PP14A_HUMAN
ID PP14A_HUMAN Reviewed; 147 AA.
AC Q96A00; Q7Z4X7; Q96S54;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 14A;
DE AltName: Full=17 kDa PKC-potentiated inhibitory protein of PP1;
DE AltName: Full=Protein kinase C-potentiated inhibitor protein of 17 kDa;
DE Short=CPI-17;
GN Name=PPP1R14A; Synonyms=CPI17, PPP1INL;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RC TISSUE=Aorta;
RX PubMed=11467857; DOI=10.1006/bbrc.2001.5290;
RA Yamawaki K., Ito M., Machida H., Moriki N., Okamoto R., Isaka N.,
RA Shimpo H., Kohda A., Okumura K., Hartshorne D.J., Nakano T.;
RT "Identification of human CPI-17, an inhibitory phosphoprotein for myosin
RT phosphatase.";
RL Biochem. Biophys. Res. Commun. 285:1040-1045(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Cui W.C., Yu L., Zhao S.Y.;
RT "Cloning a new human cDNA homologous to Sus scrofa mRNA for 17-KDa PKC-
RT potentiated inhibitory protein of PP1.";
RL Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
RC TISSUE=Smooth muscle;
RX PubMed=12974676; DOI=10.1042/bj20030128;
RA Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J.,
RA Uhl G.R.;
RT "GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein,
RT is activated by PKC and inactivated by PKA.";
RL Biochem. J. 377:171-181(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Pancreas;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION AT THR-38.
RX PubMed=15003508; DOI=10.1016/j.bbrc.2004.02.014;
RA Zemlickova E., Johannes F.J., Aitken A., Dubois T.;
RT "Association of CPI-17 with protein kinase C and casein kinase I.";
RL Biochem. Biophys. Res. Commun. 316:39-47(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Platelet;
RX PubMed=18088087; DOI=10.1021/pr0704130;
RA Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J.,
RA Schuetz C., Walter U., Gambaryan S., Sickmann A.;
RT "Phosphoproteome of resting human platelets.";
RL J. Proteome Res. 7:526-534(2008).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21406692; DOI=10.1126/scisignal.2001570;
RA Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T.,
RA Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.;
RT "System-wide temporal characterization of the proteome and phosphoproteome
RT of human embryonic stem cell differentiation.";
RL Sci. Signal. 4:RS3-RS3(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-128; SER-134 AND
RP SER-136, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- FUNCTION: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory
CC activity when phosphorylated, creating a molecular switch for
CC regulating the phosphorylation status of PPP1CA substrates and smooth
CC muscle contraction.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=CPI-17alpha;
CC IsoId=Q96A00-1; Sequence=Displayed;
CC Name=2; Synonyms=CPI-17beta;
CC IsoId=Q96A00-2; Sequence=VSP_011841;
CC -!- TISSUE SPECIFICITY: Isoform 1 is detected in aorta and testis. Isoform
CC 2 is detected in aorta. {ECO:0000269|PubMed:11467857}.
CC -!- SIMILARITY: Belongs to the PP1 inhibitor family. {ECO:0000305}.
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DR EMBL; AB056508; BAB62502.1; -; mRNA.
DR EMBL; AB056509; BAB62503.1; -; mRNA.
DR EMBL; AF123763; AAP97242.1; -; mRNA.
DR EMBL; AY050668; AAL24445.1; -; Genomic_DNA.
DR EMBL; AY050670; AAL25828.1; -; mRNA.
DR EMBL; BC021089; AAH21089.1; -; mRNA.
DR CCDS; CCDS12509.1; -. [Q96A00-1]
DR CCDS; CCDS58660.1; -. [Q96A00-2]
DR PIR; JC7719; JC7719.
DR RefSeq; NP_001230876.1; NM_001243947.1. [Q96A00-2]
DR RefSeq; NP_150281.1; NM_033256.2. [Q96A00-1]
DR AlphaFoldDB; Q96A00; -.
DR SMR; Q96A00; -.
DR BioGRID; 125153; 23.
DR IntAct; Q96A00; 2.
DR MINT; Q96A00; -.
DR STRING; 9606.ENSP00000301242; -.
DR iPTMnet; Q96A00; -.
DR PhosphoSitePlus; Q96A00; -.
DR BioMuta; PPP1R14A; -.
DR DMDM; 55583974; -.
DR EPD; Q96A00; -.
DR jPOST; Q96A00; -.
DR MassIVE; Q96A00; -.
DR MaxQB; Q96A00; -.
DR PaxDb; Q96A00; -.
DR PeptideAtlas; Q96A00; -.
DR PRIDE; Q96A00; -.
DR ProteomicsDB; 75885; -. [Q96A00-1]
DR ProteomicsDB; 75886; -. [Q96A00-2]
DR Antibodypedia; 3563; 390 antibodies from 35 providers.
DR DNASU; 94274; -.
DR Ensembl; ENST00000301242.9; ENSP00000301242.3; ENSG00000167641.11. [Q96A00-1]
DR Ensembl; ENST00000347262.8; ENSP00000301243.3; ENSG00000167641.11. [Q96A00-2]
DR GeneID; 94274; -.
DR KEGG; hsa:94274; -.
DR MANE-Select; ENST00000301242.9; ENSP00000301242.3; NM_033256.3; NP_150281.1.
DR UCSC; uc002ohq.3; human. [Q96A00-1]
DR CTD; 94274; -.
DR DisGeNET; 94274; -.
DR GeneCards; PPP1R14A; -.
DR HGNC; HGNC:14871; PPP1R14A.
DR HPA; ENSG00000167641; Tissue enhanced (brain).
DR MIM; 608153; gene.
DR neXtProt; NX_Q96A00; -.
DR OpenTargets; ENSG00000167641; -.
DR PharmGKB; PA33623; -.
DR VEuPathDB; HostDB:ENSG00000167641; -.
DR eggNOG; ENOG502S2I4; Eukaryota.
DR GeneTree; ENSGT00950000182985; -.
DR HOGENOM; CLU_114155_2_0_1; -.
DR InParanoid; Q96A00; -.
DR OMA; LHKQPGF; -.
DR OrthoDB; 1492626at2759; -.
DR PhylomeDB; Q96A00; -.
DR TreeFam; TF105546; -.
DR PathwayCommons; Q96A00; -.
DR Reactome; R-HSA-5625740; RHO GTPases activate PKNs.
DR SignaLink; Q96A00; -.
DR SIGNOR; Q96A00; -.
DR BioGRID-ORCS; 94274; 13 hits in 1081 CRISPR screens.
DR ChiTaRS; PPP1R14A; human.
DR GeneWiki; PPP1R14A; -.
DR GenomeRNAi; 94274; -.
DR Pharos; Q96A00; Tbio.
DR PRO; PR:Q96A00; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q96A00; protein.
DR Bgee; ENSG00000167641; Expressed in right coronary artery and 147 other tissues.
DR ExpressionAtlas; Q96A00; baseline and differential.
DR Genevisible; Q96A00; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR GO; GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
DR Gene3D; 1.10.150.220; -; 1.
DR InterPro; IPR008025; CPI-17.
DR InterPro; IPR036658; CPI-17_sf.
DR PANTHER; PTHR16188; PTHR16188; 1.
DR Pfam; PF05361; PP1_inhibitor; 1.
DR SUPFAM; SSF81790; SSF81790; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein;
KW Protein phosphatase inhibitor; Reference proteome.
FT CHAIN 1..147
FT /note="Protein phosphatase 1 regulatory subunit 14A"
FT /id="PRO_0000071486"
FT REGION 1..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 35..120
FT /note="Inhibitory"
FT REGION 118..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18088087,
FT ECO:0007744|PubMed:23186163"
FT MOD_RES 38
FT /note="Phosphothreonine; by PKC"
FT /evidence="ECO:0000269|PubMed:15003508"
FT MOD_RES 128
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 134
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 136
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21406692,
FT ECO:0007744|PubMed:23186163"
FT VAR_SEQ 68..94
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:11467857"
FT /id="VSP_011841"
FT CONFLICT 123
FT /note="G -> R (in Ref. 2; AAP97242)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 147 AA; 16693 MW; 470EE2C082FF7319 CRC64;
MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL
EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR
QPGLRQPSPS HDGSLSPLQD RARTAHP
