PP14A_MOUSE
ID PP14A_MOUSE Reviewed; 147 AA.
AC Q91VC7; Q99MB9;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 14A;
DE AltName: Full=17 kDa PKC-potentiated inhibitory protein of PP1;
DE AltName: Full=Protein kinase C-potentiated inhibitor protein of 17 kDa;
DE Short=CPI-17;
GN Name=Ppp1r14a; Synonyms=Cpi17;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Smooth muscle;
RX PubMed=12974676; DOI=10.1042/bj20030128;
RA Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J.,
RA Uhl G.R.;
RT "GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein,
RT is activated by PKC and inactivated by PKA.";
RL Biochem. J. 377:171-181(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yu L.;
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-136, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory
CC activity when phosphorylated, creating a molecular switch for
CC regulating the phosphorylation status of PPP1CA substrates and smooth
CC muscle contraction (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PP1 inhibitor family. {ECO:0000305}.
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DR EMBL; AY050672; AAL25830.1; -; mRNA.
DR EMBL; AF352573; AAK35214.1; -; mRNA.
DR EMBL; BC010832; AAH10832.1; -; mRNA.
DR CCDS; CCDS21070.1; -.
DR RefSeq; NP_081007.2; NM_026731.3.
DR AlphaFoldDB; Q91VC7; -.
DR SMR; Q91VC7; -.
DR BioGRID; 212861; 4.
DR STRING; 10090.ENSMUSP00000035642; -.
DR iPTMnet; Q91VC7; -.
DR PhosphoSitePlus; Q91VC7; -.
DR MaxQB; Q91VC7; -.
DR PaxDb; Q91VC7; -.
DR PeptideAtlas; Q91VC7; -.
DR PRIDE; Q91VC7; -.
DR ProteomicsDB; 291773; -.
DR Antibodypedia; 3563; 390 antibodies from 35 providers.
DR DNASU; 68458; -.
DR Ensembl; ENSMUST00000048187; ENSMUSP00000035642; ENSMUSG00000037166.
DR GeneID; 68458; -.
DR KEGG; mmu:68458; -.
DR UCSC; uc009gbn.1; mouse.
DR CTD; 94274; -.
DR MGI; MGI:1931139; Ppp1r14a.
DR VEuPathDB; HostDB:ENSMUSG00000037166; -.
DR eggNOG; ENOG502S2I4; Eukaryota.
DR GeneTree; ENSGT00950000182985; -.
DR HOGENOM; CLU_114155_2_0_1; -.
DR InParanoid; Q91VC7; -.
DR OMA; LHKQPGF; -.
DR OrthoDB; 1492626at2759; -.
DR PhylomeDB; Q91VC7; -.
DR TreeFam; TF105546; -.
DR Reactome; R-MMU-5625740; RHO GTPases activate PKNs.
DR BioGRID-ORCS; 68458; 2 hits in 74 CRISPR screens.
DR ChiTaRS; Ppp1r14a; mouse.
DR PRO; PR:Q91VC7; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q91VC7; protein.
DR Bgee; ENSMUSG00000037166; Expressed in aorta tunica media and 184 other tissues.
DR ExpressionAtlas; Q91VC7; baseline and differential.
DR Genevisible; Q91VC7; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IDA:MGI.
DR GO; GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
DR Gene3D; 1.10.150.220; -; 1.
DR InterPro; IPR008025; CPI-17.
DR InterPro; IPR036658; CPI-17_sf.
DR PANTHER; PTHR16188; PTHR16188; 1.
DR Pfam; PF05361; PP1_inhibitor; 1.
DR SUPFAM; SSF81790; SSF81790; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Protein phosphatase inhibitor;
KW Reference proteome.
FT CHAIN 1..147
FT /note="Protein phosphatase 1 regulatory subunit 14A"
FT /id="PRO_0000071487"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 35..120
FT /note="Inhibitory"
FT REGION 118..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 38
FT /note="Phosphothreonine; by PKC"
FT /evidence="ECO:0000250|UniProtKB:Q96A00"
FT MOD_RES 128
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96A00"
FT MOD_RES 134
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96A00"
FT MOD_RES 136
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 113
FT /note="A -> G (in Ref. 2; AAK35214)"
FT /evidence="ECO:0000305"
FT CONFLICT 135
FT /note="L -> P (in Ref. 2; AAK35214)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 147 AA; 16649 MW; 1E29065ACEB39C4D CRC64;
MAAQRLGKRV LSKLQSPSRA RGPGGSPSGL QKRHARVTVK YDRRELQRRL DVEKWIDGCL
EELYRGRESD MPDEVNIDEL LELDSEEERC RKIQGLLEAC ANPTEDFVQE LLAKLRGLHK
QPGFPQPSPS DDPSLSPRQD RAHTAPP
