PP178_ARATH
ID PP178_ARATH Reviewed; 918 AA.
AC Q9SIC9;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Pentatricopeptide repeat-containing protein GUN1, chloroplastic {ECO:0000305};
DE AltName: Full=Pentatricopeptide repeat-containing protein At2g31400 {ECO:0000305};
DE AltName: Full=Protein GENOMES UNCOUPLED 1 {ECO:0000303|PubMed:17395793};
DE Flags: Precursor;
GN Name=GUN1 {ECO:0000303|PubMed:17395793};
GN OrderedLocusNames=At2g31400 {ECO:0000312|Araport:AT2G31400};
GN ORFNames=T28P16.11 {ECO:0000312|EMBL:AAD26479.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 544-918.
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=17395793; DOI=10.1126/science. 1140516;
RA Koussevitzky S., Nott A., Mockler T.C., Hong F., Sachetto-Martins G.,
RA Surpin M., Lim J., Mittler R., Chory J.;
RT "Signals from chloroplasts converge to regulate nuclear gene expression.";
RL Science 316:715-719(2007).
RN [6]
RP FUNCTION.
RX PubMed=19140931; DOI=10.1111/j.1469-8137.2008.02729.x;
RA Ruckle M.E., Larkin R.M.;
RT "Plastid signals that affect photomorphogenesis in Arabidopsis thaliana are
RT dependent on GENOMES UNCOUPLED 1 and cryptochrome 1.";
RL New Phytol. 182:367-379(2009).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20605896; DOI=10.1093/jxb/erq186;
RA Cottage A., Mott E.K., Kempster J.A., Gray J.C.;
RT "The Arabidopsis plastid-signalling mutant gun1 (genomes uncoupled1) shows
RT altered sensitivity to sucrose and abscisic acid and alterations in early
RT seedling development.";
RL J. Exp. Bot. 61:3773-3786(2010).
RN [8]
RP FUNCTION.
RX PubMed=21673514; DOI=10.4161/psb.6.4.14900;
RA Cottage A., Gray J.C.;
RT "Timing the switch to phototrophic growth: a possible role of GUN1.";
RL Plant Signal. Behav. 6:578-582(2011).
CC -!- FUNCTION: Involved in nuclear gene expression via the retrograde
CC plastid-to-nucleus signaling (PubMed:17395793, PubMed:19140931,
CC PubMed:20605896, PubMed:21673514). Plastid signals that depend on GUN1
CC can affect photomorphogenesis developments, such as cotyledon opening
CC and expansion, anthocyanin biosynthesis, and hypocotyl elongation
CC (PubMed:19140931, PubMed:21673514). {ECO:0000269|PubMed:17395793,
CC ECO:0000269|PubMed:19140931, ECO:0000269|PubMed:20605896,
CC ECO:0000269|PubMed:21673514}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17395793}.
CC -!- DISRUPTION PHENOTYPE: Defective in retrograde plastid-to-nucleus
CC signaling. {ECO:0000269|PubMed:20605896}.
CC -!- SIMILARITY: Belongs to the PPR family. P subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC007169; AAD26479.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08542.1; -; Genomic_DNA.
DR EMBL; BX819946; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; C84720; C84720.
DR RefSeq; NP_180698.1; NM_128697.3.
DR AlphaFoldDB; Q9SIC9; -.
DR SMR; Q9SIC9; -.
DR STRING; 3702.AT2G31400.1; -.
DR PaxDb; Q9SIC9; -.
DR PRIDE; Q9SIC9; -.
DR ProteomicsDB; 249147; -.
DR EnsemblPlants; AT2G31400.1; AT2G31400.1; AT2G31400.
DR GeneID; 817698; -.
DR Gramene; AT2G31400.1; AT2G31400.1; AT2G31400.
DR KEGG; ath:AT2G31400; -.
DR Araport; AT2G31400; -.
DR TAIR; locus:2061310; AT2G31400.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_49_5_1; -.
DR InParanoid; Q9SIC9; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9SIC9; -.
DR PRO; PR:Q9SIC9; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SIC9; baseline and differential.
DR Genevisible; Q9SIC9; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0010019; P:chloroplast-nucleus signaling pathway; IMP:UniProtKB.
DR GO; GO:0031930; P:mitochondria-nucleus signaling pathway; IMP:TAIR.
DR Gene3D; 1.25.40.10; -; 5.
DR Gene3D; 3.30.1370.110; -; 1.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR002625; Smr_dom.
DR InterPro; IPR036063; Smr_dom_sf.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR Pfam; PF01535; PPR; 2.
DR Pfam; PF13041; PPR_2; 4.
DR Pfam; PF13812; PPR_3; 1.
DR SMART; SM00463; SMR; 1.
DR SUPFAM; SSF160443; SSF160443; 1.
DR TIGRFAMs; TIGR00756; PPR; 9.
DR PROSITE; PS51375; PPR; 12.
DR PROSITE; PS50828; SMR; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Plastid; Reference proteome; Repeat; Transit peptide.
FT TRANSIT 1..41
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 42..918
FT /note="Pentatricopeptide repeat-containing protein GUN1,
FT chloroplastic"
FT /id="PRO_0000356037"
FT REPEAT 232..266
FT /note="PPR 1"
FT REPEAT 267..301
FT /note="PPR 2"
FT REPEAT 302..337
FT /note="PPR 3"
FT REPEAT 338..372
FT /note="PPR 4"
FT REPEAT 373..407
FT /note="PPR 5"
FT REPEAT 408..442
FT /note="PPR 6"
FT REPEAT 443..477
FT /note="PPR 7"
FT REPEAT 478..512
FT /note="PPR 8"
FT REPEAT 513..547
FT /note="PPR 9"
FT REPEAT 548..582
FT /note="PPR 10"
FT REPEAT 583..619
FT /note="PPR 11"
FT DOMAIN 781..873
FT /note="Smr"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00321"
FT REGION 1..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..138
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 918 AA; 101624 MW; 15627FA285D37D8B CRC64;
MASTPPHWVT TTNNHRPWLP QRPRPGRSVT SAPPSSSASV SSAHLSQTTP NFSPLQTPKS
DFSGRQSTRF VSPATNNHRQ TRQNPNYNHR PYGASSSPRG SAPPPSSVAT VAPAQLSQPP
NFSPLQTPKS DLSSDFSGRR STRFVSKMHF GRQKTTMATR HSSAAEDALQ NAIDFSGDDE
MFHSLMLSFE SKLCGSDDCT YIIRELGNRN ECDKAVGFYE FAVKRERRKN EQGKLASAMI
STLGRYGKVT IAKRIFETAF AGGYGNTVYA FSALISAYGR SGLHEEAISV FNSMKEYGLR
PNLVTYNAVI DACGKGGMEF KQVAKFFDEM QRNGVQPDRI TFNSLLAVCS RGGLWEAARN
LFDEMTNRRI EQDVFSYNTL LDAICKGGQM DLAFEILAQM PVKRIMPNVV SYSTVIDGFA
KAGRFDEALN LFGEMRYLGI ALDRVSYNTL LSIYTKVGRS EEALDILREM ASVGIKKDVV
TYNALLGGYG KQGKYDEVKK VFTEMKREHV LPNLLTYSTL IDGYSKGGLY KEAMEIFREF
KSAGLRADVV LYSALIDALC KNGLVGSAVS LIDEMTKEGI SPNVVTYNSI IDAFGRSATM
DRSADYSNGG SLPFSSSALS ALTETEGNRV IQLFGQLTTE SNNRTTKDCE EGMQELSCIL
EVFRKMHQLE IKPNVVTFSA ILNACSRCNS FEDASMLLEE LRLFDNKVYG VVHGLLMGQR
ENVWLQAQSL FDKVNEMDGS TASAFYNALT DMLWHFGQKR GAELVALEGR SRQVWENVWS
DSCLDLHLMS SGAARAMVHA WLLNIRSIVY EGHELPKVLS ILTGWGKHSK VVGDGALRRA
VEVLLRGMDA PFHLSKCNMG RFTSSGSVVA TWLRESATLK LLILHDHITT ATATTTTMKS
TDQQQRKQTS FALQPLLL
