ID   PP14B_RAT               Reviewed;         147 AA.
AC   Q8K3F3;
DT   09-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Protein phosphatase 1 regulatory subunit 14B;
DE   AltName: Full=Phosphatase holoenzyme inhibitor 1;
DE            Short=PHI-1;
DE   AltName: Full=Ubiquitous PKC-potentiated PP1 inhibitor;
GN   Name=Ppp1r14b; Synonyms=Phi1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RX   PubMed=12974676; DOI=10.1042/bj20030128;
RA   Liu Q.-R., Zhang P.-W., Lin Z., Li Q.-F., Woods A.S., Troncoso J.,
RA   Uhl G.R.;
RT   "GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein,
RT   is activated by PKC and inactivated by PKA.";
RL   Biochem. J. 377:171-181(2004).
CC   -!- FUNCTION: Inhibitor of PPP1CA. Has over 50-fold higher inhibitory
CC       activity when phosphorylated (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated primarily on Thr-57 by PKC (in vitro). An unknown
CC       Ser is also phosphorylated by PKC (in vitro) (By similarity).
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PP1 inhibitor family. {ECO:0000305}.
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DR   EMBL; AY122323; AAM89292.1; -; mRNA.
DR   RefSeq; NP_742042.1; NM_172045.1.
DR   AlphaFoldDB; Q8K3F3; -.
DR   STRING; 10116.ENSRNOP00000040750; -.
DR   jPOST; Q8K3F3; -.
DR   PaxDb; Q8K3F3; -.
DR   PRIDE; Q8K3F3; -.
DR   GeneID; 259225; -.
DR   KEGG; rno:259225; -.
DR   CTD; 26472; -.
DR   RGD; 628702; Ppp1r14b.
DR   VEuPathDB; HostDB:ENSRNOG00000021151; -.
DR   eggNOG; KOG0824; Eukaryota.
DR   HOGENOM; CLU_114155_1_0_1; -.
DR   InParanoid; Q8K3F3; -.
DR   OMA; TQLYDCQ; -.
DR   OrthoDB; 1492626at2759; -.
DR   PhylomeDB; Q8K3F3; -.
DR   TreeFam; TF105546; -.
DR   PRO; PR:Q8K3F3; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Bgee; ENSRNOG00000021151; Expressed in thymus and 20 other tissues.
DR   Genevisible; Q8K3F3; RN.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019212; F:phosphatase inhibitor activity; IDA:RGD.
DR   GO; GO:0019888; F:protein phosphatase regulator activity; IDA:RGD.
DR   GO; GO:0004865; F:protein serine/threonine phosphatase inhibitor activity; IBA:GO_Central.
DR   GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR   GO; GO:0042325; P:regulation of phosphorylation; IEA:InterPro.
DR   Gene3D; 1.10.150.220; -; 1.
DR   InterPro; IPR008025; CPI-17.
DR   InterPro; IPR036658; CPI-17_sf.
DR   PANTHER; PTHR16188; PTHR16188; 1.
DR   Pfam; PF05361; PP1_inhibitor; 1.
DR   SUPFAM; SSF81790; SSF81790; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Coiled coil; Cytoplasm; Phosphoprotein;
KW   Protein phosphatase inhibitor; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   CHAIN           2..147
FT                   /note="Protein phosphatase 1 regulatory subunit 14B"
FT                   /id="PRO_0000071493"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          61..103
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         21
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         29
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         32
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q96C90"
FT   MOD_RES         57
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q62084"
SQ   SEQUENCE   147 AA;  15957 MW;  D1E0383A3A9DF822 CRC64;
     MADSGPAGGA ALAAPAPGPG SGSTGPRVYF QSPPGAAGEG PGGADDDGPV RRQGKVTVKY
     DRKELRKRLN LEEWILEQLT RLYDCQEEEI PELEIDVDEL LDMESDDTRA ARVKELLVDC
     YKPTEAFISG LLDKIRGMQK LSTPQKK