PP166_ARATH
ID PP166_ARATH Reviewed; 490 AA.
AC Q9SKU6; Q3EBX7;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Pentatricopeptide repeat-containing protein At2g20710, mitochondrial;
DE Flags: Precursor;
GN OrderedLocusNames=At2g20710; ORFNames=F5H14.32;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (AUG-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9SKU6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9SKU6-2; Sequence=VSP_035969;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX819495; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC006234; AAD20924.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07059.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07060.1; -; Genomic_DNA.
DR EMBL; BX819495; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BT015322; AAT99801.1; -; mRNA.
DR EMBL; BT029763; ABM06033.1; -; mRNA.
DR PIR; D84592; D84592.
DR RefSeq; NP_179663.1; NM_127635.4. [Q9SKU6-1]
DR RefSeq; NP_973492.1; NM_201763.2. [Q9SKU6-2]
DR AlphaFoldDB; Q9SKU6; -.
DR SMR; Q9SKU6; -.
DR STRING; 3702.AT2G20710.1; -.
DR PaxDb; Q9SKU6; -.
DR PRIDE; Q9SKU6; -.
DR ProteomicsDB; 250496; -. [Q9SKU6-1]
DR EnsemblPlants; AT2G20710.1; AT2G20710.1; AT2G20710. [Q9SKU6-1]
DR EnsemblPlants; AT2G20710.2; AT2G20710.2; AT2G20710. [Q9SKU6-2]
DR GeneID; 816599; -.
DR Gramene; AT2G20710.1; AT2G20710.1; AT2G20710. [Q9SKU6-1]
DR Gramene; AT2G20710.2; AT2G20710.2; AT2G20710. [Q9SKU6-2]
DR KEGG; ath:AT2G20710; -.
DR Araport; AT2G20710; -.
DR TAIR; locus:2051379; AT2G20710.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_019802_3_0_1; -.
DR InParanoid; Q9SKU6; -.
DR OMA; ASKPGWK; -.
DR OrthoDB; 670116at2759; -.
DR PhylomeDB; Q9SKU6; -.
DR PRO; PR:Q9SKU6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SKU6; baseline and differential.
DR Genevisible; Q9SKU6; AT.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF01535; PPR; 4.
DR Pfam; PF13041; PPR_2; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR TIGRFAMs; TIGR00756; PPR; 3.
DR PROSITE; PS51375; PPR; 9.
PE 2: Evidence at transcript level;
KW Alternative splicing; Mitochondrion; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..86
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 87..490
FT /note="Pentatricopeptide repeat-containing protein
FT At2g20710, mitochondrial"
FT /id="PRO_0000356025"
FT REPEAT 138..172
FT /note="PPR 1"
FT REPEAT 173..207
FT /note="PPR 2"
FT REPEAT 208..243
FT /note="PPR 3"
FT REPEAT 244..274
FT /note="PPR 4"
FT REPEAT 280..310
FT /note="PPR 5"
FT REPEAT 314..344
FT /note="PPR 6"
FT REPEAT 349..379
FT /note="PPR 7"
FT REPEAT 384..421
FT /note="PPR 8"
FT VAR_SEQ 1..95
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14993207, ECO:0000303|Ref.5"
FT /id="VSP_035969"
SQ SEQUENCE 490 AA; 56827 MW; A12845089AA68003 CRC64;
MKHLLLLRLV LPKPNYILRR SFLFHSGKTT PSPLDPYDTL QRRVARSGDP SASIIKVLDG
WLDQGNLVKT SELHSIIKML RKFSRFSHAL QISDWMSEHR VHEISEGDVA IRLDLIAKVG
GLGEAEKFFE TIPMERRNYH LYGALLNCYA SKKVLHKAEQ VFQEMKELGF LKGCLPYNVM
LNLYVRTGKY TMVEKLLREM EDETVKPDIF TVNTRLHAYS VVSDVEGMEK FLMRCEADQG
LHLDWRTYAD TANGYIKAGL TEKALEMLRK SEQMVNAQKR KHAYEVLMSF YGAAGKKEEV
YRLWSLYKEL DGFYNTGYIS VISALLKMDD IEEVEKIMEE WEAGHSLFDI RIPHLLITGY
CKKGMMEKAE EVVNILVQKW RVEDTSTWER LALGYKMAGK MEKAVEKWKR AIEVSKPGWR
PHQVVLMSCV DYLEGQRDME GLRKILRLLS ERGHISYDQL LYDMNGAGLS WKIVDAMGKG
RYVEEREVRI
