ID   PP1AA_PSEPY             Reviewed;          27 AA.
AC   C0HLS2;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   02-DEC-2020, sequence version 1.
DT   25-MAY-2022, entry version 6.
DE   RecName: Full=DELTA-pseudomyrmecitoxin-Pp1a subunit A {ECO:0000303|PubMed:33344898};
DE            Short=DELTA-PSDTX-Pp1a subunit A {ECO:0000303|PubMed:33344898};
OS   Pseudomyrmex penetrator (Ant).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC   Formicidae; Pseudomyrmecinae; Pseudomyrmex.
OX   NCBI_TaxID=621823 {ECO:0000303|PubMed:33344898};
RN   [1]
RP   PROTEIN SEQUENCE, IDENTIFICATION BY MASS SPECTROMETRY, SYNTHESIS, FUNCTION,
RP   SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND TOXIC DOSE.
RC   TISSUE=Venom {ECO:0000303|PubMed:33344898};
RX   PubMed=33344898; DOI=10.1021/acsptsci.0c00119;
RA   Touchard A., Mendel H.C., Boulogne I., Herzig V., Braga Emidio N.,
RA   King G.F., Triquigneaux M., Jaquillard L., Beroud R., De Waard M.,
RA   Delalande O., Dejean A., Muttenthaler M., Duplais C.;
RT   "Heterodimeric Insecticidal Peptide Provides New Insights into the
RT   Molecular and Functional Diversity of Ant Venoms.";
RL   ACS Pharmacol. Transl. Sci. 3:1211-1224(2020).
CC   -!- FUNCTION: This heterodimer has insecticidal and cytotoxic properties
CC       (PubMed:33344898). Induces immediate paralysis when injected into
CC       blowflies (Lucilia cuprina), and then death within 24 hours
CC       (PubMed:33344898). Also inhibits the growth of Aedes albopictus
CC       mosquito C6/36 cells (PubMed:33344898). {ECO:0000269|PubMed:33344898}.
CC   -!- SUBUNIT: Heterodimer composed of subunit A and subunit B (DELTA-PSDTX-
CC       Pp1a); disulfide-linked. {ECO:0000269|PubMed:33344898}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:33344898}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:33344898}.
CC   -!- TOXIC DOSE: Heterodimer (A and B chains): PD(50) is 2.5 nmol/g in sheep
CC       blowflies (Lucilia cuprina) (at 0.5 hours post-injection).
CC       {ECO:0000269|PubMed:33344898}.
CC   -!- TOXIC DOSE: Heterodimer (A and B chains): LD(50) is 3.0 nmol/g by
CC       injection into the ventro-lateral thorax of sheep blowflies (Lucilia
CC       cuprina). {ECO:0000269|PubMed:33344898}.
CC   -!- TOXIC DOSE: Heterodimer (A and B chains): LD(50) is 1.04 um/ml in Aedes
CC       albopictus mosquito C6/36 cells. {ECO:0000269|PubMed:33344898}.
CC   -!- MISCELLANEOUS: Electrospray ionization experiments give a mass of
CC       6598.6 Da for DELTA-pseudomyrmecitoxin-Pp1a heterodimer (subunits A+B).
CC       {ECO:0000269|PubMed:33344898}.
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DR   AlphaFoldDB; C0HLS2; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IDA:UniProtKB.
DR   GO; GO:0051715; P:cytolysis in another organism; IDA:UniProtKB.
DR   GO; GO:0002213; P:defense response to insect; IDA:UniProtKB.
DR   GO; GO:0035738; P:envenomation resulting in modulation of process in another organism; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Toxin.
FT   CHAIN           1..27
FT                   /note="DELTA-pseudomyrmecitoxin-Pp1a subunit A"
FT                   /id="PRO_0000451569"
FT   DISULFID        14
FT                   /note="Interchain (with C-28 in subunit B)"
FT                   /evidence="ECO:0000269|PubMed:33344898"
FT   DISULFID        24
FT                   /note="Interchain (with C-18 in subunit B)"
FT                   /evidence="ECO:0000269|PubMed:33344898"
SQ   SEQUENCE   27 AA;  2983 MW;  F61BBF9D8063EC2C CRC64;
     KIPNILKGGL KSICKHRKYL DKACAAI