT1G74900.1; AT1G74900. [Q9S7R4-2]
DR KEGG; ath:AT1G74900; -.
DR Araport; AT1G74900; -.
DR TAIR; locus:2027222; AT1G74900.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_49_20_1; -.
DR InParanoid; Q9S7R4; -.
DR OMA; CPRQIAA; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9S7R4; -.
DR PRO; PR:Q9S7R4; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S7R4; baseline and differential.
DR Genevisible; Q9S7R4; AT.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0008380; P:RNA splicing; IGI:TAIR.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF01535; PPR; 1.
DR Pfam; PF12854; PPR_1; 1.
DR Pfam; PF13041; PPR_2; 3.
DR TIGRFAMs; TIGR00756; PPR; 8.
DR PROSITE; PS51375; PPR; 11.
PE 2: Evidence at transcript level;
KW Alternative splicing; Mitochondrion; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..482
FT /note="Pentatricopeptide repeat-containing protein
FT At1g74900, mitochondrial"
FT /id="PRO_0000342866"
FT REPEAT 90..124
FT /note="PPR 1"
FT REPEAT 125..159
FT /note="PPR 2"
FT REPEAT 160..190
FT /note="PPR 3"
FT REPEAT 194..228
FT /note="PPR 4"
FT REPEAT 229..263
FT /note="PPR 5"
FT REPEAT 264..298
FT /note="PPR 6"
FT REPEAT 299..333
FT /note="PPR 7"
FT REPEAT 334..368
FT /note="PPR 8"
FT REPEAT 369..403
FT /note="PPR 9"
FT REPEAT 404..441
FT /note="PPR 10"
FT REPEAT 442..476
FT /note="PPR 11"
FT VAR_SEQ 429..457
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_034551"
SQ SEQUENCE 482 AA; 55082 MW; 5C7CFFF96484B32F CRC64;
MKRLFSKSLC TSAAGANLKP PPADSAAIAK LILSSPNTTH QDDQFLLSTK TTPWTPNLVN
SVLKRLWNHG PKALQFFHFL DNHHREYVHD ASSFDLAIDI AARLHLHPTV WSLIHRMRSL
RIGPSPKTFA IVAERYASAG KPDKAVKLFL NMHEHGCFQD LASFNTILDV LCKSKRVEKA
YELFRALRGR FSVDTVTYNV ILNGWCLIKR TPKALEVLKE MVERGINPNL TTYNTMLKGF
FRAGQIRHAW EFFLEMKKRD CEIDVVTYTT VVHGFGVAGE IKRARNVFDE MIREGVLPSV
ATYNAMIQVL CKKDNVENAV VMFEEMVRRG YEPNVTTYNV LIRGLFHAGE FSRGEELMQR
MENEGCEPNF QTYNMMIRYY SECSEVEKAL GLFEKMGSGD CLPNLDTYNI LISGMFVRKR
SEDMVVAGKL LLEMVERGFI PRKFTFNRVL NGLLLTGNQA FAKEILRLQS KSGSRLLRKF
RL
