PP1_ORYSJ
ID PP1_ORYSJ Reviewed; 322 AA.
AC P48489; Q10NJ4; Q84J82;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2006, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Serine/threonine-protein phosphatase PP1;
DE EC=3.1.3.16;
GN OrderedLocusNames=Os03g0268000, LOC_Os03g16110;
GN ORFNames=OJ1364E02.17, OJA1364E02.7;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Seed;
RX PubMed=10080713; DOI=10.1023/a:1006152223183;
RA Chang M., Wang B., Chen X., Wu R.;
RT "Molecular characterization of catalytic-subunit cDNA sequences encoding
RT protein phosphatases 1 and 2A and study of their roles in the gibberellin-
RT dependent Osamy-c expression in rice.";
RL Plant Mol. Biol. 39:105-115(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] +
CC phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:83421; EC=3.1.3.16;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] +
CC phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA-
CC COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:61977; EC=3.1.3.16;
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 2 manganese ions per subunit. {ECO:0000250};
CC -!- SIMILARITY: Belongs to the PPP phosphatase family. PP-1 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAP06889.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAP06897.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U31773; AAA74625.1; -; mRNA.
DR EMBL; AC135208; AAP06889.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC139168; AAP06897.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000009; ABF95164.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF95165.1; -; Genomic_DNA.
DR EMBL; DP000009; ABF95166.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF11583.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS83435.1; -; Genomic_DNA.
DR EMBL; AK073140; BAG93306.1; -; mRNA.
DR PIR; T03304; T03304.
DR AlphaFoldDB; P48489; -.
DR SMR; P48489; -.
DR STRING; 4530.OS03T0268000-01; -.
DR PaxDb; P48489; -.
DR PRIDE; P48489; -.
DR EnsemblPlants; Os03t0268000-01; Os03t0268000-01; Os03g0268000.
DR Gramene; Os03t0268000-01; Os03t0268000-01; Os03g0268000.
DR eggNOG; KOG0374; Eukaryota.
DR HOGENOM; CLU_004962_0_0_1; -.
DR InParanoid; P48489; -.
DR OMA; EEHEIRY; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR ExpressionAtlas; P48489; baseline and differential.
DR Genevisible; P48489; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017018; F:myosin phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0004722; F:protein serine/threonine phosphatase activity; IBA:GO_Central.
DR GO; GO:0016311; P:dephosphorylation; IEA:EnsemblPlants.
DR GO; GO:0009740; P:gibberellic acid mediated signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0006468; P:protein phosphorylation; IEA:EnsemblPlants.
DR GO; GO:0010161; P:red light signaling pathway; IEA:EnsemblPlants.
DR Gene3D; 3.60.21.10; -; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR006186; Ser/Thr-sp_prot-phosphatase.
DR InterPro; IPR031675; STPPase_N.
DR Pfam; PF00149; Metallophos; 1.
DR Pfam; PF16891; STPPase_N; 1.
DR PRINTS; PR00114; STPHPHTASE.
DR SMART; SM00156; PP2Ac; 1.
DR SUPFAM; SSF56300; SSF56300; 1.
DR PROSITE; PS00125; SER_THR_PHOSPHATASE; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Manganese; Metal-binding; Protein phosphatase;
KW Reference proteome.
FT CHAIN 1..322
FT /note="Serine/threonine-protein phosphatase PP1"
FT /id="PRO_0000058809"
FT ACT_SITE 136
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 75
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 77
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 103
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 103
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 135
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 184
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 259
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT CONFLICT 3..11
FT /note="AAPGAGGQG -> PRRR (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 17
FT /note="A -> P (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 25..26
FT /note="RR -> PP (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 40
FT /note="Q -> H (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 51
FT /note="T -> S (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="A -> G (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 157
FT /note="V -> M (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 199..201
FT /note="SLP -> RLA (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
FT CONFLICT 215..216
FT /note="LL -> YF (in Ref. 1; AAA74625)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 322 AA; 36166 MW; F4C8280134836BE7 CRC64;
MAAAPGAGGQ GGGGMDAVLL DDIIRRLLEV RTARPGKQVQ LSESEIRQLC TVSREIFLSQ
PNLLELEAPI KICGDIHGQY SDLLRLFEYG GFPPEANYLF LGDYVDRGKQ SLETICLLLA
YKIKYPENFF LLRGNHECAS INRIYGFYDE CKRRFNVRLW KVFTDCFNCL PVAALIDDKI
LCMHGGLSPD LTHLDEIKSL PRPTDVPDTG LLCDLLWSDP GKDVQGWGMN DRGVSYTFGA
DKVSEFLEKH DLDLICRAHQ VVEDGYEFFA DRQLVTIFSA PNYCGEFDNA GAMMSVDETL
MCSFQILKPA ERKGKFMASN KM
