PP16A_HUMAN
ID PP16A_HUMAN Reviewed; 528 AA.
AC Q96I34; D3DWM5;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Protein phosphatase 1 regulatory subunit 16A;
DE AltName: Full=Myosin phosphatase-targeting subunit 3;
DE Flags: Precursor;
GN Name=PPP1R16A; Synonyms=MYPT3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen;
RA Jikuya H., Takano J., Kikuno R., Nagase T., Ohara O.;
RT "The nucleotide sequence of a long cDNA clone isolated from human spleen.";
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung, Placenta, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-433, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Inhibits protein phosphatase 1 activity toward phosphorylase,
CC myosin light chain and myosin substrates. {ECO:0000250}.
CC -!- SUBUNIT: Binds PP1.
CC -!- INTERACTION:
CC Q96I34; Q9BQD7: ANTKMT; NbExp=3; IntAct=EBI-710402, EBI-713602;
CC Q96I34; Q92870-2: APBB2; NbExp=3; IntAct=EBI-710402, EBI-21535880;
CC Q96I34; Q9Y6H3: ATP23; NbExp=3; IntAct=EBI-710402, EBI-12811889;
CC Q96I34; Q5T686: AVPI1; NbExp=3; IntAct=EBI-710402, EBI-8640233;
CC Q96I34; Q8N1L9: BATF2; NbExp=3; IntAct=EBI-710402, EBI-742695;
CC Q96I34; Q8TAB5: C1orf216; NbExp=3; IntAct=EBI-710402, EBI-747505;
CC Q96I34; Q3SXR2: C3orf36; NbExp=3; IntAct=EBI-710402, EBI-18036948;
CC Q96I34; Q8IYX3: CCDC116; NbExp=3; IntAct=EBI-710402, EBI-744311;
CC Q96I34; Q96GN5: CDCA7L; NbExp=3; IntAct=EBI-710402, EBI-5278764;
CC Q96I34; P28329-3: CHAT; NbExp=3; IntAct=EBI-710402, EBI-25837549;
CC Q96I34; P12532: CKMT1B; NbExp=3; IntAct=EBI-710402, EBI-1050662;
CC Q96I34; P22607: FGFR3; NbExp=3; IntAct=EBI-710402, EBI-348399;
CC Q96I34; P14136: GFAP; NbExp=3; IntAct=EBI-710402, EBI-744302;
CC Q96I34; P06396: GSN; NbExp=3; IntAct=EBI-710402, EBI-351506;
CC Q96I34; Q8WUI4-6: HDAC7; NbExp=3; IntAct=EBI-710402, EBI-12094670;
CC Q96I34; P01112: HRAS; NbExp=3; IntAct=EBI-710402, EBI-350145;
CC Q96I34; Q14005-2: IL16; NbExp=3; IntAct=EBI-710402, EBI-17178971;
CC Q96I34; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-710402, EBI-1055254;
CC Q96I34; Q92993: KAT5; NbExp=3; IntAct=EBI-710402, EBI-399080;
CC Q96I34; Q3LI72: KRTAP19-5; NbExp=3; IntAct=EBI-710402, EBI-1048945;
CC Q96I34; P59942: MCCD1; NbExp=3; IntAct=EBI-710402, EBI-11987923;
CC Q96I34; P52815: MRPL12; NbExp=3; IntAct=EBI-710402, EBI-358272;
CC Q96I34; P52179-2: MYOM1; NbExp=3; IntAct=EBI-710402, EBI-12010196;
CC Q96I34; O14561: NDUFAB1; NbExp=3; IntAct=EBI-710402, EBI-1246261;
CC Q96I34; P19404: NDUFV2; NbExp=3; IntAct=EBI-710402, EBI-713665;
CC Q96I34; Q9HC98-4: NEK6; NbExp=3; IntAct=EBI-710402, EBI-11750983;
CC Q96I34; P29474: NOS3; NbExp=3; IntAct=EBI-710402, EBI-1391623;
CC Q96I34; P61970: NUTF2; NbExp=3; IntAct=EBI-710402, EBI-591778;
CC Q96I34; D3DTS7: PMP22; NbExp=3; IntAct=EBI-710402, EBI-25882629;
CC Q96I34; P62140: PPP1CB; NbExp=10; IntAct=EBI-710402, EBI-352350;
CC Q96I34; P36873: PPP1CC; NbExp=3; IntAct=EBI-710402, EBI-356283;
CC Q96I34; O43741: PRKAB2; NbExp=3; IntAct=EBI-710402, EBI-1053424;
CC Q96I34; Q8WWY3: PRPF31; NbExp=3; IntAct=EBI-710402, EBI-1567797;
CC Q96I34; Q9UHX1: PUF60; NbExp=2; IntAct=EBI-710402, EBI-1053259;
CC Q96I34; Q9BWG6: SCNM1; NbExp=3; IntAct=EBI-710402, EBI-748391;
CC Q96I34; O43463: SUV39H1; NbExp=3; IntAct=EBI-710402, EBI-349968;
CC Q96I34; Q08117: TLE5; NbExp=3; IntAct=EBI-710402, EBI-717810;
CC Q96I34; P29144: TPP2; NbExp=4; IntAct=EBI-710402, EBI-1044672;
CC Q96I34; Q15654: TRIP6; NbExp=3; IntAct=EBI-710402, EBI-742327;
CC Q96I34; Q96PN8: TSSK3; NbExp=3; IntAct=EBI-710402, EBI-3918381;
CC Q96I34; Q5W5X9-3: TTC23; NbExp=3; IntAct=EBI-710402, EBI-9090990;
CC Q96I34; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-710402, EBI-741480;
CC Q96I34; Q9H9P5-5: UNKL; NbExp=3; IntAct=EBI-710402, EBI-12817837;
CC Q96I34; Q68DK2-5: ZFYVE26; NbExp=3; IntAct=EBI-710402, EBI-8656416;
CC Q96I34; Q96IQ9: ZNF414; NbExp=3; IntAct=EBI-710402, EBI-744257;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC03452.1; Type=Miscellaneous discrepancy; Note=Cloning artifact.; Evidence={ECO:0000305};
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DR EMBL; AK090471; BAC03452.1; ALT_SEQ; mRNA.
DR EMBL; BT006845; AAP35491.1; -; mRNA.
DR EMBL; CH471162; EAW82080.1; -; Genomic_DNA.
DR EMBL; CH471162; EAW82081.1; -; Genomic_DNA.
DR EMBL; CH471162; EAW82082.1; -; Genomic_DNA.
DR EMBL; BC007854; AAH07854.1; -; mRNA.
DR EMBL; BC049841; AAH49841.1; -; mRNA.
DR EMBL; BC053506; AAH53506.1; -; mRNA.
DR CCDS; CCDS6429.1; -.
DR RefSeq; NP_001316371.1; NM_001329442.1.
DR RefSeq; NP_001316372.1; NM_001329443.1.
DR RefSeq; NP_001316373.1; NM_001329444.1.
DR RefSeq; NP_001316374.1; NM_001329445.1.
DR RefSeq; NP_116291.1; NM_032902.6.
DR RefSeq; XP_016869403.1; XM_017013914.1.
DR AlphaFoldDB; Q96I34; -.
DR SMR; Q96I34; -.
DR BioGRID; 124414; 55.
DR ELM; Q96I34; -.
DR IntAct; Q96I34; 62.
DR MINT; Q96I34; -.
DR STRING; 9606.ENSP00000292539; -.
DR GlyGen; Q96I34; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q96I34; -.
DR PhosphoSitePlus; Q96I34; -.
DR BioMuta; PPP1R16A; -.
DR DMDM; 22256976; -.
DR EPD; Q96I34; -.
DR jPOST; Q96I34; -.
DR MassIVE; Q96I34; -.
DR MaxQB; Q96I34; -.
DR PaxDb; Q96I34; -.
DR PeptideAtlas; Q96I34; -.
DR PRIDE; Q96I34; -.
DR ProteomicsDB; 76809; -.
DR Antibodypedia; 14950; 93 antibodies from 22 providers.
DR DNASU; 84988; -.
DR Ensembl; ENST00000292539.8; ENSP00000292539.4; ENSG00000160972.10.
DR Ensembl; ENST00000435887.2; ENSP00000391126.1; ENSG00000160972.10.
DR GeneID; 84988; -.
DR KEGG; hsa:84988; -.
DR MANE-Select; ENST00000435887.2; ENSP00000391126.1; NM_001329443.2; NP_001316372.1.
DR UCSC; uc003zdd.4; human.
DR CTD; 84988; -.
DR DisGeNET; 84988; -.
DR GeneCards; PPP1R16A; -.
DR HGNC; HGNC:14941; PPP1R16A.
DR HPA; ENSG00000160972; Low tissue specificity.
DR MIM; 609172; gene.
DR neXtProt; NX_Q96I34; -.
DR OpenTargets; ENSG00000160972; -.
DR PharmGKB; PA33634; -.
DR VEuPathDB; HostDB:ENSG00000160972; -.
DR eggNOG; KOG0505; Eukaryota.
DR GeneTree; ENSGT00940000154090; -.
DR HOGENOM; CLU_000134_54_2_1; -.
DR InParanoid; Q96I34; -.
DR OMA; DLEEVCQ; -.
DR PhylomeDB; Q96I34; -.
DR TreeFam; TF316803; -.
DR PathwayCommons; Q96I34; -.
DR SignaLink; Q96I34; -.
DR BioGRID-ORCS; 84988; 12 hits in 1077 CRISPR screens.
DR ChiTaRS; PPP1R16A; human.
DR GenomeRNAi; 84988; -.
DR Pharos; Q96I34; Tdark.
DR PRO; PR:Q96I34; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q96I34; protein.
DR Bgee; ENSG00000160972; Expressed in apex of heart and 162 other tissues.
DR ExpressionAtlas; Q96I34; baseline and differential.
DR Genevisible; Q96I34; HS.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0017020; F:myosin phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0008157; F:protein phosphatase 1 binding; IBA:GO_Central.
DR GO; GO:0019888; F:protein phosphatase regulator activity; IBA:GO_Central.
DR GO; GO:0035304; P:regulation of protein dephosphorylation; IBA:GO_Central.
DR Gene3D; 1.25.40.20; -; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR017417; Pase-1_reg_su_16AB.
DR Pfam; PF12796; Ank_2; 2.
DR PIRSF; PIRSF038159; PP1_16AB_vert; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 4.
DR SUPFAM; SSF48403; SSF48403; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 4.
PE 1: Evidence at protein level;
KW ANK repeat; Cell membrane; Coiled coil; Lipoprotein; Membrane; Methylation;
KW Palmitate; Phosphoprotein; Prenylation; Reference proteome; Repeat.
FT CHAIN 1..525
FT /note="Protein phosphatase 1 regulatory subunit 16A"
FT /id="PRO_0000067040"
FT PROPEP 526..528
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000396707"
FT REPEAT 70..99
FT /note="ANK 1"
FT REPEAT 103..132
FT /note="ANK 2"
FT REPEAT 136..165
FT /note="ANK 3"
FT REPEAT 231..260
FT /note="ANK 4"
FT REPEAT 264..293
FT /note="ANK 5"
FT REGION 19..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..351
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 367..421
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 462..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 18..45
FT /evidence="ECO:0000255"
FT COMPBIAS 41..59
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 382..409
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 433
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 525
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250|UniProtKB:Q923M0"
FT LIPID 524
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 525
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250|UniProtKB:Q923M0"
FT CONFLICT 34..71
FT /note="Missing (in Ref. 1; BAC03452)"
FT /evidence="ECO:0000305"
FT CONFLICT 249
FT /note="A -> P (in Ref. 1; BAC03452)"
FT /evidence="ECO:0000305"
FT CONFLICT 272
FT /note="A -> P (in Ref. 1; BAC03452)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 528 AA; 57811 MW; A5A2D5E1A16A438C CRC64;
MAEHLELLAE MPMVGRMSTQ ERLKHAQKRR AQQVKMWAQA EKEAQGKKGP GERPRKEAAS
QGLLKQVLFP PSVVLLEAAA RNDLEEVRQF LGSGVSPDLA NEDGLTALHQ CCIDDFREMV
QQLLEAGANI NACDSECWTP LHAAATCGHL HLVELLIASG ANLLAVNTDG NMPYDLCDDE
QTLDCLETAM ADRGITQDSI EAARAVPELR MLDDIRSRLQ AGADLHAPLD HGATLLHVAA
ANGFSEAAAL LLEHRASLSA KDQDGWEPLH AAAYWGQVPL VELLVAHGAD LNAKSLMDET
PLDVCGDEEV RAKLLELKHK HDALLRAQSR QRSLLRRRTS SAGSRGKVVR RVSLTQRTDL
YRKQHAQEAI VWQQPPPTSP EPPEDNDDRQ TGAELRPPPP EEDNPEVVRP HNGRVGGSPV
RHLYSKRLDR SVSYQLSPLD STTPHTLVHD KAHHTLADLK RQRAAAKLQR PPPEGPESPE
TAEPGLPGDT VTPQPDCGFR AGGDPPLLKL TAPAVEAPVE RRPCCLLM
