PP223_ARATH
ID PP223_ARATH Reviewed; 623 AA.
AC Q9CAY1;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Putative pentatricopeptide repeat-containing protein At3g11460, mitochondrial;
DE AltName: Full=Mitochondrial RNA editing factor 10 {ECO:0000303|PubMed:23288601};
DE Flags: Precursor;
GN Name=PCMP-H52; Synonyms=MEF10 {ECO:0000303|PubMed:23288601};
GN OrderedLocusNames=At3g11460; ORFNames=F24K9.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND SUBCELLULAR LOCATION.
RX PubMed=15269332; DOI=10.1105/tpc.104.022236;
RA Lurin C., Andres C., Aubourg S., Bellaoui M., Bitton F., Bruyere C.,
RA Caboche M., Debast C., Gualberto J., Hoffmann B., Lecharny A., Le Ret M.,
RA Martin-Magniette M.-L., Mireau H., Peeters N., Renou J.-P., Szurek B.,
RA Taconnat L., Small I.;
RT "Genome-wide analysis of Arabidopsis pentatricopeptide repeat proteins
RT reveals their essential role in organelle biogenesis.";
RL Plant Cell 16:2089-2103(2004).
RN [4]
RP FUNCTION, AND INTERACTION WITH MORF8/RIP1.
RX PubMed=23288601; DOI=10.1007/s11103-012-0003-2;
RA Haertel B., Zehrmann A., Verbitskiy D., van der Merwe J.A., Brennicke A.,
RA Takenaka M.;
RT "MEF10 is required for RNA editing at nad2-842 in mitochondria of
RT Arabidopsis thaliana and interacts with MORF8.";
RL Plant Mol. Biol. 81:337-346(2013).
CC -!- FUNCTION: Involved in C-to-U editing of mitochondrial RNA. Required
CC specifically for editing the mitochondrial NAD2 transcript.
CC {ECO:0000269|PubMed:23288601}.
CC -!- SUBUNIT: Interacts with MORF8/RIP1. {ECO:0000269|PubMed:23288601}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:15269332}.
CC -!- SIMILARITY: Belongs to the PPR family. PCMP-H subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Pentatricopeptide repeat proteins;
CC URL="https://ppr.plantenergy.uwa.edu.au";
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DR EMBL; AC008153; AAG51440.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75049.1; -; Genomic_DNA.
DR RefSeq; NP_187753.1; NM_111979.1.
DR AlphaFoldDB; Q9CAY1; -.
DR SMR; Q9CAY1; -.
DR BioGRID; 5653; 1.
DR STRING; 3702.AT3G11460.1; -.
DR PaxDb; Q9CAY1; -.
DR PRIDE; Q9CAY1; -.
DR ProteomicsDB; 248946; -.
DR EnsemblPlants; AT3G11460.1; AT3G11460.1; AT3G11460.
DR GeneID; 820319; -.
DR Gramene; AT3G11460.1; AT3G11460.1; AT3G11460.
DR KEGG; ath:AT3G11460; -.
DR Araport; AT3G11460; -.
DR TAIR; locus:2080727; AT3G11460.
DR eggNOG; KOG4197; Eukaryota.
DR HOGENOM; CLU_002706_37_8_1; -.
DR InParanoid; Q9CAY1; -.
DR OMA; KDPGYSY; -.
DR OrthoDB; 1344243at2759; -.
DR PhylomeDB; Q9CAY1; -.
DR PRO; PR:Q9CAY1; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9CAY1; baseline and differential.
DR Genevisible; Q9CAY1; AT.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0016554; P:cytidine to uridine editing; IMP:TAIR.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0009451; P:RNA modification; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR032867; DYW_dom.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR045215; PPR_prot_plant.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR24015; PTHR24015; 1.
DR Pfam; PF14432; DYW_deaminase; 1.
DR Pfam; PF01535; PPR; 3.
DR Pfam; PF13041; PPR_2; 3.
DR TIGRFAMs; TIGR00756; PPR; 6.
DR PROSITE; PS51375; PPR; 13.
PE 1: Evidence at protein level;
KW Mitochondrion; mRNA processing; Reference proteome; Repeat;
KW Transit peptide.
FT TRANSIT 1..35
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 36..623
FT /note="Putative pentatricopeptide repeat-containing protein
FT At3g11460, mitochondrial"
FT /id="PRO_0000356082"
FT REPEAT 17..51
FT /note="PPR 1"
FT REPEAT 52..86
FT /note="PPR 2"
FT REPEAT 87..117
FT /note="PPR 3"
FT REPEAT 120..154
FT /note="PPR 4"
FT REPEAT 155..189
FT /note="PPR 5"
FT REPEAT 190..220
FT /note="PPR 6"
FT REPEAT 221..255
FT /note="PPR 7"
FT REPEAT 256..290
FT /note="PPR 8"
FT REPEAT 291..321
FT /note="PPR 9"
FT REPEAT 322..356
FT /note="PPR 10"
FT REPEAT 357..387
FT /note="PPR 11"
FT REPEAT 393..423
FT /note="PPR 12"
FT REGION 428..503
FT /note="Type E motif"
FT REGION 504..535
FT /note="Type E(+) motif"
FT REGION 536..623
FT /note="Type DYW motif"
SQ SEQUENCE 623 AA; 69127 MW; EDF4A2CACE9157F6 CRC64;
MIVVTSFVRN SAVAAVASTP WNVRLRELAY QSLFSESISL YRSMLRSGSS PDAFSFPFIL
KSCASLSLPV SGQQLHCHVT KGGCETEPFV LTALISMYCK CGLVADARKV FEENPQSSQL
SVCYNALISG YTANSKVTDA AYMFRRMKET GVSVDSVTML GLVPLCTVPE YLWLGRSLHG
QCVKGGLDSE VAVLNSFITM YMKCGSVEAG RRLFDEMPVK GLITWNAVIS GYSQNGLAYD
VLELYEQMKS SGVCPDPFTL VSVLSSCAHL GAKKIGHEVG KLVESNGFVP NVFVSNASIS
MYARCGNLAK ARAVFDIMPV KSLVSWTAMI GCYGMHGMGE IGLMLFDDMI KRGIRPDGAV
FVMVLSACSH SGLTDKGLEL FRAMKREYKL EPGPEHYSCL VDLLGRAGRL DEAMEFIESM
PVEPDGAVWG ALLGACKIHK NVDMAELAFA KVIEFEPNNI GYYVLMSNIY SDSKNQEGIW
RIRVMMRERA FRKKPGYSYV EHKGRVHLFL AGDRSHEQTE EVHRMLDELE TSVMELAGNM
DCDRGEEVSS TTREHSERLA IAFGILNSIP GTEILVIKNL RVCEDCHVFL KQVSKIVDRQ
FVVRDASRFH YFKDGVCSCK DYW
