PP175_ARATH
ID PP175_ARATH Reviewed; 738 AA.
AC O82380;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Pentatricopeptide repeat-containing protein At2g29760, chloroplastic;
DE AltName: Full=Protein ORGANELLE TRANSCRIPT PROCESSING 81 {ECO:0000303|PubMed:19934379};
DE Flags: Precursor;
GN Name=PCMP-H33; Synonyms=OTP81 {ECO:0000303|PubMed:19934379};
GN OrderedLocusNames=At2g29760; ORFNames=T27A16.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 69-355.
RC STRAIN=cv. Columbia;
RX PubMed=16244158; DOI=10.1104/pp.105.063479;
RA Xiao Y.-L., Smith S.R., Ishmael N., Redman J.C., Kumar N., Monaghan E.L.,
RA Ayele M., Haas B.J., Wu H.C., Town C.D.;
RT "Analysis of the cDNAs of hypothetical genes on Arabidopsis chromosome 2
RT reveals numerous transcript variants.";
RL Plant Physiol. 139:1323-1337(2005).
RN [4]
RP GENE FAMILY.
RX PubMed=10809006; DOI=10.1023/a:1006352315928;
RA Aubourg S., Boudet N., Kreis M., Lecharny A.;
RT "In Arabidopsis thaliana, 1% of the genome codes for a novel protein family
RT unique to plants.";
RL Plant Mol. Biol. 42:603-613(2000).
RN [5]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=19934379; DOI=10.1105/tpc.109.071472;
RA Hammani K., Okuda K., Tanz S.K., Chateigner-Boutin A.L., Shikanai T.,
RA Small I.;
RT "A study of new Arabidopsis chloroplast RNA editing mutants reveals general
RT features of editing factors and their target sites.";
RL Plant Cell 21:3686-3699(2009).
CC -!- FUNCTION: Involved in RNA editing event in chloroplasts. Required for
CC the editing of a single site in rps12 transcript.
CC {ECO:0000269|PubMed:19934379}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:19934379}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:19934379}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC005496; AAC35225.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08301.1; -; Genomic_DNA.
DR EMBL; AY500319; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; C84700; C84700.
DR RefSeq; NP_180537.1; NM_128530.3.
DR AlphaFoldDB; O82380; -.
DR SMR; O82380; -.
DR STRING; 3702.AT2G29760.1; -.
DR PaxDb; O82380; -.
DR PRIDE; O82380; -.
DR ProteomicsDB; 250502; -.
DR EnsemblPlants; AT2G29760.1; AT2G29760.1; AT2G29760.
DR GeneID; 817526; -.
DR Gramene; AT2G29760.1; AT2G29760.1; AT2G29760.
DR KEGG; ath:AT2G29760; -.
DR Araport; AT2G29760; -.
DR TAIR; locus:2060640; AT2G29760.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_37_2_1; -.
DR InParanoid; O82380; -.
DR OMA; GWIHVYI; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; O82380; -.
DR PRO; PR:O82380; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82380; baseline and differential.
DR Genevisible; O82380; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0031425; P:chloroplast RNA processing; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 1.
DR Pfam; PF13041; PPR_2; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR TIGRFAMs; TIGR00756; PPR; 6.
DR PROSITE; PS51375; PPR; 13.
PE 2: Evidence at transcript level;
KW Chloroplast; Plastid; Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..42
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 43..738
FT /note="Pentatricopeptide repeat-containing protein
FT At2g29760, chloroplastic"
FT /evidence="ECO:0000255"
FT /id="PRO_0000356034"
FT REPEAT 94..129
FT /note="PPR 1"
FT REPEAT 130..164
FT /note="PPR 2"
FT REPEAT 165..195
FT /note="PPR 3"
FT REPEAT 196..230
FT /note="PPR 4"
FT REPEAT 231..265
FT /note="PPR 5"
FT REPEAT 266..296
FT /note="PPR 6"
FT REPEAT 297..331
FT /note="PPR 7"
FT REPEAT 332..363
FT /note="PPR 8"
FT REPEAT 364..398
FT /note="PPR 9"
FT REPEAT 399..429
FT /note="PPR 10"
FT REPEAT 430..464
FT /note="PPR 11"
FT REPEAT 465..495
FT /note="PPR 12"
FT REPEAT 501..531
FT /note="PPR 13"
FT REGION 11..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 536..611
FT /note="Type E motif"
FT REGION 612..642
FT /note="Type E(+) motif"
FT REGION 643..738
FT /note="Type DYW motif"
SQ SEQUENCE 738 AA; 82786 MW; 0CD5BB2CD1ED37E5 CRC64;
MAIFSTAQPL SLPRHPNFSN PNQPTTNNER SRHISLIERC VSLRQLKQTH GHMIRTGTFS
DPYSASKLFA MAALSSFASL EYARKVFDEI PKPNSFAWNT LIRAYASGPD PVLSIWAFLD
MVSESQCYPN KYTFPFLIKA AAEVSSLSLG QSLHGMAVKS AVGSDVFVAN SLIHCYFSCG
DLDSACKVFT TIKEKDVVSW NSMINGFVQK GSPDKALELF KKMESEDVKA SHVTMVGVLS
ACAKIRNLEF GRQVCSYIEE NRVNVNLTLA NAMLDMYTKC GSIEDAKRLF DAMEEKDNVT
WTTMLDGYAI SEDYEAAREV LNSMPQKDIV AWNALISAYE QNGKPNEALI VFHELQLQKN
MKLNQITLVS TLSACAQVGA LELGRWIHSY IKKHGIRMNF HVTSALIHMY SKCGDLEKSR
EVFNSVEKRD VFVWSAMIGG LAMHGCGNEA VDMFYKMQEA NVKPNGVTFT NVFCACSHTG
LVDEAESLFH QMESNYGIVP EEKHYACIVD VLGRSGYLEK AVKFIEAMPI PPSTSVWGAL
LGACKIHANL NLAEMACTRL LELEPRNDGA HVLLSNIYAK LGKWENVSEL RKHMRVTGLK
KEPGCSSIEI DGMIHEFLSG DNAHPMSEKV YGKLHEVMEK LKSNGYEPEI SQVLQIIEEE
EMKEQSLNLH SEKLAICYGL ISTEAPKVIR VIKNLRVCGD CHSVAKLISQ LYDREIIVRD
RYRFHHFRNG QCSCNDFW
