PP220_ASFWA
ID PP220_ASFWA Reviewed; 2475 AA.
AC P0CA04;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 1.
DT 03-AUG-2022, entry version 27.
DE RecName: Full=Polyprotein pp220 {ECO:0000250|UniProtKB:Q08358};
DE AltName: Full=220 kDa polyprotein;
DE Contains:
DE RecName: Full=p34 {ECO:0000250|UniProtKB:Q08358};
DE Contains:
DE RecName: Full=p14 {ECO:0000250|UniProtKB:Q08358};
DE Contains:
DE RecName: Full=p37 {ECO:0000250|UniProtKB:Q08358};
DE Contains:
DE RecName: Full=p150 {ECO:0000250|UniProtKB:Q08358};
DE Contains:
DE RecName: Full=p5 {ECO:0000250|UniProtKB:Q08358};
DE Flags: Precursor;
GN OrderedLocusNames=War-102;
OS African swine fever virus (isolate Warthog/Namibia/Wart80/1980) (ASFV).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Asfuvirales; Asfarviridae; Asfivirus.
OX NCBI_TaxID=561444;
OH NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH NCBI_TaxID=9823; Sus scrofa (Pig).
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Kutish G.F., Rock D.L.;
RT "African swine fever virus genomes.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: [Polyprotein pp220]: Essential for the core assembly. Its
CC myristoyl moiety may function as a membrane-anchoring signal to bind
CC the developing core shell to the inner viral envelope.
CC {ECO:0000250|UniProtKB:Q08358}.
CC -!- FUNCTION: [p34]: The structural protein p34 is a component of the virus
CC core shell. {ECO:0000250|UniProtKB:Q08358}.
CC -!- FUNCTION: [p14]: The structural protein p14 is a component of the virus
CC core shell. {ECO:0000250|UniProtKB:Q08358}.
CC -!- FUNCTION: [p37]: The structural protein p37 is a component of the virus
CC core shell. {ECO:0000250|UniProtKB:Q08358}.
CC -!- FUNCTION: [p150]: The structural protein p150 is a component of the
CC virus core shell. {ECO:0000250|UniProtKB:Q08358}.
CC -!- SUBCELLULAR LOCATION: [Polyprotein pp220]: Host cytoplasm, host
CC perinuclear region {ECO:0000250|UniProtKB:Q08358}. Note=Found in
CC perinuclear cytoplasmic viral factories during assembly.
CC {ECO:0000250|UniProtKB:Q08358}.
CC -!- SUBCELLULAR LOCATION: [p34]: Virion {ECO:0000250|UniProtKB:Q08358}.
CC Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}.
CC Note=Localizes to the viral factory at 16 hpi (By similarity). In the
CC virion, located in the core shell, which functions like a matrix
CC between the DNA-containing nucleoid and the inner envelope (By
CC similarity). {ECO:0000250|UniProtKB:Q08358}.
CC -!- SUBCELLULAR LOCATION: [p14]: Virion {ECO:0000250|UniProtKB:Q08358}.
CC Host cytoplasm, host perinuclear region. Note=Found in perinuclear
CC cytoplasmic viral factories during assembly. In the virion, located in
CC the core shell, which functions like a matrix between the DNA-
CC containing nucleoid and the inner envelope (By similarity).
CC {ECO:0000250|UniProtKB:Q08358}.
CC -!- SUBCELLULAR LOCATION: [p37]: Virion {ECO:0000250|UniProtKB:Q08358}.
CC Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}.
CC Host nucleus {ECO:0000250|UniProtKB:Q08358}. Note=Found in perinuclear
CC cytoplasmic viral factories during assembly. In the virion, located in
CC the core shell, which functions like a matrix between the DNA-
CC containing nucleoid and the inner envelope (By similarity).
CC {ECO:0000250|UniProtKB:Q08358}.
CC -!- SUBCELLULAR LOCATION: [p150]: Virion {ECO:0000250|UniProtKB:Q08358}.
CC Host cytoplasm, host perinuclear region {ECO:0000250|UniProtKB:Q08358}.
CC Note=Found in perinuclear cytoplasmic viral factories during assembly.
CC In the virion, located in the core shell, which functions like a matrix
CC between the DNA-containing nucleoid and the inner envelope (By
CC similarity). {ECO:0000250|UniProtKB:Q08358}.
CC -!- SUBCELLULAR LOCATION: [p5]: Virion {ECO:0000250|UniProtKB:Q08358}.
CC -!- INDUCTION: [Polyprotein pp220]: Expressed in the late phase of the
CC viral replicative cycle. {ECO:0000250|UniProtKB:Q08358}.
CC -!- PTM: [Polyprotein pp220]: The polyprotein is not glycosylated.
CC -!- PTM: [Polyprotein pp220]: Specific enzymatic cleavages in vivo by the
CC viral pS273R protease yield mature proteins.
CC {ECO:0000250|UniProtKB:Q08358}.
CC -!- SIMILARITY: Belongs to the asfivirus polyprotein pp220 family.
CC {ECO:0000305}.
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DR EMBL; AY261366; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR Proteomes; UP000000858; Genome.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0044220; C:host cell perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
PE 3: Inferred from homology;
KW Coiled coil; Host cytoplasm; Host nucleus; Late protein; Lipoprotein;
KW Myristate; Virion.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..44
FT /note="p5"
FT /id="PRO_0000454849"
FT CHAIN 45..2475
FT /note="Polyprotein pp220"
FT /evidence="ECO:0000255"
FT /id="PRO_0000373440"
FT CHAIN 46..368
FT /note="p34"
FT /evidence="ECO:0000255"
FT /id="PRO_0000373441"
FT CHAIN 369..522
FT /note="p14"
FT /evidence="ECO:0000255"
FT /id="PRO_0000373442"
FT CHAIN 523..893
FT /note="p37"
FT /evidence="ECO:0000255"
FT /id="PRO_0000373443"
FT CHAIN 894..2475
FT /note="p150"
FT /evidence="ECO:0000255"
FT /id="PRO_0000373444"
FT COILED 2184..2211
FT /evidence="ECO:0000255"
FT SITE 44..45
FT /note="Cleavage; by viral protease S273R"
FT /evidence="ECO:0000255"
FT SITE 368..369
FT /note="Cleavage; by viral protease S273R"
FT /evidence="ECO:0000255"
FT SITE 522..523
FT /note="Cleavage; by viral protease S273R"
FT /evidence="ECO:0000255"
FT SITE 893..894
FT /note="Cleavage; by viral protease S273R"
FT /evidence="ECO:0000255"
FT LIPID 2
FT /note="N-myristoyl glycine; by host"
FT /evidence="ECO:0000250"
SQ SEQUENCE 2475 AA; 280995 MW; D29FA9A26C74BB23 CRC64;
MGNRGSSTSS RPPPSSEANI YAKLQDHIQR QTRPFSGGGY FNGGGDKNPV QHIKDYHIDS
VSSKAKLRII EGIIRAIAKI GFKVDTKQPI EDILKDIKKQ LPDPRAGSTF VKNAEKQETV
CKMIADAINQ EFIDLGQDKL IDTTEGAASI CRQIVLYINS LTHGLRAEYL DVHGSIENTL
ENIKLLNDAI KQLHERMVTE VTKAAPNEEV INAVTMIEAV YRRLLNEQNL QINILTNFID
NILTPTQKEL DKLQTDEVDI IKLLNDTNSV LGTKNFGKVL SYTLCNLGIA ASVANKINKA
LQKVGLKVEQ YLQSKNWAEF DKELDLKRFS GLVSAENIAE FEKAVNLLRQ TFNERHKILE
NSCAKKGGDE EKTPLDRRIE AQRLDRKHIL MEFLNKSTQA YNDFLENVKK IGIKLVKEIA
LTPNITRLRD ALSRINDMGT IALDLSLIGF YTNAAAREER ETFLTQFMLV KNVLEEQSKI
DPNFKNLYDS CSRLLQIIDF YTDIVQKKYG GGEDCECTRV GGAALTVEEL GLSKAARSQV
DLNQAINTFM YYYYVAQIYS NLTHNKQEFQ SYEENYATIL GDAIAGRLMQ LDTEKNARIN
SPAVDLARGH VGPNPGGAQE EDWKATVSAI ELEYDVKRRF YRALEGLDLY LKNITKTFVN
NIDSIQTVQQ MLDGVRIIGR WFTEATGDTL AQVFESFPTS AGNDSNVFTD NAPAGHYYEK
VAAEIQQGRS VGTLRPVRAS QAKNIRDLIG RSLSNFQALK NIINAFARIG DMLGGEELRQ
MVPMSPLQIY KTLLEYIQHS ALSVGLKNLN QSEIGGQRVA LARTPEEAAQ RVYLSTVRVN
DALSTRWETE DVFFTFMLKS MAAKIFIVLG IYDMFERPEP VYKLIPTRMI LGGADELEPE
VIPEAAELYF RLPRLAEFYQ KLFSFRDENV QISMLPELEG IFSGLIRIIF MRPIELINIG
DYSETEIRQL IKEINVIYQH FNLEYGEQEA TKKALIHFVN EINRRFGVIT RTEWEKFQRI
VQEARTMNDF GMMNQTNYSI LPDEDGYTQS SQLLPSDRFI SPSSQPTPKW RPALYNIDSV
DVQTGMLQPN SQWDLVQKFR KQLSEMFEDP SLQQELGKVS YQELIRQAIN ELKKDHTDKI
QIVSKLIQGS ESLADTDVNK IFLFHETVIT GLNLLSAIYV LLNNFRNNIK GLDLDTIQKS
IIEWLRETQA ANVNHANLID WLGRKHGAIS EIRNPGLVVK ENDARLSRVY PDPTTNATAP
QDQNLVTETL FAWIVPYVGI PAGGGVRAEQ ELAARYLVDN QRIMQLLLTN IFEMTSSFNK
MVQVRFPETS TAQVHLDFTG LISLIDSLMA DTKYFLNLLR PHIDKNIIQY YENRSNPGSF
YWLEEHLIDK LIKPPTDAGG RPLPGGELGL EGVNQIINKT YTLLTKPYNV LQLQGGAQRR
DAANIQINNN PQPSERFEQY GRVFSRLVFY DALENNSGLR VEQVVLGDFR LSNLIRTNNA
QEENALSYWD NIALRTYANV NDAANNLRRY RLYGSDHGIQ NNRSMMMVFN QLVASYIARF
YDAPSGKIYL NLINAFANGN FSQAVMEMGY AHPDLARNNN AFGHRGDPTE QSVLLLSLGL
ILQRLIKDTN RQGLSQHLIS TLTEIPIYLK ENYRANLPLF NKMFNILISQ GELLKQFIQY
TNVQLARPNL MGLLGANNDS IIYYNNNNVP TTGLSVGQAA LRGIGGVFRP NVTLMPLGDA
QNNTSDIVRK RLVAVIDGII RGSHTLADSA MEVLHELTDH PIYLETEEHF IQNYMSRYNK
EPLMPFSLSL YYLRDLRIEN NEVYDPLLYP NLESGSPEFK LLYGTRKLLG NDPVQLSDMP
GVQLIMKNYN ETVVAREQIT PTRFEHFYTH AIQALRFIIN IRSFKTVMMY NENTFGGVNL
ISENRDDKPI ITAGIGMNAV YSLRKTLQDV ISFVESSYQE EQINHIHKIV SPKGQTRTLG
SNRERERIFN LFDMNIIPIN VNALMRSIPL ANIYNYDYSF EEIACLMYGI SAEKVRSLDT
TAPQPDVAEV LNIPNRPPIN TREFMLKLLI NPYVSVSITQ YGNELLSKGN AGYMSRIFRG
DNALNMGRPK FLSDQIFNKV LFGSLYPTQF DYDEAGPGLA AGIQRGRERW GHPMSIYINQ
ALHEIVRTIR LAETVRGLRN VIDRNQIIGE LNAFRTQLED TRREVNNLIQ TPEIQNNPTP
EIIAAIQNWV QQYRGQITNL IDLIGNAGQA NSMINLIQNI TPQTAGAQLT ALFNIRGLPA
PPPRQPLQND IEAMQWFMTI VINHPPILIA PFMLLVNNLK EFLNTLERYV YKTPRWLGPG
TARIAQPPVG MAPGINMRHH TSYTENSVLT YITEQNREEG PWSIVKQVGV GIQKPTLVHI
GKDRFDTRLI RNLIFITNIQ RLLRLRLNLE LSQFRNVLVS PDHIINPSIT EYGFSITGPS
ETFSDKQYDS DIRIL
