PP14B_MOUSE
ID PP14B_MOUSE Reviewed; 147 AA.
AC Q62084; Q640Q7;
DT 09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 09-NOV-2004, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 14B;
DE AltName: Full=Phosphatase holoenzyme inhibitor 1;
DE Short=PHI-1;
DE AltName: Full=Phospholipase C-beta-3 neighbouring gene protein;
GN Name=Ppp1r14b; Synonyms=Png;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=8670283; DOI=10.1006/bbrc.1996.0895;
RA Lagercrantz J., Kedra D., Carson E., Nordenskjoeld M., Dumanski J.P.,
RA Weber G., Piehl F.;
RT "Sequence and expression of the mouse homologue to human phospholipase C
RT beta3 neighboring gene.";
RL Biochem. Biophys. Res. Commun. 223:335-340(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, MUTAGENESIS OF THR-57, PHOSPHORYLATION AT THR-57, AND TISSUE
RP SPECIFICITY.
RX PubMed=10606530; DOI=10.1021/bi992030o;
RA Eto M., Karginov A., Brautigan D.L.;
RT "A novel phosphoprotein inhibitor of protein type-1 phosphatase
RT holoenzymes.";
RL Biochemistry 38:16952-16957(1999).
RN [4]
RP FUNCTION, AND PHOSPHORYLATION AT THR-57.
RX PubMed=12144526; DOI=10.1042/bj20020522;
RA Deng J.T., Sutherland C., Brautigan D.L., Eto M., Walsh M.P.;
RT "Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by
RT integrin-linked kinase.";
RL Biochem. J. 367:517-524(2002).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Inhibitor of PPP1CA. Has over 50-fold higher inhibitory
CC activity when phosphorylated. {ECO:0000269|PubMed:10606530,
CC ECO:0000269|PubMed:12144526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Ubiquitous. Highly expressed in testis. Detected at
CC low levels in the other tissues tested. Highly expressed in cardiac
CC muscle, bladder and aorta (at protein level).
CC {ECO:0000269|PubMed:10606530, ECO:0000269|PubMed:8670283}.
CC -!- PTM: Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown
CC Ser is also phosphorylated by PKC (in vitro).
CC {ECO:0000269|PubMed:10606530, ECO:0000269|PubMed:12144526}.
CC -!- SIMILARITY: Belongs to the PP1 inhibitor family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA66121.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X97490; CAA66121.1; ALT_INIT; mRNA.
DR EMBL; BC082545; AAH82545.2; -; mRNA.
DR EMBL; BC125650; AAI25651.1; -; mRNA.
DR EMBL; BC125654; AAI25655.1; -; mRNA.
DR CCDS; CCDS29514.1; -.
DR PIR; JC4871; JC4871.
DR RefSeq; NP_032915.2; NM_008889.2.
DR AlphaFoldDB; Q62084; -.
DR BioGRID; 202273; 3.
DR STRING; 10090.ENSMUSP00000064129; -.
DR iPTMnet; Q62084; -.
DR PhosphoSitePlus; Q62084; -.
DR EPD; Q62084; -.
DR MaxQB; Q62084; -.
DR PaxDb; Q62084; -.
DR PeptideAtlas; Q62084; -.
DR PRIDE; Q62084; -.
DR ProteomicsDB; 289868; -.
DR Ensembl; ENSMUST00000070850; ENSMUSP00000064129; ENSMUSG00000056612.
DR GeneID; 18938; -.
DR KEGG; mmu:18938; -.
DR UCSC; uc008gjs.1; mouse.
DR CTD; 26472; -.
DR MGI; MGI:107682; Ppp1r14b.
DR VEuPathDB; HostDB:ENSMUSG00000056612; -.
DR eggNOG; KOG0824; Eukaryota.
DR GeneTree; ENSGT00950000182985; -.
DR HOGENOM; CLU_114155_1_0_1; -.
DR InParanoid; Q62084; -.
DR OMA; TQLYDCQ; -.
DR OrthoDB; 1492626at2759; -.
DR PhylomeDB; Q62084; -.
DR TreeFam; TF105546; -.
DR BioGRID-ORCS; 18938; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Ppp1r14b; mouse.
DR PRO; PR:Q62084; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q62084; protein.
DR Bgee; ENSMUSG00000056612; Expressed in cortical plate and 88 other tissues.
DR ExpressionAtlas; Q62084; baseline and differential.
DR Genevisible; Q62084; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0019212; F:phosphatase inhibitor activity; ISO:MGI.
DR GO; GO:0019888; F:protein phosphatase regulator activity; ISO:MGI.
DR GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR GO; GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
DR Gene3D; 1.10.150.220; -; 1.
DR InterPro; IPR008025; CPI-17.
DR InterPro; IPR036658; CPI-17_sf.
DR PANTHER; PTHR16188; PTHR16188; 1.
DR Pfam; PF05361; PP1_inhibitor; 1.
DR SUPFAM; SSF81790; SSF81790; 1.
PE 1: Evidence at protein level;
KW Acetylation; Coiled coil; Cytoplasm; Phosphoprotein;
KW Protein phosphatase inhibitor; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q96C90"
FT CHAIN 2..147
FT /note="Protein phosphatase 1 regulatory subunit 14B"
FT /id="PRO_0000071491"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 61..103
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q96C90"
FT MOD_RES 21
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96C90"
FT MOD_RES 29
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q96C90"
FT MOD_RES 32
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96C90"
FT MOD_RES 57
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:10606530,
FT ECO:0000269|PubMed:12144526"
FT MUTAGEN 57
FT /note="T->A: Abolishes phosphorylation and strongly reduces
FT inhibitory activity."
FT /evidence="ECO:0000269|PubMed:10606530"
SQ SEQUENCE 147 AA; 15957 MW; D1E0383A3A9DF822 CRC64;
MADSGPAGGA ALAAPAPGPG SGSTGPRVYF QSPPGAAGEG PGGADDDGPV RRQGKVTVKY
DRKELRKRLN LEEWILEQLT RLYDCQEEEI PELEIDVDEL LDMESDDTRA ARVKELLVDC
YKPTEAFISG LLDKIRGMQK LSTPQKK
