ID   PP14B_PIG               Reviewed;         147 AA.
AC   Q8MIK9;
DT   09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Protein phosphatase 1 regulatory subunit 14B;
DE   AltName: Full=Phosphatase holoenzyme inhibitor 1;
DE            Short=PHI-1;
DE   AltName: Full=Ubiquitous PKC-potentiated PP1 inhibitor;
GN   Name=PPP1R14B; Synonyms=PHI1;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12974676; DOI=10.1042/bj20030128;
RA   Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J.,
RA   Uhl G.R.;
RT   "GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein,
RT   is activated by PKC and inactivated by PKA.";
RL   Biochem. J. 377:171-181(2004).
CC   -!- FUNCTION: Inhibitor of PPP1CA. Has over 50-fold higher inhibitory
CC       activity when phosphorylated (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown
CC       Ser is also phosphorylated by PKC (in vitro) (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PP1 inhibitor family. {ECO:0000305}.
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DR   EMBL; AY122324; AAM89293.1; -; mRNA.
DR   RefSeq; NP_999280.1; NM_214115.1.
DR   AlphaFoldDB; Q8MIK9; -.
DR   STRING; 9823.ENSSSCP00000013862; -.
DR   PaxDb; Q8MIK9; -.
DR   PeptideAtlas; Q8MIK9; -.
DR   Ensembl; ENSSSCT00000056362; ENSSSCP00000035611; ENSSSCG00000037999.
DR   Ensembl; ENSSSCT00025106307; ENSSSCP00025047694; ENSSSCG00025076692.
DR   Ensembl; ENSSSCT00030018541; ENSSSCP00030008217; ENSSSCG00030013509.
DR   Ensembl; ENSSSCT00035111297; ENSSSCP00035048717; ENSSSCG00035081047.
DR   Ensembl; ENSSSCT00040071237; ENSSSCP00040030384; ENSSSCG00040052680.
DR   Ensembl; ENSSSCT00045050098; ENSSSCP00045034856; ENSSSCG00045029277.
DR   Ensembl; ENSSSCT00050048170; ENSSSCP00050020039; ENSSSCG00050035824.
DR   Ensembl; ENSSSCT00055003887; ENSSSCP00055002965; ENSSSCG00055002057.
DR   Ensembl; ENSSSCT00060039398; ENSSSCP00060016699; ENSSSCG00060029154.
DR   Ensembl; ENSSSCT00065014286; ENSSSCP00065005816; ENSSSCG00065010749.
DR   Ensembl; ENSSSCT00070047663; ENSSSCP00070040225; ENSSSCG00070023829.
DR   GeneID; 397205; -.
DR   KEGG; ssc:397205; -.
DR   CTD; 26472; -.
DR   VGNC; VGNC:91723; PPP1R14B.
DR   eggNOG; KOG0824; Eukaryota.
DR   GeneTree; ENSGT00950000182985; -.
DR   HOGENOM; CLU_114155_1_0_1; -.
DR   InParanoid; Q8MIK9; -.
DR   OMA; CGVETMN; -.
DR   OrthoDB; 1492626at2759; -.
DR   TreeFam; TF105546; -.
DR   Proteomes; UP000008227; Chromosome 2.
DR   Proteomes; UP000314985; Chromosome 2.
DR   Bgee; ENSSSCG00000037999; Expressed in hindlimb bud and 47 other tissues.
DR   ExpressionAtlas; Q8MIK9; baseline and differential.
DR   Genevisible; Q8MIK9; SS.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   GO; GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
DR   Gene3D; 1.10.150.220; -; 1.
DR   InterPro; IPR008025; CPI-17.
DR   InterPro; IPR036658; CPI-17_sf.
DR   PANTHER; PTHR16188; PTHR16188; 1.
DR   Pfam; PF05361; PP1_inhibitor; 1.
DR   SUPFAM; SSF81790; SSF81790; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Coiled coil; Cytoplasm; Phosphoprotein;
KW   Protein phosphatase inhibitor; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   CHAIN           2..147
FT                   /note="Protein phosphatase 1 regulatory subunit 14B"
FT                   /id="PRO_0000071492"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          61..103
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         21
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         29
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         32
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         57
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q62084"
SQ   SEQUENCE   147 AA;  15911 MW;  C4E805E642E25D89 CRC64;
     MADSGPAGGA ALAAPAPGPG SGGAGPRVYF QSPPGAAGEG PGGADDEGPV RRQGKVTVKY
     DRKELRKRLN LEEWILEQLT RLYDCQEEEI PELEIDVDEL LDMESDDTRA ARVKELLVDC
     YKPTEAFISG LLDKIRGMQK LSTPQKK